Abstract: Objective To observe changes of motilin(MTL) levels in gastric body, duodenum and plasma in rat model of acute incisional pain．Methods A total of 156 healthy male adult SD rats， weighing 180-220 g， were randomized into two groups: control group (group C， n=78) and incisional pain group (group P， n=78)， Rats in P group received incision on the right plantaris. Values of paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at dif⁃ ferent time points of 24 hours before operation (T0) and 1 hour (T1)， 6 hours (T2)， 24 hours(T3)， 48 hours (T4) and 72 hours (T5) after operation were measured in six rats chosen randomly from each group. Twelve rats were chosen from each group at T0-5, and sacrificed. The MTL levels in plasma, the mucosal tissues of gastric body and duodenum were detected by ELISA. Results Compared with group C, PWMT and PWTL were significantly decreased at T1-4 in group P. The MTL levels were sig⁃ nificantly decreased in plasma and gastric body (P＜0.05)．The MTL level was significantly increased at T1-4 in duodenum (P＜0.05)， and no significant changes were found at T0 and T5 in P group(P > 0.05). The plasma MTL levels were positively correlated with PWMT and PWTL (r=0.952， r=0.879， respectively， P＜0.01) in P group. The MTL levels in gastric body were positively correlated with PWMT and PWTL（r=0.970， r=0.931， respectively， P＜0.01) in P group. The MTL levels were neg⁃ atively correlated with PWMT and PWTL(r=- 0.991， r=- 0.975， respectively， P＜0.01) in duodenum in P group. Conclusion The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum．model of acute incisional pain

The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum．

Key words: motilin, stomach, duodenum, plasma, acute incisional pain