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15 September 2023, Volume 51 Issue 9 Previous Issue    Next Issue
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Recommendations and Consensus
Cell and Molecular Biology
Experimental Research
Clinical Research
Applied Research
Review


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Recommendations and Consensus

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Expert consensus on anti-osteoporosis therapy and medication-related jaw necrosis in Tianjin Osteoporosis and Bone Mineral Disease Branch of Tianjin Medical Association, Society of Oral and Maxillofacial Surgery of Tianjin Stomatological Association 2023, 51 (9):  897-903.  doi: 10.11958/20230147 Abstract ( 384 )   HTML ( 14 )   PDF (774KB) ( 649 )   Osteoporosis (OP) and oral diseases are common health problems of the elderly. Recently, the risk of jaw necrosis with anti-OP medication has been increasingly concerned by relevant specialists. In order to avoid or reduce the incidence of medication-related jaw necrosis before and during OP treatment, it is necessary to properly deal with the relationship between oral invasive procedures and anti-OP treatment. It is conducted by the Osteoporosis and Bone Mineral Salt Disease Branch of Tianjin Medical Association and Society of Oral and Maxillofacial Surgery of Tianjin Stomatological Association. After full exchange and communication between doctors engaged in OP treatment and stomatological doctors, clinical consensus are reached on the basis of evidence-based medicine. It is clear that OP and OP medication-related necrosis of jaw are both diseases with serious adverse consequences, and standard OP treatment can reduce fractures and improve the quality of life of elderly. During the treatment period, appropriate measures should be taken to avoid the occurrence of OP medication-related jaw necrosis. Through the joint efforts of doctors from the two disciplines, we hope to solve clinical problems that affect oral operation due to the use of OP therapeutic drugs, and promote and protect the bone health and dental health of elderly. Figures and Tables | References | Related Articles | Metrics

Cell and Molecular Biology

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Tumor suppressor gene SASH1 binds Vimentin and negatively regulates the expression of Vimentin in breast cancer YANG Pingping, ZHANG Jing, CHEN Hongyu, ZHANG Miao, ZHOU Dingan, FANG Wen 2023, 51 (9):  904-908.  doi: 10.11958/20222057 Abstract ( 354 )   HTML ( 14 )   PDF (1325KB) ( 606 )   Objective To investigate the partner molecules that regulate the tumor suppressor gene SASH1 in the occurrence and development of breast cancer, and to analyze the correlation and role of them in breast cancer development. Methods Liquid chromatography tandem mass spectrometry (LC-MS/MS) technology, bioinformatics and immunoprecipitation were used to analyze and confirm molecules that may bind with SASH1 and potentially regulate the occurrence and development of breast cancer. Immunohistochemistry and Western blot assay were used to detect the expression levels of SASH1 and Vimentin in normal breast tissue and breast cancer tissue in humans, and to analyze the correlation between the two in breast cancer. The effect of knocking down SASH1 on the expression of Vimentin was also examined in breast cancer cells using Western blot assay. Results LC-MS/MS technology combined with bioinformatics showed that SASH1 was more likely to bind to Vimentin. Immunoprecipitation indicated that SASH1 could bind to Vimentin. In the immunohistochemical staining of 30 cases of normal breast tissue and 123 cases of breast cancer tissue, it was found that compared with normal breast tissue, SASH1 expression was decreased and Vimentin expression was increased in breast cancer tissue. Furthermore, a negative correlation was observed between the immunohistochemical scores of the two proteins (P<0.01). Western blot assay indicated that in 16 cases of breast cancer tissue and matched adjacent tissue, the breast cancer tissue showed low expression of SASH1 and high expression of Vimentin. After knocking down SASH1 expression in breast cancer cells, the expression of Vimentin increased. Conclusion The tumor suppressor protein SASH1 may participate in the occurrence and development of breast cancer by negatively regulating the expression of Vimentin. Figures and Tables | References | Related Articles | Metrics

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Study on the mechanism of CHD4 regulating telomere function to promote cervical cancer HeLa cell proliferation WANG Qianqian, LI Tingfang, WANG Feng 2023, 51 (9):  909-914.  doi: 10.11958/20222091 Abstract ( 377 )   HTML ( 14 )   PDF (1223KB) ( 569 )   Objective To investigate the effect of chromodomain helicase DNA-binding protein 4 (CHD4) on the proliferation and migration of cervical cancer HeLa cells by regulating telomeres function. Methods CHD4-depleted HeLa cell lines were constructed by lentivirus infection. The mRNA and protein expression levels of CHD4 were detected by real-time fluorescence quantitative PCR (qPCR) and Western blot assay. Cervical cancer HeLa cells were divided into the control group, the shCDH4-1 group and the shCHD4-2 group. The effect of CHD4 on cell proliferation of HeLa cells was detected by CCK-8 assay. The colony formation assay was performed to detect the number of cell colonies. Scratch-healing assay was performed to detect cell migration. In vivo, the tumor formation experiment was used to observe the growth changes of xenograft in nude mice. Immunofluorescence-fluorescence in situ hybridization was performed to detect the co-localization of telomeres and CHD4 in HeLa cells, and the level of damage at telomeres in each group. Telomere damage was indicated by the co-localization of damage factor γH2AX with telomeres. Metaphase-telomere fluorescence in situ hybridization was performed to detect changes in telomere function in each group, and the increased proportion of chromosomes with telomere signal deletion (SFE) or multiple telomere signals (MTS) represented telomere dysfunction. Results HeLa cell lines with stable down-regulated CHD4 were successfully constructed after lentiviral infection (P<0.05). Compared with the control group, the cell proliferation ability, colony formation ability, migration ability and tumor growth ability in vivo were significantly decreased in the shCHD4-1 group and the shCHD4-2 group (P<0.05). Immunofluorescence-fluorescence in situ hybridization assay showed that CHD4 localized to telomeres of HeLa cells. Compared with the control group, the deletion of CHD4 was insufficient to cause DNA damage at telomeres (P>0.05). However, the proportion of SFE chromosomes in HeLa cells increased significantly (P<0.05). Conclusion CHD4 can promote the proliferation and migration of HeLa cells by regulating telomere function. Figures and Tables | References | Related Articles | Metrics

