Influences of lncRNA SNHG11 on proliferation, apoptosis, migration and invasion of ovarian cancer cells by regulating miR-184/CARM1 signaling axis

doi:10.11958/20221256

Abstract

Abstract:

Objective To investigate the effect of long chain non coding RNA nucleolar small RNA host gene 11 (lncRNA SNHG11) targeting miR-184/CARM1 signal axis on ovarian cancer cells and its mechanism. Methods Expression levels of lncRNA SNHG11 and miR-184 in ovarian cancer tissue and cells were detected by real time fluorescent quantitative PCR (qPCR), and SKOV3 cells were selected for the experiment. SKOV3 cells were divided into the si-NC group (transfected with si-NC), the si-SNHG11 group (transfected with si-SNHG11), the si-SNHG11+anti-NC group (transfected with si-SNHG11+anti-NC), the si-SNHG11+anti-miR-184 group (transfected with si-SNHG11+anti-miR-184), the miR-NC group (transfected with miR-NC), the miR-184 mimics group (transfected with miR-184 mimics), the miR-184 mimics+pcDNA group (transfected with miR-184 mimics+pcDNA) and the miR-184 mimics+CARM1 group (transfected with miR-184 mimics+pcDNA-CARM1). The cell proliferation, apoptosis, migration, invasion and the expression levels of CARM1, E-cadherin, N-cadherin protein in each group were detected respectively. The tumorigenicity of each group of cells in vivo was detected by nude mouse tumorigenesis experiment. The targeting relationship between miR-184 and lncRNA SNHG11, CARM1 was detected by double luciferase reporter gene experiment. Results The expression level of lncRNA SNHG11 was increased in ovarian cancer tissue and cells, and the expression level of miR-184 was decreased in ovarian cancer cells (P<0.05). Compared with the si-NC group, the proliferation, migration and invasion ability of SKOV3 cells were decreased in the si-SNHG11 group, and the tumor weight and volume were decreased, the expression of N-cadherin was decreased, and the apoptosis rate and the expression of E-cadherin protein were increased (P<0.05). Compared with the si-SNHG11+anti-NC group, the proliferation, migration and invasion ability of SKOV3 cells were increased in the si-SNHG11+anti-miR-184 group, the tumor weight and volume were increased, and the expression of N-cadherin protein was increased, and the apoptosis rate and E-cadherin protein expression were decreased (P<0.05). Compared with the miR-NC group, the proliferation, migration and invasion ability of SKOV3 cells were decreased in the miR-184 mimics group, the tumor weight and volume were decreased, the expression of N-cadherin protein was decreased, while the apoptosis rate and expression level of E-cadherin protein were increased (P<0.05). Compared with the miR-184 mimics+pcDNA group, the proliferation, migration and invasion ability of SKOV3 cells were increased in the miR-184mimics+CARM1 group. The tumor weight and volume were increased, and the expression of N-cadherin protein was increased, while the apoptosis rate and E-cadherin protein expression were decreased (P<0.05). LncRNA SNHG11 targetly regulated miR-184/CARM1 axis. Conclusion LncRNA SNHG11 promotes the growth of ovarian cancer by regulating miR-184/CARM1 signal axis.

Key words: ovarian neoplasms, neoplasm invasiveness, genetic therapy, long non-coding RNA small nucleolar RNA host gene 11, microRNA-184, coactivator-associated arginine methyltransferase 1

CLC Number:

R737.31

Figures/Tables 14

References 17

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细胞系	LncRNA SNHG11	miR-184
IOSE80	1.01±0.10	1.02±0.13
Hey	1.66±0.32^a	0.71±0.08^a
ES2	2.23±0.37^a	0.47±0.05^ab
SKOV3	3.25±0.54^abc	0.23±0.04^abc
A2780	1.93±0.41^ad	0.52±0.06^abd
F	28.717^＊＊	84.242^＊＊

细胞系	LncRNA SNHG11	miR-184
IOSE80	1.01±0.10	1.02±0.13
Hey	1.66±0.32^a	0.71±0.08^a
ES2	2.23±0.37^a	0.47±0.05^ab
SKOV3	3.25±0.54^abc	0.23±0.04^abc
A2780	1.93±0.41^ad	0.52±0.06^abd
F	28.717^＊＊	84.242^＊＊

组别	细胞凋亡率（%）	迁移细胞数（个/视野）	侵袭细胞数（个/视野）	E-cadherin	N-cadherin	瘤体质量（g）	瘤体体积（mm³）
si-NC组	2.43±0.78	145.27±8.31	126.53±7.23	0.21±0.04	1.13±0.12	1.53±0.32	1 258.62±35.46
si-SNHG11组	25.32±4.61^a	68.33±4.68^a	54.37±4.36^a	0.87±0.08^a	0.51±0.07^a	0.81±0.25^a	423.68±20.46^a
si-SNHG11+anti-NC组	23.65±3.82	65.27±4.75	57.70±5.21	0.83±0.09	0.47±0.08	0.87±0.27	411.32±18.42
si-SNHG11+anti-miR-184组	12.47±2.66^bc	123.63±8.47^bc	106.43±6.13^bc	0.37±0.05^bc	1.01±0.10^bc	1.33±0.28^bc	734.45±26.23^bc
F	62.853^＊＊	207.937^＊＊	227.120^＊＊	140.731^＊＊	77.176^＊＊	9.359^＊＊	1 398.970^＊＊

组别	细胞凋亡率（%）	迁移细胞数（个/视野）	侵袭细胞数（个/视野）	E-cadherin	N-cadherin	瘤体质量（g）	瘤体体积（mm³）
si-NC组	2.43±0.78	145.27±8.31	126.53±7.23	0.21±0.04	1.13±0.12	1.53±0.32	1 258.62±35.46
si-SNHG11组	25.32±4.61^a	68.33±4.68^a	54.37±4.36^a	0.87±0.08^a	0.51±0.07^a	0.81±0.25^a	423.68±20.46^a
si-SNHG11+anti-NC组	23.65±3.82	65.27±4.75	57.70±5.21	0.83±0.09	0.47±0.08	0.87±0.27	411.32±18.42
si-SNHG11+anti-miR-184组	12.47±2.66^bc	123.63±8.47^bc	106.43±6.13^bc	0.37±0.05^bc	1.01±0.10^bc	1.33±0.28^bc	734.45±26.23^bc
F	62.853^＊＊	207.937^＊＊	227.120^＊＊	140.731^＊＊	77.176^＊＊	9.359^＊＊	1 398.970^＊＊

组别	LncRNA SNHG11	miR-184
si-NC组	1.00±0.10	1.01±0.11
si-SNHG11组	0.41±0.06^a	1.67±0.23^a
si-SNHG11+anti-NC组	0.43±0.07	1.72±0.25
si-SNHG11+anti-miR-184组	0.45±0.06	1.05±0.12^bc
F	88.498^＊＊	25.047^＊＊