Abstract: Abstract:Objective To investigate the differential expressions of serum proteins by proteomics approach in rat model of sleep deprivation. Methods A rat model of 24-h rapid eye movement sleep deprivation was induced by MMPM. Twenty four rats were randomly and averagely divided into three groups, namely, model group (M), model control group (MC) and blank control group (BC). Changes of body mass in rats were observed. Morris water maze test was used to evaluate the effect of sleep deprivation on learning and memory ability. Serum proteins were separated by two-dimensional electrophoresis and identified by LC-MS/MS. Results There was no significant difference in rat body weight between BC group and MC group. After sleep deprivation, mental irritable, pelage dull and weight loss were found in M group, but no significant changes were found in learning and memory ability. There were no significant differences in the number of protein spots between three groups. Four proteins were down regulated: Serotransferrin, Glutathione peroxidase 3, Ig kappa chain C region, B allele and Collagen alpha- 2(I) chain. Conclusion The short term sleep deprivation may be related to iron metabolism, oxidative d

Key words: sleep deprivation, serum, memory, learning, proteomics, rats, Wistar