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Expression of CCL18 in glioblastoma and its effect on proliferation and migration of human U87MG cells HUANG Guanyou, GE Xuecheng, GAN Hongchuan, HAO Shuyu, WU Zhen 2023, 51 (9):  915-921.  doi: 10.11958/20221791 Abstract ( 443 )   HTML ( 9 )   PDF (1780KB) ( 595 )   Objective To analyze the expression chemokine (C-C motif) ligand 18 (CCL18) on the clinical prognosis of glioblastoma (GBM) and its effects on the proliferation and migration of U87MG cells. Methods The differential expression of CCL18 gene in GBM and normal brain tissue was analyzed based on the GBM data from Cancer Genome Atlas (TGGA) database and the normal brain tissue data from Genotype-Tissue Expression (GTEx) database. Immunohistochemical staining was used to verification. Kaplan-Meier survival analysis, univariate and multivariable Cox regression analysis were used to evaluate the impact of CCL18 expression on the prognosis in GBM patients. A nomogram was established based on the results of multivariate Cox analysis. The calibration curve and receiver-operating characteristic (ROC) curve were draw for validation. Human glioblastoma U87MG cells were divided into the siRNA-CCL18 group, the siRNA-NC group and the Mock-siRNA group. qPCR was used to detect the content of CCL18 mRNA in the different U87MG cell groups. The CCK-8 assay and cell scratch test were used to detect the cell proliferation and migration ability of U87MG cells in each group. Results CCL18 was significantly upregulated in GBM tissue (P<0.05). The immunohistochemistry results suggested that CCL18 protein was significantly increased in GBM tissue (P<0.05). Survival analysis indicated that high expression of CCL18 was associated with poor prognosis in GBM patients (P<0.05). Multivariate Cox regression analysis revealed that IDH wild-type and high level of CCL18 expression were the two major risk factors affecting the poor prognosis of GBM (P<0.05). The calibration curve showed that the actual survival rate of patients was consistent with the predicted survival rate. The ROC curve demonstrated that the model had a good predictive for predicting the survival of GBM patients. siRNA reduced the expression of CCL18 in human U87MG cells. The results of CCK-8 assay showed that the proliferation of U87MG cells was significantly inhibited (P<0.05), and the wound healing scratch ability of U87MG cells with low expression of CCL18 decreased (P<0.01). Conclusion CCL18 is over-expressed in GBM tissue, and high CCL18 expression is associated with poor prognosis in GBM patients．CCL18 promotes GBM cell proliferation and migration and may act as a relevant predictive biomarker for GBM. Figures and Tables | References | Related Articles | Metrics

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Effects of aconitine regulating miR-181d-5p/DDX3 axis on proliferation and apoptosis of HeLa cells of cervical cancer ZHAO Dandan, ZHANG Sue, MIAO Liye, WANG Yan 2023, 51 (9):  922-927.  doi: 10.11958/20221825 Abstract ( 285 )   HTML ( 11 )   PDF (1191KB) ( 591 )   Objective To investigate effects of aconitine on proliferation and apoptosis of cervical cancer HeLa cells by regulating the microRNA-181d-5p (miR-181d-5p)/DEAD-box RNA helicase 3 (DDX3) axis. Methods Cervical cancer HeLa cells were treated with different doses of aconitine, and cell proliferation was detected by tetramethylazolium salt method to determine the dose. HeLa cells were divided into the control group (normal culture, no treatment), the aconitine low dose group (4 mg/L), the aconitine medium dose group (8 mg/L), the aconitine high dose group (16 mg/L), the cisplatin group (5 mg/L), the aconitine high dose + miR-NC group [16 mg/L aconitine + transfected with miR-181d-5p siRNA negative control (miR-NC) plasmid] and the aconitine high dose + miR-181d-5p low expression group [16 mg/L aconitine + transfected miR-181d-5p small interfering RNA (siRNA) plasmid]. Plate clone formation experiment and flow cytometry were used to detect the clonal formation number and apoptosis of HeLa cells in each group. Expression levels of miR-181d-5p and DDX3 messenger RNA (mRNA) in HeLa cells were detected by real-time fluorescent quantitative PCR. Western blot assay was used to detect expression levels of DDX3, Cyclin D1, proliferating cell nuclear antigen (PCNA), B cell lymphocytoma-2 (Bcl-2), Bcl-2 related X protein (Bax) and Caspase-3 protein in HeLa cells. Dual luciferase reporter gene experiment was used to verify the targeting relationship between miR-181d-5p and DDX3. Results HeLa cells were treated with 0.5-64 mg/L aconitine for 24 h, 48 h and 72 h, respectively, and HeLa cells were inhibited to varying degrees. Aconitine doses of 4 mg/L, 8 mg/L and 16 mg/L were selected for follow-up experiments. Compared with the control group, HeLa cell clonal formation number, DDX3 mRNA and protein, Cyclin D1, PCNA and Bcl-2 protein expression levels were decreased successively in the aconitine low, medium and high dose groups and the cisplatin groups (P<0.05), and the apoptosis rate, expression levels of miR-181d-5p, Bax and Caspase-3 protein were increased successively (P<0.05). Compared with the aconitine high dose group and the aconitine high dose + miR-NC group, HeLa cell clonal formation number, DDX3 mRNA and protein, Cyclin D1, PCNA and Bcl-2 protein expression levels were increased in the aconitine high dose + miR-181d-5p low expression group (P<0.05), and the apoptosis rate, expression levels of miR-181d-5p, Bax and Caspase-3 protein were decreased (P<0.05). Dual luciferase reporter gene detection confirmed the targeting relationship between miR-181d-5p and DDX3. Conclusion Aconitine can regulate the miR-181d-5p/DDX3 axis, promote the expression of miR-181d-5p, inhibit the expression of DDX3, and then inhibit the proliferation of cervical cancer HeLa cells and promote cell apoptosis. Figures and Tables | References | Related Articles | Metrics

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MiR-155 affects the biological functions of trophoblastic cells through regulating cGMP-dependent kinase 1 and is involved in the mechanism of preeclampsia YUE Jinjing, ZENG Ying, GUO Xiaopei, DONG Yue, JI Ruonan, PENG Rui, LUO Xiaohua 2023, 51 (9):  928-934.  doi: 10.11958/20221869 Abstract ( 436 )   HTML ( 8 )   PDF (1207KB) ( 581 )   Objective To investigate the expression levels of miR-155 and cGMP-dependent protein kinase 1 (PKG1) in placental tissue of patients with preeclampsia, and the effect of miR-155 on the function of trophoblasts HTR-8/SVneo by inhibiting PKG1 under the mediation of nucleus factor kappa B (NF-κB). Methods Twenty placentas were collected from normal pregnant women and pre-eclampsia pregnant women who delivered by cesarean section. In vitro trophoblasts HTR-8/SVneo were cultured and divided into the NC group, the mimics group, the inhibitor group, the siRNA NC group, the PKG1 siRNA group and the siRNA+inhibitor group. Cells were treated with NF-κB inhibitor PDTC and divided into the control group, the PDTC group, the PDTC+NC group and the PDTC+mimics group. Real-time quantitative polymerase chain reaction (qPCR) was performed to detect the expression of miR-155 and PKG1 mRNA in placentas and HTR-8/SVneo cells. Western blot assay was performed to measure the level of PKG1 protein. The cell proliferation, migration and apoptosis were assessed by CCK-8 assay, Transwell assay and flow cytometry. Results The expression of miR-155 was significantly upregulated in placental tissue of the PE group, while the expression of PKG1 decreased significantly (P<0.05). The vitro experiments showed that compared with the NC group, the expression of PKG1 was significantly reduced in the miR-155 mimics group (P<0.05). The migration and proliferation ability of trophoblast was significantly weakened, the apoptotic ability was significantly enhanced (P<0.05). The expression of PKG1 was significantly increased in the miR-155 inhibitor group, the migration and proliferation ability of trophoblast was significantly enhanced, and the apoptotic ability was significantly weakened. Compared with the control group, the expression of miR-155 was significantly reduced in the PDTC group, the migration and proliferation ability of trophoblast was significantly enhanced, and the apoptotic ability was significantly weakened (P<0.05). Conclusion Results indicate that the expression of miR-155 is upregulated in preeclampsia, and can affect proliferation, migration and apoptosis of trophoblast cells by down-regulating PKG1 mediated by NF-κB. Figures and Tables | References | Related Articles | Metrics

