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15 March 2015, Volume 43 Issue 3 Previous Issue    Next Issue
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Cell and Molecular Biology
Experimental Study

Experimental Study
Clinical Study
Professional Research
Applied Essay
Epidemiological Investigation
Diagnostic Techniques
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Cell and Molecular Biology

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The efficiency and function detection of NK cell differentiation from human umbilical cord  hematopoietic stem cells in vitro#br# LUO Qi, YIN Jie, LI Yang, HUANG Shan, WANG Xi, HE Jinghua 2015, 43 (3):  225-228.  doi: 10.11958/j.issn.0253-9896.2015.03.001 Abstract ( 869 )   PDF (1061KB) ( 3866 )   Objective To detect the efficiency and function of NK cell differentiation from human umbilical cord hematopoietic stem cells (HSCs) in vitro. Methods CD34+ hematopoietic stem cells were isolated from human umbilical cord blood, and inoculated into SCGM medium containing 20 μg/L FMS like tyrosine kinase 3 ligand (Flt- 3L), stem cell factor (SCF), interleukin (IL) -7, IL-15 and IL-21. And CD34+ HSCs were differentiated into NK cells in directional inducing. The growth state of cells was observed. The expressions of CD56, NKG2D, NKp46, CD3, CD19 and CD34 were detected by flow cytometry in the differentiation of 7, 14, 21 and 28 d. In the differentiation of 21 d and 28 d, the differentiation cells were used as effector cells, and K562 cells as target cells. The ratios of effector cells and target cells were 8∶1, 4∶1, 2∶1 and 1∶1. The killing activity of the differentiated cells was detected by lactate dehydrogenase (LDH) cell toxicity assay and 7AAD/CFSE labeling method. Results CD34+ HSCs derived from human umbilical cord blood can proliferate in vitro under appropriate condition. There were no significant differences in the expression of CD3 and CD19 between different differentiation stages (7, 14, 21 and 28 d, P > 0.05). The expressions of CD56, NKG2D and NKp46 were significantly different (P < 0.05), and the ultimate expression amount was (72.57±1.60)%, (32.83±1.29)% and (29.53±2.40)%. The expression of CD34 decreased gradually, and the lowest was (12.13 ± 2.01)%. The maximum killing activity detected by LDH cell toxicity assay and 7AAD/CFSE labeling method reached(49.91±2.76)% and (40.87±1.12)%.The killing activity of NK cells was decreased in the order of 8∶1, 4∶1, 2∶1 and 1∶1 groups (P < 0.05). There was no significant difference in the killing activity between NK cells of 28 d and 21 d. Conclusion Human umbilical cord hematopoietic stem cells can differentiate into NK cells under appropriate conditions in vitro, and the NK cells induced from differentiation are with killing activity. Related Articles | Metrics

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Association between polymorphism of S1, S2 locus allele in ADAM33 gene and chronic obstructive pulmonary disease in Xinjiang Uygur population HAO Ee, GUAN Jian, XU Xilin, GAO Yan, ZHANG Zhonghong, WANG Shasha, WANG Shan 2015, 43 (3):  229-232.  doi: 10.11958/j.issn.0253-9896.2015.03.002 Abstract ( 824 )   PDF (334KB) ( 3817 )   Objective To investigate the association between polymorphism of S1, S2 locus allele in ADAM 33 gene and chronic obstructive pulmonary disease (COPD) and lung function in Xinjiang Uygur population. Methods Blood samples from 217 COPD patients and 218 healthy controls were collected. Samples of DNA was extracted, and S1, S2 single nucleotide polymorphism (ADAM 33) was detected by ABI SNaPshot SNP genotyping. Results There were no significant differences in the frequencies of S1 locus CC, CT, TT genotypes and C, T alleles between patient group and control group (P > 0.05). There were no significant differences in the frequencies of S1 locus CC, CG, GG genotypes and C, G alleles between patient group and control group (P > 0.05). In patient group, there were no significant differences in S1, S2 locus genotype and clinical indicators of lung function display, and in the FEV1% predicted and FEV1/FVC (P > 0.05). Haplotype analysis showed that there were no significant differences in three kinds of haplotypes between patient group and control group (P > 0.05). Conclusion There is no significant difference in the polymorphism of S1, S2 locus allele in ADAM 33 gene and the susceptibility to COPD in Xinjiang Uygur population. Related Articles | Metrics

