Expression and clinical significance of ubiquitin in patients with bicuspid aortic valve

doi:10.11958/20221322

Abstract

Abstract:

Objective To analyze the expression of ubiquitin in patients with bicuspid aortic valve (BAV) and tricuspid aortic valve, and to explore the correlation between ubiquitin and BAV. Methods Fifteen patients with BAV were collected, and 15 cases with tricuspid aortic valve were used as controls. The protein and mRNA expression of ubiquitin in valves of the two groups were examined by Western blot assay and real-time fluorescence quantitative PCR. Results Western blot assay indicated that there was no positive signal band in valve tissue in patients of the two groups. However, the mRNA expression of ubiquitin was found in valve of patients with BAV and tricuspid aortic valve. The mRNA expression of ubiquitin was significantly lower in patients with BAV than that of the control group (P<0.01). Conclusion Ubiquitin mRNA expression is significantly down-regulated in BAV, which suggests that ubiquitin might play a role in the development and progression of BAV.

Key words: ubiquitin, aortic diseases, aortic valve insufficiency, bicuspid aortic valve, tricuspid aortic valve

CLC Number:

R654.27

Figures/Tables 1

References 19

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