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Expression and clinical significance of ubiquitin in patients with bicuspid aortic valve CUI Fenfen, LI Yuanmin 2023, 51 (9):  935-937.  doi: 10.11958/20221322 Abstract ( 274 )   HTML ( 12 )   PDF (770KB) ( 566 )   Objective To analyze the expression of ubiquitin in patients with bicuspid aortic valve (BAV) and tricuspid aortic valve, and to explore the correlation between ubiquitin and BAV. Methods Fifteen patients with BAV were collected, and 15 cases with tricuspid aortic valve were used as controls. The protein and mRNA expression of ubiquitin in valves of the two groups were examined by Western blot assay and real-time fluorescence quantitative PCR. Results Western blot assay indicated that there was no positive signal band in valve tissue in patients of the two groups. However, the mRNA expression of ubiquitin was found in valve of patients with BAV and tricuspid aortic valve. The mRNA expression of ubiquitin was significantly lower in patients with BAV than that of the control group (P<0.01). Conclusion Ubiquitin mRNA expression is significantly down-regulated in BAV, which suggests that ubiquitin might play a role in the development and progression of BAV. Figures and Tables | References | Related Articles | Metrics

Experimental Research

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A study of information transmission in HPC-PFC network during working memory encoding induced by propofol anesthesia GUO Dongyong, LI Baoling, BAI Wenwen, LIU Tiaotiao, XU Xinyu 2023, 51 (9):  938-942.  doi: 10.11958/20230307 Abstract ( 308 )   HTML ( 9 )   PDF (1253KB) ( 569 )   Objective To figure out whether propofol anesthesia impairs information transmission from hippocampal to prefrontal cortex (HPC-PFC) neural pathways during the coding phase of working memory in rats. Methods Among 12 rats, 6 SD rats (3 months old) were selected for research analysis, and 16-channel microelectrode arrays were implanted into the medial prefrontal cortex (mPFC) and the ventral hippocampus (vHPC) of rats, respectively. The Cerebus information acquisition system was used to record the working memory coding stage tasks of each rat before and 12 and 24 hours after anesthesia with 150 mg/kg propofol. The multichannel local field potential (LFPs) signals of mPFC and vHPC, two responsible brain regions, were used to establish the vHPC-mFPC network. The directional transfer function (DTF) and the information flow of vHPC-mPFC neural pathway in vHPC and mPFC networks were calculated respectively, and the information transmission of vHPC-mPFC neural pathway before and after anesthesia was quantitatively characterized. Results During working memory encoding, information flow from vHPC to mPFC was significantly reduced 12 h after propofol anesthesia comparison with before propofol anesthesia, but it recovered at 24 h after propofol injection. There were no significant differences in the average vHPC and mPFC network connection strength and vHPC-mPFC information flow of rats between before anesthesia and 24 h after anesthesia. Conclusion These results indicated that working memory impairment induced by propofol may result from disrupting information transmission in vHPC-mPFC network during working memory encoding. Figures and Tables | References | Related Articles | Metrics

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Effect and mechanism of tetrandrine on airway remodeling in bronchial asthma mice WANG Shengcheng, LI Qi, CAI Xiaoyang, TANG Yongjie 2023, 51 (9):  943-947.  doi: 10.11958/20222063 Abstract ( 391 )   HTML ( 10 )   PDF (873KB) ( 574 )   Objective To investigate the effect of tetrandrine on airway remodeling and high mobility group protein box 1 (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway in bronchial asthma mice. Methods A mouse model of bronchial asthma was established, and 40 model mice were randomly divided into the model group, the tetrandrine low dose (20 mg/kg) group, the high dose (40 mg/kg) group and the dexamethasone (10 mg/kg) group, with 10 mice in each group. Another 10 mice without modeling were useed as the control group. Each group was given corresponding intervention for 2 weeks. Serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL-6) and IL-1β were determined by ELISA. Hematoxylin-eosin staining was used to observe lung histopathological changes, and lung injury score was performed. The thickness of bronchial tube wall and bronchial smooth muscle were determined. Expression levels of HMGB1, TLR4, NF-κB mRNA and protein in lung tissue were detected by fluorescence quantitative PCR (qPCR) and Western blot assay. Results No pathological damage was found in bronchus of lung tissue in the control group. Compared with the control group, the bronchial tube wall and smooth muscle of lung tissue in the model group were thickened, mucosal epithelium hyperplasia, and lumen shrank and tracheal mucus exudated significantly. Serum levels of TNF-α, IL-6 and IL-1β, lung injury score, bronchial wall thickness, bronchial smooth muscle thickness, HMGB1, TLR4, NF-κB mRNA and protein expression levels were increased significantly (P<0.05). Compared with the model group, the degree of lung lesions was gradually reduced in the low and high dose tetrandrine groups, and serum levels of TNF-α, IL-6, IL-1β, lung injury scores, bronchial wall and bronchial smooth muscle thickness, HMGB1, TLR4, NF-κB mRNA and protein expression were successively decreased. There were no significant differences in above indexed between the tetrandrine high dose group and the dexamethasone group (P>0.05). Conclusion Tetrandrine can reduce the degree of airway remodeling and the level of inflammatory factors in mice with bronchial asthma, and protect lung tissue, which may be related to the inhibition of HMGB1/TLR4/NF-κB signal pathway. Figures and Tables | References | Related Articles | Metrics

