天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 193-196.doi: 10.11958/59112

• 实验研究 • 上一篇    下一篇

孕期脂多糖刺激对子代大鼠胰岛素抵抗的影响

黄丛富， 郝雪琴， 邓雯△   

  1. 洛阳， 河南科技大学动物科技学院药学系 （邮编 471003）
  • 收稿日期:2015-06-17 修回日期:2015-08-28 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △通讯作者 E-mail:wen.dkj@163.com E-mail:727143075@qq.com
  • 基金资助:
    河南科技大学博士科研启动项目 （09001575）； 实验技术开发基金项目 （SY1112046）

Effects of prenatal exposure to lipopolysaccharide on insulin resistance in offspring rats

HUANG Congfu, HAO Xueqin， DENG Wen△   

  1. Department of Pharmacy College of Animal Science and Technology, Henan University of Science and Technology,Luoyang 471003, China
  • Received:2015-06-17 Revised:2015-08-28 Published:2016-02-15 Online:2016-02-15
  • Contact: △Corresponding Author E-mail: wen.dkj@163.com E-mail:727143075@qq.com
  • Supported by:
    Doctor's Scientific research fund

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摘要： 目的 探讨孕期脂多糖（LPS）对子代成年大鼠胰岛素抵抗的影响。方法 SD 孕鼠 20 只随机分为 2 组，每组 10 只。LPS 组孕期第 8、 10、 12 天腹腔注射 LPS 0.40 mg/kg， 对照组腹腔注射无菌生理盐水 0.5 mL。子鼠 3 月龄时测血糖、 血胰岛素的浓度和瘦素（Leptin）水平， 计算稳态胰岛素抵抗指数（HOMA-IR）和胰岛素敏感指数（QUICKI）。结果 与对照组相比， 3 月龄 LPS 组子鼠血糖 （mmol/L：7.72±0.42 vs 7.02±0.42）、 血胰岛素 （mIU/L： 8.78± 4.10 vs 1.51±0.27）、 血 Leptin 水平 （μg/L： 3.88±1.40 vs 1.00±0.33）、 HOMA-IR 值 （3.01±1.41 vs 0.47±0.09） 明显高于对照组； LPS 组 QUICKI 值明显低于对照组 （0.57±0.07 vs 0.99±0.08）。结论 孕期 LPS 刺激会引起子代大鼠胰岛素抵抗。

关键词: 受孕期, 炎症, 脂多糖类, 大鼠, Sprague-Dawley, 子代大鼠, 胰岛素抵抗

Abstract: Objective To explore the effects of prenatal exposure to lipopolysaccharide (LPS) on insulin resistance in adult offspring rats. Methods Nulliparous, time-matched Sprague-Dawley rats were randomly divided into two groups (n= 10 for each group): control group and LPS group. On the pregnant day 8, 10 and 12, rats in control group and LPS group were administered intraperitoneally with saline 0.5 mL or LPS 0.40 mg/kg, respectively. The blood levels of fasting plasma glucose (FPG), insulin and Leptin were detected at 90 days of age in offspring. The steady state insulin resistance index (HOMA-IR) and insulin sensitivity index (QUICKI) were evaluated in adult offspring rats. Results Compared with the control group, blood levels of FPG (mmol/L: 7.72±0.42 vs 7.02±0.42), insulin (mIU/L: 8.78±4.10 vs 1.51±0.27) and Leptin (μg/L: 3.88± 1.40 vs 1.00±0.33) were significantly increased in offsping of LPS group, as well as HOMA-IR (3.01±1.41 vs 0.47±0.09) in⁃ creased, while QUICKI (0.57±0.07 vs 0.99±0.08) decreased in offsping of LPS-treated rats. Conclusion Prenatal exposure to LPS can result in abnormality of insulin resistance in offspring rats.

Key words: fertile period, inflammation, lipopolysaccharides, rats, Sprague-Dawley, offspring rats； insulin resistance