天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (6): 648-651.doi: 10.11958/20180116

• 临床研究 • 上一篇    下一篇

儿童肺炎支原体肺炎肺泡灌洗液中细胞因子分析

郭伟，张同强，徐勇胜，刘福军   

  1. 天津市儿童医院呼吸科（邮编300134）
  • 收稿日期:2018-01-19 修回日期:2018-03-07 出版日期:2018-06-15 发布日期:2018-07-05
  • 通讯作者: 郭伟 E-mail:guowei79656@126.com

The study on cytokines of bronchoalveolar lavage fluid in children with mycoplasma pneumoniae pneumonia

GUO Wei, ZHANG Tong-qiang, XU Yong-sheng, LIU Fu-jun   

  1. Department of Respiratory Medicine, Tianjin Children’s Hospital, Tianjin 300134, China
  • Received:2018-01-19 Revised:2018-03-07 Published:2018-06-15 Online:2018-07-05

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摘要： 目的 研究儿童肺炎支原体肺炎（MPP）肺泡灌洗液（BALF）中细胞因子的表达，以进一步研究MPP的发病机制。方法 以90例MPP患儿作为研究对象（MPP组，A组），依据病程分为A1组（<7 d）、A2组（7～14 d）和A3组（15～30 d），每组30例。对照组（B组，30例）为同期非MPP且需经纤维支气管镜检查或者治疗的患儿。留取BALF进行相关细胞因子的测定。结果 A组患儿BALF中肿瘤坏死因子（TNF）-α、γ-干扰素（IFN-γ）、白细胞介素（IL）-2、IL-4、IL-6和IL-10水平均高于B组，差异有统计学意义（P＜0.05）。TNF-α、IFN-γ、IL-2在A1组升高最明显；IL-4、IL-6在A2组最高。结论 病程7 d以细胞因子TNF-α、IFN-γ、IL-2升高为主，病程7～14 d以细胞因子IL-4、IL-6升高为主，提示细胞因子在MPP的发展过程中有重要意义。

关键词: 肺炎, 支原体, 炎症, 支气管镜检查, 白细胞介素类, 儿童

Abstract: Objective To study the cytokine levels of TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10 in bronchoalveolar lavage fluid (BALF) in children with mycoplasma pneumoniae pneumonia (MPP). Methods Clinical data of 90 patients with MPP (MPP group), who were admitted to Tianjin Children’s Hospital from January to December 2017, were collected in this study. The 90 cases were divided into group A1 (< 7 days), group A2 (7-14 d) and group A3 (15-30 d) according to the course of disease on admission, with 30 cases in each group. Children who were without MPP and needed bronchoscopy examination or treatment at the same time were used as the control group (B group). The expression levels of cytokines (TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10) were detected by enzyme-linked immunoassay (ELISA). Results The serum and BALF levels of TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10 increased significantly in A group compared with those in B group (P < 0.05). The highest levels of cytokines (TNF-α, IFN-γ and IL-2) were found in Group A1. The highest levels of cytokines (IL-4 and IL-6) were found in Group A2. Conclusion The levels of TNF-α, IFN-γ and IL-2 are increased significantly in the disease course of < 7 days. In the disease course of 7 to 14 days, the IL-4 and IL-6 are elevated significantly. Our data suggest that these cytokines may be involved in the pathogenesis of MPP.

Key words: pneumonia, mycoplasma, inflammation, bronchoscopy, interleukins, child