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Effect of curcumin regulating NMDAR/Ca2+/CaMKⅡ signaling pathway on postoperative cognitive dysfunction induced by isoflurane in young mice MA Huimin, LIU Lu, XIONG Ying, ZHAO Hui, KONG Fanli 2023, 51 (9):  948-954.  doi: 10.11958/20221719 Abstract ( 398 )   HTML ( 8 )   PDF (1180KB) ( 568 )   Objective To investigate the effect of curcumin (Cur) regulating N-methyl-D-aspartate receptor (NMDAR)/calcium ion (Ca2+)/calmodulin dependent protein kinase Ⅱ (CaMKⅡ) signaling pathway on isoflurane (ISO) -induced postoperative cognitive dysfunction (POCD) in young mice. Methods Seventy-two C57BL/6J mice were separated into the control group, the ISO group, the low-dose Cur group (Cur-L group, 50 mg/kg), the medium-dose Cur group (Cur-M group, 100 mg/kg), the high-dose Cur group (Cur-H group, 200 mg/kg) and the Cur-H+NMDA (NMDAR activator) group (200 mg/kg+8 mg/kg), with 12 animals in each group. After 30 min of corresponding drug treatment, the mice in the control group inhaled a mixed gas containing 30% oxygen and air for 2 hours, and mice in the other groups inhaled 2% ISO for 2 hours, once a day for 14 days. Twenty-four hours after the last treatment, Morris water maze test was used to detect the learning and spatial memory abilities of mice. HE staining was used to detect the pathological changes in hippocampal CA1 area. Immunofluorescence staining was used to detect the positive expression of neuron specific nucleoprotein (NeuN) in hippocampus CA1 region of mice. TUNEL staining was used to detect neuronal apoptosis. Enzyme linked immunosorbent assay was used to detect levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in hippocampal CA1 tissue. Western blot assay was used to detect NMDAR1 and CaMKⅡ protein expression in mouse hippocampal CA1 tissue. Intracellular Ca2+ concentration in hippocampal CA1 region was detected by fluorescence probe. Results Compared with the control group, the pathological damage of hippocampal CA1 region of mice was severe in the ISO group, and the escape latency was prolonged. The apoptosis rate of neural cells, levels of IL-1β and TNF-α, expression levels of NMDAR1 and CaMKⅡ protein, and the concentration of Ca2+ in hippocampal CA1 region were increased (P<0.05). Times of crossing platform and the number of NeuN positive cells were decreased in the ISO group (P<0.05). Compared with the ISO group, pathological damages of hippocampal CA1 region of mice were alleviated in the Cur-L group, the Cur-M group and the Cur-H group, the escape latency was shortened, and the apoptosis rate of neural cells, levels of IL-1β and TNF-α, expression levels of NMDAR1 and CaMKⅡ protein, and the concentration of Ca2+ in hippocampal CA1 region were decreased (P<0.05). Times of crossing platform and the number of NeuN positive cells were increased (P<0.05), which was dose-dependent. NMDA attenuated the improvement effect of high-dose Cur on ISO induced POCD in mice (P<0.05). Conclusion Curcumin may improve ISO-induced POCD in mice by inhibiting NMDAR/Ca2+/CaMKⅡ signaling pathway. Figures and Tables | References | Related Articles | Metrics

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Effects of Mongolian astragalus saponin on neuroinflammation and intestinal microflora disorder induced by lead exposure in developing rats HU Mingyue, LI Xin, GAO Lei, GUAN Mingjie 2023, 51 (9):  955-960.  doi: 10.11958/20221821 Abstract ( 375 )   HTML ( 9 )   PDF (1191KB) ( 591 )   Objective To investigate the effect of Mongolian astragalus saponin on neuroinflammation and intestinal microbiota disorder caused by lead exposure in developing SD rats. Methods Forty 4-week-old Sprague Dawley rats were randomly divided into the control group (CG), the lead exposure group (LE) and the saponin intervention groups [high-dose (SH), medium-dose (SM) and low-dose (SL)]. After 4 weeks of corresponding intervention, blood lead content of rats was detected by hydride generation atomic fluorescence spectrometry. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in hippocampal tissue were detected by enzyme linked immunosorbent assay. Changes of intestinal microbiota composition in cecal contents were detected by 16S rDNA sequencing. Results Compared with the CG group, blood lead levels were increased in other groups(P<0.05), levels of TNF-α, IL-6 and IL-1β in hippocampus were increased in the LE group, and the α and β diversity of intestinal flora were significantly changed in the LE group. Shannon index and Simpson index were decreased, and relative abundance of Romboutsia, Blautia and Lachnospiraceae_UCG-001 was decreased. The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 was increased (P<0.05). Compared with the LE group, blood lead levels were decreased in the SL group and the SM group (P<0.05). Hippocampal IL-6 level in the SL group, the level of IL-1β in the SM group, levels of TNF-α and IL-6 in the SM group and the SH group were decreased (P<0.05). β diversity in the SL group and the SM group, and α diversity in the SM group were significantly improved, and Simpson index was increased in the SM group (P<0.05). The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 decreased in the SM group and the SH group (P<0.05). The relative abundance of Romboutsia, Lachnospiraceae_UCG-001 and Blautia were significantly increased in the SM group and the SL group (P<0.05). The abundance of Romboutsia increased more significantly in the SM group than that in the SH group (P<0.05). Conclusion Mongolian astragalus saponins can effectively inhibit neuroinflammatory response and intestinal flora disorder in hippocampus of lead-induced developing rats, and the effect of medium dose intervention is obvious. Figures and Tables | References | Related Articles | Metrics

