天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (10): 1229-1232.doi: 10.11958/20160029

• 实验研究 • 上一篇    下一篇

医用气源汲送装置复制呼吸衰竭犬模型的可行性研究

王合荣  孙昕 吴琦 付莎莎   

  1. 天津市海河医院， 天津市呼吸疾病研究所， 国家中医药管理局中医药防治传染病重点研究室（邮编 300350）
  • 收稿日期:2016-01-22 修回日期:2016-07-01 出版日期:2016-10-15 发布日期:2016-10-21
  • 通讯作者: 孙昕 E-mail:cassandra1956@163.com
  • 作者简介:王合荣（1982）， 女， 医师， 硕士， 主要从事呼吸系统疾病研究
  • 基金资助:
    天津市卫生局重点攻关项目（11KG126）

Study of the feasibility on medical gas suction feeding device for replicating model of respiratory failure in dogs

WANG Herong, WU Qi, SUN Xin△, FU Shasha   

  1. Tianjin Haihe Hospital, Tianjin Institute of Respiratory Diseases, Traditional Chinese Medicine （TCM） Key Research Laboratory for Infectious Disease Prevention for State Administration of TCM, Tianjin 300350, China
  • Received:2016-01-22 Revised:2016-07-01 Published:2016-10-15 Online:2016-10-21
  • Contact: SUN Xin E-mail:cassandra1956@163.com

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摘要： 目的 探讨医用气源汲送装置复制呼吸衰竭犬模型的可行性及稳定性。 方法 以犬为研究对象， 采用自行设计的气源汲送装置， 通过调控吸入氧气（O2）、二氧化碳（CO2）和氮气（N2）的比例， 降低吸入氧气浓度（FiO2）和（或）提高吸入 CO2浓度（FiCO2）， 同时开放呼出气通路的方法， 使犬动脉血氧分压［p（O2）］和动脉血二氧化碳分压［p（CO2）］达到并稳定在某一设定状态。 分别在基础状态及造模后 1 h 和 2 h 时采集动脉血， 检测相关血气指标。 结果 造模 1 h 后， 实验动物 p（O2）＜ 60 mmHg（1 mmHg=0.133 kPa）、p（CO2）＞ 50 mmHg， 呼吸衰竭犬模型建立成功。 造模 1 h 和 2 h 时酸碱度（pH）值、血氧含量、动脉血氧饱和度（SaO2）、碱剩余（BE）较基础状态降低， p（CO2）、二氧化碳总量（TCO2）、碳酸氢根（HCO3-）较基础状态升高（P < 0.05）。 造模 2 h 时 p（O2）、血氧含量、 SaO2较造模 1 h 降低（P < 0.05）， 而 pH 值、p（CO2）、TCO2、HCO3-、BE 较造模 1 h 差异无统计学意义（P > 0.05）。 结论 在医用气源汲送装置调控下， 成功建立了大型实验动物的呼吸衰竭模型。

关键词: 医用气源汲送装置, 呼吸衰竭, 模型, 动物, 狗, 可行性

Abstract: Objective To investigate the feasibility and stability of medical gas suction feeding device for copying the model of respiratory failure in dogs. Methods The self-designed medical gas suction feeding device was used to establish the model of respiratory failure in dogs. After regulating the proportion of oxygen (O 2), carbon dioxide (CO2) and nitrogen (N2), the fraction of inspiration O2 (FiO2) was decreased, while the forced inspiratory carbon dioxide (FiCO2) was increased. At the same time, the breathing out pathway was open to make the arterial oxygen partial pressure p (O2) and arterial blood carbon dioxide partial pressure p (CO2) reached and stabilized in a stable condition. The arterial blood samples between default state and 1 hour, 2 hour after modeling were collected to detect the blood gas index. Results One hour after the establishment of model, the p (O2) was ≤ 60 mmHg (1 mmHg=0.133 kPa), and p (CO2) was ≥50 mmHg (1 mmHg=0.133 kPa), which suggested that the dog model of respiratory failure was established successfully. Compared with the basic state, data of pH, oxygen content, oxygen saturation (SaO 2) and residual alkali (BE) were decreased at 1 hour and 2 hour after modeling (P < 0.05). The p (O2), oxygen content, SaO2 were decreased in 2 hour after modeling compared with those of 1h after modeling (P < 0.05). There were no significant differences in pH, p (CO2), TCO2, HCO3- and BE between 1 hour and 2 hour after modeling (P > 0.05). Conclusion Under the regulation of the medical gas suction feeding device, the respiratory failure state of large experimental animals is successfully established.

Key words: medical gas suction feeding device, respiratory failure, models, animal, dogs, feasibility