Abstract: Abstract: Objective To investigate the changes and roles of the Long non-coding RNA during the reprogramming of human induced pluripotent stem cells. Methods Using Agilent Human lncRNA (4 * 180K) chip to check the of lncRNA in somatic cells, induced pluripotent stem cells and embryonic stem cells. Compared with differentially expressed lncRNA in somatic cells and induced pluripotent stem cells, lncRNA may play an important role during the reprogramming of human pluripotent stem cells. Results The lncRNA profiles in induced pluripotent stem cells are similar to embryonic stem cells but are different from the somatic cells． 3156 differentially expressed lncRNAs are found between stem cells and somatic cells by cluster analysis. 222 differentially expressed lncRNAs are found between during the reprogramming process of human pluripotent stem cells by biological analysis. Conclusion lncRNA may play an important role in human pluripotent stem cell reprogramming process.

Key words: Long non-coding RNA, human pluripotent stem cell, reprogramming, Arrary