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Mechanism of Shujin Huoxue Capsule in relieving knee osteoarthritis in rats through JAK2/STAT3 pathway LI Hongjun, QIAN Liang, DENG Xinchao, YU Ting, WU Yan, WU Hongli 2023, 51 (9):  961-967.  doi: 10.11958/20221924 Abstract ( 450 )   HTML ( 12 )   PDF (1677KB) ( 640 )   Objective To investigate the effect and mechanism of Shujin Huoxue Capsule on knee osteoarthritis (KOA) in rats by regulating tyrosine protein kinase 2 (JAK2)/signal transduction and transcription-activating protein 3 (STAT3) signaling pathway. Methods The Hulth method was used to prepare rat KOA model. Sixty rats were divided into the Sham group, the KOA group, the Shujin Huoxue Capsule (SJHX) group (472.5 mg/kg Shujin Huoxue Capsule gavage) and the AG490 group (5.0 mg/kg JAK2 inhibitor AG490 intraperitoneally) with 15 rats in each group. The pathological changes of synovial tissue and cartilage tissue were observed by HE staining and Safranin O-fast green staining. Serum levels of interleukin-10 (IL-10), IL-1β and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). M1/M2 macrophage polarization was detected by immunofluorescence assay. The expression levels of IL-1β, inducible nitric oxide synthase (iNOS) and matrix metalloproteinase 13 (MMP-13) were detected by immunohistochemical staining. JAK2/STAT3 pathway related proteins were detected by Western blot assay. Results Compared with the Sham group, the articular surface was uneven in rats of the KOA group. The synovial tissue and cartilage tissue were destroyed, pathological scores were increased. Serum levels of IL-1β and TNF-α were obviously increased, and the level of IL-10 was obviously reduced. Expression levels of IL-1β, iNOS, MMP-13, p-JAK2/JAK2 and p-STAT3/STAT3 in synovial tissue, and the proportions of M1, M2 macrophages and M1/M2 were obviously increased (P<0.05). Compared with the KOA group, the pathological damage of synovial tissue and cartilage tissue was reduced in the SJHX group and the AG490 group. Pathological scores were reduced. Serum levels of IL-1β and TNF-α were obviously reduced, and the level of IL-10 was obviously increased. Expression levels of IL-1β, iNOS, MMP-13, p-JAK2/JAK2, p-STAT3/STAT3 in synovial tissue, and the proportions of M1, M2 macrophages and M1/M2 were obviously reduced (P<0.05). Conclusion Shujin Huoxue Capsule can improve the symptoms of synovitis in KOA rats by inhibiting the activation of JAK2/STAT3 signaling pathway and regulating the polarization of M1/M2 macrophages. Figures and Tables | References | Related Articles | Metrics

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Effects of mild hypothermia treatment on coagulation function and inflammatory factors in a swine model after cardiac arrest and extracorporeal cardiopulmonary resuscitation WANG Jinxiang, XU Guowu, JIN Heng, HE Bin, CHAI Yanfen 2023, 51 (9):  968-971.  doi: 10.11958/20230001 Abstract ( 401 )   HTML ( 7 )   PDF (761KB) ( 601 )   Objective To investigate effects of mild hypothermia treatment on coagulation function and inflammatory factors in a swine model of extracorporeal cardiopulmonary resuscitation (ECPR) after cardiac arrest (CA). Methods Using random number table method, twelve Bama pigs were divided into the control group, the normal temperature group and the mild hypothermia group with four pigs in each group. CA model was established by right ventricular fibrillation in the normal temperature group and the mild hypothermia group, and then ECPR was performed by extracorporeal membrane oxygenation (ECMO). The target temperature of the normal temperature group was 37 ℃, and the mild hypothermia group was 34 ℃. Control group was only given conventional tube placement. Pigs were killed 24 hours later, and venous blood was taken to detect levels of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB) and D-dimer antigen (D-D). Expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme linked immunosorbent assay (ELISA). Results Compared with the control group, PT, APTT, TT, FIB, D-D and expression levels of TNF-α and IL-6 were significantly increased in the normal temperature group and the mild hypothermia group. Compared with the normal temperature group, the above indicators were decreased significantly in the mild hypothermia group (P<0.05). Conclusion Mild hypothermia treatment can significantly improve coagulation function and reduce the release of inflammatory factors in a swine model of ECPR after CA, so as to alleviate the occurrence of ECPR-related complications and inflammatory reactions. Figures and Tables | References | Related Articles | Metrics

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Butyrate reduces blood pressure in hypertensive rats by activating the G protein-coupled receptor 41/43 pathway QIN Chundi, MA Wen, LI Yuan, ZHU Yaquan, LI Yu, ZOU Lin, ZHANG Xin 2023, 51 (9):  972-976.  doi: 10.11958/20221713 Abstract ( 378 )   HTML ( 12 )   PDF (879KB) ( 584 )   Objective To investigate the mechanism of butyrate reducing blood pressure in hypertensive model rats by activating G protein-coupled receptor 41/43 (GPR41/43) pathway. Methods Seventy-five male SD rats were randomly divided into the sham operation group (n=15) and the model group (n=60). Hypertensive rat model was established by two kidneys and one clip method. Model rats were randomly divided into the control group (0.1 mL/kg ultra-pure water), the butyrate high-dose group (220 mg/kg), the butyrate low-dose group (110 mg/kg) and the valsartan group (30 mg/kg), with 15 rats in each group. Rats were given intervention by cntinuous gavage for 4 weeks. Caudal artery systolic blood pressure (SBP) and heart rate (HR) were measured before and after administration. The serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and endothelial nitric oxide synthase (eNOS) were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression levels of IL-6, TNF-α and eNOS in thoracic aorta were detected by real-time fluorescent quantitative PCR (qPCR). The expression of GPR41/43 in rat thoracic aorta was detected by immunohistochemistry. Results After 2 weeks of administration, SBP was significantly higher in the control group than that in the sham operation group (P<0.05). Values of SBP were significantly lower in the high dose butyrate group, the low dose butyrate group and the valsartan group than those in the control group, and SBP was significantly lower in the butyrate high dose group than that of the butyrate low dose group (P<0.05). After 4 weeks of administration, SBP was significantly lower in the high dose group than that in the low dose group (P<0.05). There were no significant differences in HR before and after administration between groups (P>0.05). After administration, the protein expression and mRNA expression of eNOS were increased in the butyrate high dose group and the butyrate low dose group compared with those of the control group, and the protein expression and mRNA expression of IL-6 and TNF-α were decreased (P<0.05). Immunohistochemistry showed that the expression of GPR41/43 in rat thoracic aorta tissue was increased compared with that of the control group, and which was higher than that in the valsartan group (P<0.05). Conclusion Butyrate has a significant antihypertensive effect on hypertensive rats, which may be related to the activation of GPR41/43 pathway to increase vasodilation and inhibit expression of inflammatory factors.. Figures and Tables | References | Related Articles | Metrics