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Construction of novel 3-D composite bionic network and evaluation of its histocompatibility CUI Man， ZHANG Xin， MA Litao， XiongYanjie， CHE Pengcheng， YAO Fanglian， SUN Hong 2015, 43 (3):  233-236.  doi: 10.11958/j.issn.0253-9896.2015.03.003 Abstract ( 638 )   PDF (1368KB) ( 3761 )   Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Methods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ratio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabilities and the degradation of the material were observed 2, 4, 8 and 12 weeks after surgery. Results The structure of novel 3-D composite bionic network was three-dimensional and porous. The cells attached on scaffolds attached and grew well with polygonal or fusiform form. It was found that inflammatory reactions were alleviated gradually in the early stage . There was an increasing angiogenesis at late stage. Materials degraded and absorbed more slowly. Conclusion The present study suggests that the novel 3-D composite bionic network has good histocompatibility with easy vascularization, and will be a candidate scaffold for bone tissue engineering. Related Articles | Metrics

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The effects of scutellarin on apoptosis and the expression of caspase-8 in human tongue squamous carcinoma Tca8113 cells ZHANG Ying, LI Junmei, QIN Bowen, GE Zhihua 2015, 43 (3):  237-240.  doi: 10.11958/j.issn.0253-9896.2015.03.004 Abstract ( 746 )   PDF (620KB) ( 3908 )   Objective To investigate the possible mechanism of scutellarin on the apoptosis of human tongue squamous carcinoma Tca8113 cells. Methods Human tongue squamous carcinoma Tca8113 cells were divided into control group and scutellarin groups (80, 120 and 160 mg/L). Tunel method was used to detect the apoptosis. Immunohistochemistry and Western blot assay was used to detect the caspase-8 protein expression of cells. Results （1）The apoptotic rates of Tca8113 cells were significantly high in scutellarin groups (80, 120 and 160 mg/L) than those in control group [(17.63 ± 0.25)%, (36.23±0.36)%, (51.84±0.58)% vs (4.45±0.27)%, P < 0.05].（2） Compared with control group, the expressions of caspase-8 protein were significantly increased in different concentrations (80, 120 and 160 mg/L) of scutellarin groups (0.283±0.040 vs 0.474±0.031, 0.592±0.077, 0.781±0.020， P < 0.05). Conclusion Scutellarin could obviously induce the apoptosis of human tongue squamous carcinoma Tca8113 cells, which may be related to the caspase-8 protein expression. Related Articles | Metrics

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The effect of zinc finger protein 185 on the proliferation of human glioma cells LU Bin, ZHENG Quanhui 2015, 43 (3):  241-244.  doi: 10.11958/j.issn.0253-9896.2015.03.005 Abstract ( 714 )   PDF (532KB) ( 3837 )   Objective To explore the role of zinc finger protein (ZNF)185 in the proliferation of human glioma cells. Methods Human glioma tissues and tumor adjacent tissues were obtained from glioma patients diagnosed pathologically in Tangshan Gongren Hospital from January 2011 to December 2013. Total protein was extracted from different tissues. The ZNF185 expression was detected by Western-blot assay. Total RNA was extracted from tumor adjacent tissues. ZNF185 coding sequence was obtained by RT-PCR and inserted into pEGFPC2 plasmid to construct the ZNF185 expression vector. Lipofactamine2000 was used to transfect the ZNF185 expression vector to human glioma cell SF767. pEGFPC2 blank vector transfected SF767 cells were used as control. Changes of cell cycle were analyzed by flow cytometry, and cell proliferation was analyzed by MTT assay. Results The expression of ZNF185 decreased significantly in human glioma tissues compared to that of tumor adjacent tissues（P < 0.01). The over expression of ZNF185 in SF767 resulted in the increased proportion of cell cycle G0-G1phase, but decreased proportion of S phase（P < 0.05). Furthermore, the cell proliferation was significantly inhibited in the ZNF185 over-expressed SF767 cells compared with that of blank vector transfected cells（P < 0.05). Conclusion ZNF185 plays an inhibitory role in cell proliferation of human brain glioma cells. Related Articles | Metrics