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The effect of securinine on neurological function recovery after cerebral ischemia-reperfusion injury in rats ZHANG Jinwu, XIE Dingling, CHEN Li 2023, 51 (9):  977-982.  doi: 10.11958/20221801 Abstract ( 321 )   HTML ( 9 )   PDF (1515KB) ( 572 )   Objective To explore the effect and mechanism of securinine (SE) on cerebral ischemia reperfusion injury (CIRI) in rats based on toll-like receptor 4 (TLR4)/nuclear transcription factor-kappa B (NF-κB) pathway. Methods A total of 100 male or female adult SD rats were randomly divided into the sham group, the CIRI group and the SE low-, medium- and high-dose (20 mg/kg, 40 mg/kg, 80 mg/kg) groups, with 20 rats in each group. The rat model of CIRI was established by thread occlusion. Rats were treated with SE (20 mg/kg, 40 mg/kg and 80 mg/kg) immediately after successful modeling for 3 consecutive days. Rats in the sham group and the CIRI group were given the same amount of normal saline. At 24 h, 48 h and 72 h after operation, the neurological deficits of rats were measured according to Longa score. At 72 h after operation, brain tissue water content was evaluated by wet-dry weight method, and the percentage of cerebral infarction volume was assessed by triphenyltetrazolium chloride (TTC) staining. Protein expression levels of Iba-1，TLR4, NF-κB p65 and p-NF-κB p65 in brain tissue were determined by Western blot assay. Expression levels of inflammatory factors such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). Level of microglia activation and the number of surviving neurons were examined by immunofluorescence staining. Results Compared with the sham group, neurological function scores, brain tissue water content and the percentage of cerebral infarction volume were significantly increased in the CIRI group (all P<0.05). Expression levels of TLR4, p-NF-κB p65, Iba-1, IL-1β, TNF-α and IL-6 in brain tissue were significantly increased (all P<0.05). However, medium- and high-doses of SE could significantly alleviate the neurological deficits, reduce the water content of brain tissue and the size of cerebral infarction volume, decrease expression levels of TLR4, p-NF-κB p65, Iba-1, IL-1β, TNF-α and IL-6 in the injured area of brain tissue in rats. Conclusion SE can alleviate CIRI-induced neuroinflammatory response via inhibiting TLR4/NF-κB pathway activation, thereby conferring a protective role in brain of rats. Figures and Tables | References | Related Articles | Metrics

Clinical Research

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The prognostic value of serum sFasL and s-Met levels in patients with sepsis secondary to pneumonia YU Bingchang, LAI Zhenyu, ZHAO Zhanqing, XIE Xiaofang, CAI Qiuyan 2023, 51 (9):  983-987.  doi: 10.11958/20230029 Abstract ( 433 )   HTML ( 8 )   PDF (821KB) ( 558 )   Objective To investigate the prognostic value of serum soluble Fas ligand (sFasL) and soluble interstitial epidermal transforming factor (s-Met) in patients with pneumonia and sepsis. Methods A total of 150 patients with pneumonia secondary sepsis were were divided into the survival group (96 cases) and the death group (54 cases) according to the prognosis of 28 days after admission. Seventy patients with pneumonia were selected as the pneumonia group, and 60 healthy people were selected as the control group. Serum sFasL and s-Met levels were detected by ELISA in all subjects. Spearman rank correlation analysis was used for correlation analysis of sFasL, s-Met levels and SOFA, q-SOFA scores in patients with pneumonia secondary sepsis. Multivariate Logistic regression analysis was used to analyze factors influencing the death of patients with pneumonia secondary sepsis. The diagnostic value of each index in death of patients with pneumonia secondary sepsis was analyzed by receiver characteristic operating curve. Results Levels of sFasL and s-Met increased sequentially immediately after admission in the control group, the pneumonia group and the pneumonia combined with sepsis group (P<0.05). Compared with the survival group, serum sFasL and s-Met levels were higher at 0, 24, 48, 72 and 120 h in the death group (P<0.05). Serum PCT, sFasL, s-Met and q-SOFA score were independent risk factors for death of patients with pneumonia secondary sepsis. The area under the curve of the combined detection of serum PCT, sFasL, s-Met, and q-SOFA scores for predicting mortality in patients with pneumonia secondary sepsis was 0.872 (0.838-0.909), which was higher than the single indicator detection of serum PCT, sFasL, s-Met, and q-SOFA scores of 0.778 (0.739-0.817), 0.795 (0.761-0.829), 0.712 (0.672-0.753) and 0.815 (0.774-0.857) (Z=6.450, 4.305, 5.117, 2.384, all P<0.05). Conclusion The serum sFasL and s-Met levels in patients with pneumonia secondary sepsis are serum biomarkers for evaluating the prognosis of pneumonia secondary sepsis patients. Figures and Tables | References | Related Articles | Metrics

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Changes of serum angiopoietin-like protein 2 and 4 levels and their relationship with ovarian interstitial hemodynamics in patients with PCOS ZHAO Fangyuan, ZOU Hong, SHI Simao, XU Fang, ZUO Dongdong 2023, 51 (9):  988-992.  doi: 10.11958/20222119 Abstract ( 396 )   HTML ( 12 )   PDF (965KB) ( 955 )   Objective To investigate changes of serum Angptl2 and Angptl4 levels in patients with polycystic ovary syndrome (PCOS) and their relationship with ovarian interstitial hemodynamics. Methods A total of 123 PCOS patients (the PCOS group) and 41 healthy volunteers (the control group) were selected. The serum Angptl2 and Angptl4 levels, the peak systolic velocity (PSV), pulsation index (PI) and resistance index (RI) of ovarian interstitial were compared between the two groups. The relationship between Angptl2, Angptl4, PSV, PI, RI and PCOS and their predictive value to PCOS were analyzed. Results The PI and RI values of left and right ovarian interstitial were lower in the PCOS group than those in the control group, and PSV values were higher than those in the control group (P<0.05). Pearson analysis showed that serum Angptl2 and Angptl4 were positively correlated with PSV of left and right ovarian interstitial, and negatively correlated with PI and RI of left and right ovarian interstitial in patients with PCOS (P<0.01). Logistic regression results showed that high HOMA-IR (OR=1.921, 95%CI: 1.017-4.154), high BMI (OR=1.459, 95%CI: 1.085-3.220), high Angptl2 (OR=2.625, 95%CI: 1.330-6.324), high Angptl4 (OR=3.543, 95%CI: 1.915-8.147) levels were related factors affecting the occurrence of PCOS (P<0.05). Receiver operating characteristic curve (ROC) showed that the AUC (95%CI) of predicted PCOS were 0.747 (0.661-0.821) and 0.769 (0.685-0.841) when serum Angptl2 and Angptl4 were used alone. It was lower than that of 0.879 (0.793-0.921, P<0.05) for the combined application. Conclusion The abnormal levels of serum Angptl2 and Angptl4 in PCOS patients are related to the hemodynamics of ovarian interstitial, and they are closely related to the occurrence of PCOS, which can be used as reference indexes for the evaluation of PCOS. Figures and Tables | References | Related Articles | Metrics

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Correlation analysis of circRNA-ZNF532 and circRNA-HIPK3 with diabetic retinopathy SUI Yuan, ZHANG Chengsen, WANG Shuang, LI Xueli, GOU Xiaomei 2023, 51 (9):  993-997.  doi: 10.11958/20230048 Abstract ( 381 )   HTML ( 12 )   PDF (811KB) ( 574 )   Objective To investigate the correlation between circrNa-Zinc finger protein 532 (ZNF532), circrNa-homologous domain interaction protein kinase-3 (HIPK3) and diabetic retinopathy (DR). Methods A total of 109 patients with DR (DR group), 110 patients with simple diabetes (DM group) and 65 healthy volunteers (control group) were selected from our hospital from October 2020 to October 2022. Serum circRNA-ZNF532 and circRNA-HIPK3 expression levels were detected by real-time quantitative polymerase chain reaction (RT-PCR), and serum vascular endothelial growth factor (VEGF), interleukin (IL) -1β, tumor necrosis factor (TNF) -α and IL-6 levels were detected by enzyme-linked immunosoradsorption assay. The correlation between serum circRNA-ZNF532 and circRNA-HIPK3 expression and serum levels of VEGF, IL-1β, TNF-α and IL-6 were analyzed by Pearson method. Univariate Logistic regression analysis and multivariate Logistic regression analysis were used for DR risk factors, and receiver operating characteristic curve (ROC) was used for analysis of circRNA-ZNF532 and circRNA-HIPK3 diagnostic value of DR. Results Serum expressions of circRNA-ZNF532 and circRNA-HIPK3 and levels of VEGF, IL-1β, TNF-α and IL-6 were significantly higher in the DR group than those in the DM group and the control group (P<0.05). Serum circRNA-ZNF532 and circRNA-HIPK3 expression were positively correlated with levels of VEGF, IL-1β, TNF-α and IL-6 in the DR group (P<0.05). Multivariate Logistic regression analysis showed that high levels of VEGF, circRNA-ZNF532 and circRNA-HIPK3 were risk factors for developing DR in diabetic patients (P<0.05). The areas under the curve of circRNA-ZNF532, circRNA-HIPK3 and VEGF in the diagnosis of DR in diabetic patients were 0.736, 0.740 and 0.864. Combined diagnosis was higher than that of circRNA-ZNF532, circRNA-HIPK3 and VEGF alone (P<0.05). Conclusion The up-regulated expression levels of circRNA-ZNF532 and circRNA-HIPK3 in serum of diabetic patients are related to the occurrence of DR, and both circRNA-ZNF532 and circRNA-HIPK3 can be used as potential indicators for the auxiliary diagnosis of DR. Figures and Tables | References | Related Articles | Metrics

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Changes and clinical significance of serum circPVT1 and miR-486-5p levels before and after radiotherapy for advanced NSCLC ZHANG Tianwei, ZHANG Jinbiao, ZHANG Yan, MING Hui, ZHANG Peng, NIE Dong 2023, 51 (9):  998-1001.  doi: 10.11958/20230346 Abstract ( 351 )   HTML ( 11 )   PDF (832KB) ( 867 )   Objective To investigate changes of serum levels of circulating RNA plasmacytoma variant translocation 1 (circPVT1) and microRNA-486-5p (miR-486-5p) before and after radiotherapy in patients with advanced non-small cell lung cancer (NSCLC) and their evaluation value for radiotherapy efficacy. Methods A total of 137 patients with advanced NSCLC were selected as the NSCLC group, and 140 health examination personnel in our hospital were selected as the control group. Real-time fluorescence quantitative PCR (RT-qPCR) was applied to detect serum expression levels of circPVT1 and miR-486-5p. Serum levels of circPVT1 and miR-486-5p before and after radiotherapy were compared. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum circPVT1 and miR-486-5p levels for radiotherapy efficacy. Results Compared with the control group, the serum expression level of circPVT1 was obviously higher before radiotherapy in the NSCLC group, and the expression level of miR-486-5p was obviously lower (P<0.05). According to Targetscan online analysis, circPVT1 had a targeted relationship with miR-486-5p. There were no significant differences in serum expression levels of circPVT1 and miR-486-5p before radiotherapy between patients with squamous cell carcinoma and adenocarcinoma NSCLC (P>0.05). Compared with TNM stage Ⅲ group, the serum expression level of circPVT1 was obviously higher in patients with stage IV NSCLC, and serum expression level of miR-486-5p was obviously lower (P<0.05). The serum expression level of circPVT1 was obviously lower in patients after radiotherapy than that before radiotherapy, and the expression level of miR-486-5p was obviously higher (P<0.05). Compared with the effective radiotherapy group, the serum expression level of circPVT1 was increased and the expression level of miR-486-5p was decreased before and after radiotherapy in the ineffective group (P<0.05). Compared with before radiotherapy, both the effective and ineffective radiotherapy groups showed a decreased serum circPVT1 level and an increased miR-486-5p level after radiotherapy (P<0.05). The AUC (95%CI: 0.859-0.958) of combined diagnosis of serum circPVT1 and miR-486-5p was 0.918, the sensitivity was 80.65% and the specificity was 88.00%. The combined diagnosis was better than single diagnosis (Z=2.06, 2.024, P<0.05). Conclusion Serum levels of circPVT1 and miR-486-5p have certain value in evaluating the efficacy of radiotherapy for advanced NSCLC. Figures and Tables | References | Related Articles | Metrics

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Effect of esketamine on negative postoperative behavioural outcomes after tonsillectomy in preschool children XUE Jianming, SUN Shuo, GUO Yunfei, LI Xiaomin, LI Jianling 2023, 51 (9):  1002-1006.  doi: 10.11958/20221915 Abstract ( 449 )   HTML ( 10 )   PDF (778KB) ( 602 )   Objective To investigate the effect of esketamine on negative postoperative behavioral changes (NPOBCs) in preschool children after tonsillectomy. Methods Seventy eight preschool children with tonsillectomy were selected and divided into the test group and the control group by random number table method, with 39 cases in each group. During anesthesia induction, patients in the test group were intravenously injected with 0.5 mg/kg esketamine. In contrast, those of the control group were intravenously injected with 0.5 mg/kg normal saline in the same way. Heart rate (HR) and mean arterial pressure (MAP) of children were recorded at entering the operating room (T0), 2 min after induction (T1), intubation (T2), the beginning of operation (T3) and the recovery of spontaneous breathing (T4). The paediatric anaesthesia emergence delirium (PAED) scores and m-CHEOPS were performed 30 min after operation, and the PHBQ scores were performed 7 and 30 days after operation. The occurrence of NPOBCs was recorded in the two groups. The operation time, recovery time, PACU stay time and negative postoperative behavioral changes were recorded. Results The PAED score and m-CHEOPS score were lower in the test group than those of the control group, and the differences were statistically significant (P<0.05 ). PAED score was positively correlated with the m-CHEOPS score (rs=0.628, P<0.01). Separation anxiety at 7 days after operation was positively correlated with PAED score and m-CHEOPS score (rs were 0.331 and 0.401, all P<0.01). The incidence of separation anxiety and overall NPOBCs was higher in children in the control group at day 7 postoperatively compared to those of the test group (P<0.05). The incidence of NPOBCs was higher at 7 days than that at 30 days post-operatively in the control group. The recovery time and PACU stay time were significantly lower in the test group than those in the control group (P<0.05). Conclusion Esketamine is beneficial to the rapid recovery of preschool children after tonsillectomy. Figures and Tables | References | Related Articles | Metrics

Applied Research

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Relationship between pectoralis muscle index and prognosis of breast cancer patients with bone metastases based on radiomics WU Haixiao, MA Wenjuan, LI Zhijun, ZHANG Chao 2023, 51 (9):  1007-1010.  doi: 10.11958/20230241 Abstract ( 440 )   HTML ( 10 )   PDF (843KB) ( 602 )   Objective To accurately calculate the pectoralis muscle mass and clarify the influence of pectoralis muscle index (PMI) on the survival and prognosis of breast cancer patients with bone metastasis (BCBM) using radiomics. Methods Clinical data of 197 BCBM patients were collected, including age, pathological classification, distant metastasis, radiotherapy and 5-year follow-up data. The PMI was calculated based on chest CT results. The optimal cutoff value for PMI was determined by receiver operating characteristic (ROC) curve, and patients were divided into the low-PMI and the high-PMI groups. Then the clinical and pathological characteristics and prognostic differences of patients with different levels of PMI were analyzed. Results According to the optimal cutoff value for PMI, 197 BCBM patients were divided into the low PMI group (≤6.295, 104 cases) and the high PMI group (>6.295, 93 cases). Compared with the high PMI group, there were significant differences in the proportion of Ki-67≥14%, distribution of bone metastasis sites, and serum Ca2+ concentration in the low PMI group (P<0.05). The 1-, 3- and 5-year overall survival rates of patients were 67.3%, 17.3%, 5.8% and 96.8%, 84.9% and 36.6% in the low PMI group and the high PMI group, respectively, and the cumulative overall survival rate difference was statistically significant (Log-rank χ2=82.329, P<0.001). The multivariate Cox regression analysis showed that after adjusting pathological classification, site of bone metastasis, brain metastasis and Ca2+, the risk of death in the low PMI group was 3.954 times higher than that in the high PMI group. Conclusion The decrease in PMI can increase the risk of death in BCBM patients. Personalized rehabilitation training and nutritional support intervention should be encouraged for patients during the BCBM treatment process. Figures and Tables | References | Related Articles | Metrics

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Application of PET/CT radiomics combined with LncRNA-DGCR5 in precision medicine of NSCLC ZHANG Zhenhua, FU Wei, LIU Weiliang, LI Junyan, HUANG Tao, HU Hui, FAN Zhigang 2023, 51 (9):  1011-1015.  doi: 10.11958/20221865 Abstract ( 426 )   HTML ( 13 )   PDF (806KB) ( 593 )   Objective To study the application value of PET/CT radiomics combined with long noncoding RNA DiGeorge syndrome critical region gene 5 (LncRNA-DGCR5) in the precision medicine of non-small cell lung cancer (NSCLC). Methods A total of 106 NSCLC patients received PET/CT examination and chemoradiotherapy, and their efficacy was evaluated according to the Evaluation Criteria for Solid Tumor Efficacy after Treatment (RECIST). Patients with complete remission and partial remission were included in the response group (58 cases), and patients with stable or progressive disease were included in the non-response group (48 cases). Image texture parameters of PET/CT including SUVmax (maximum standard value), mean value, variance, kurtosis, skewness, contrast, entropy and energy were compared between groups. Real-time fluorescence quantitative PCR was used to detect LncRNA-DGCR5 expression in peripheral blood. Receiver operating characteristic curve (ROC) was constructed to analyze the predictive value of each index to the therapeutic efficacy of NSCLC patients. Results The SUVmax value, entropy and expression of LncRNA-DGCR5 were significantly lower in the response group than those in the non-response group (P<0.05), and the energy was significantly higher in the response group than that in the non-response group (P<0.05). There were no significant differences in the other parameters between the two groups. Patients were divided into the low LncRNA-DGCR5 expression group (51 cases) and the high LncRNA-DGCr5 expression group (55 cases) according to the mean expression of LncRNA-DGCR5 (2.01) as the critical value. Compared with the low LncRNA-DGCR5 expression group, the PET/CT image texture parameters of SUVmax and entropy were significantly increased in the high LncRNA-DGCR5 expression group, and the energy was significantly decreased (P<0.05). ROC curve construction showed that the AUCs of SUVmax value, entropy, energy and LncRNA-DGCR5 expression on predicting the treatment efficacy of patients with NSCLC were 0.897, 0.851, 0.795 and 0.949 respectively, and the AUC of combined prediction was the highest at 0.998. Conclusion PET/CT radiomics combined with LncRNA-DGCR5 is helpful to predict the treatment efficacy of patients with NSCLC, thus providing the guidance for precision medicine of NSCLC. Figures and Tables | References | Related Articles | Metrics

Review

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New research progress of exosomes in the treatment of myocardial infarction HOU Yongbo, YU Junma, ZHU Haijuan 2023, 51 (9):  1016-1019.  doi: 10.11958/20230440 Abstract ( 459 )   HTML ( 17 )   PDF (766KB) ( 609 )   Myocardial infarction is one of the most lethal diseases, and the progression of the disease often leads to the occurrence of chronic heart failure. Therefore, it is very important to explore new treatment methods for the prognosis of myocardial infarction. Exosomes are nanoscale vesicles with a diameter of 30-150 nm released from cells or tissue fluid. The vesicles carry specific DNA, RNA, proteins, lipids, metabolic waste and other substances secreted by parental cells. As a potential treatment for myocardial infarction, it has long been confirmed in basic experiments. This article reviews the latest research progress of exosomes from different cells and tissues, engineered exosomes and exosome cell membrane fusion strategies in the treatment of myocardial infarction, and provides scientific evidence for the clinical application of exosomes. References | Related Articles | Metrics

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Research progress of endocannabinoid system for prevention and treatment of osteoporosis WANG Lei, YANG Tao, GENG Licheng, SUN Tianwei 2023, 51 (9):  1020-1024.  doi: 10.11958/20230510 Abstract ( 415 )   HTML ( 12 )   PDF (770KB) ( 584 )   Osteoporosis (OP) is a kind of systemic bone disease characterized by reduced bone mass and changes of bone microstructure. The incidence rate of OP has been increasing gradually year by year, which has become a severe public health issue. The imbalance of bone metabolism mediated by osteoblasts and osteoclast is an important pathogenesis of OP. The endocannabinoid system (ECS) is widely distributed in bone tissue and participates in regulating multiple biological effects of osteoblasts and osteoclasts, suggesting that ECS may be a potential treatment target of OP. Further study on targeting ESC can provide a new theoretical basis for the clinical treatment of OP. This article reviews the specific role of ECS in regulating the pathogenesis of OP. References | Related Articles | Metrics