Experimental Study

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Effects of ginsenoside Rg1 on PARP-1 and TNFR1 expression in rat model of focal cerebral ischemia YU Yang1, LIU Xuezheng1△, BAO Cuifen2, LI Xiaoming3, LIU Xia3 2015, 43 (3):  245-248.  doi: 10.11958/j.issn.0253-9896.2015.03.006 Abstract ( 817 )   PDF (1893KB) ( 3918 )    Objective To explore effects of ginsenosides Rg1 on the expression of poly(ADP- ribose) polymerase1 (PARP- 1) and tumor necrosis factor receptor (TNFR) 1 in cortex cells after focal cerebral ischemia in rats. Methods Ninety healthy rats were randomly divided into sham-operative group, focal cerebral ischemia group, ginsenoside Rg1groups (low, medium and high concentrations) and drug control group. Rats were intraperitoneally injected saline 45 mg/kg, saline 45 mg/kg+ginsenosides Rg1 10, 20 and 40 mg/kg, nimodipine 1 mg/kg 5 d before surgery, respectively. Focal cerebral ischemia model was made by middle cerebral artery occluding in rats. The neurological deficit score and TTC staining were used to verify the success of the rat model. The expressions of PARP-1 and TNFR1 were evaluated by immunohistochemical method and Western blot technique. Results There were obvious symptoms of neurological deficit and large pale infarct area in focal cerebral ischemia group compared with those of sham-operative group. There were higher percentages of neurological deficit score and infarct area in ginsenosides Rg1 groups and positive control group than those of sham-operative group, but which were lower than those of ischemia group (P＜0.05). There were no significant differences between ginsenosides Rg1 groups and positive control group. The positive cells of PARP- 1 and TNFR1 were higher in ginsenosides Rg1 low- dose group than those of sham-operative group and positive control group, while ones of medium and high-dose Rg1 group were higher than those of sham-operative group, and were lower than those of ischemia group (P＜0.05). Compared with sham-operative group, PARP-1 and TNFR1 expression strips were significantly enhanced in ischemia group. Expression strips were higher in ginsenosides Rg1 low-dose group than those of shamoperative group. Expression strips were higher in ginsenosides Rg1 medium-dose group than those of sham-operative group, but which were lower than those of ischemia group, and ones of high-dose group were lower than ischemia group (P＜0.05). Conclusion Ginsenoside Rg1 shows protective effects on focal ischemia injury, which may be related with down-regulation of the expression of PARP-1 and TNFR1. Related Articles | Metrics

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Expression and significance of Six1 in cervical cancer WANG Tong1, PIAO Longzhen1, CUI Xuelian2, YANG Yang2, NAN Yunze1△ 2015, 43 (3):  249-251.  doi: 10.11958/j.issn.0253-9896.2015.03.007 Abstract ( 790 )   PDF (614KB) ( 4798 )   Objective To investigate the clinical significance of protein expression of Six1 in cervical cancer. Methods The immunohistochemical (IHC) staining was applied to detect the expression of Six1 protein in normal cervical tissues (n=32), cervical intraepithelial neoplasia (CIN) tissues (n=49) and cervical cancer tissues (n=123). The localization of Six1 protein was detected in vitro cultured HeLa cells using immunofluorescence (IF) staining. Results The positive rate of Six1was significantly higher in cervical cancer (72.3%) than that of CIN tissues (28.6%) and normal cervical tissues (15.6%, 2χ = 13.118 and 10.058 respectively, P < 0.01). There were significance differences in expression levels of Six1 protein between different tumor sizes and metastasis of cervical cancer (P < 0.01). The Six1 protein showed positive signals in cytoplasm and nucleoli in HeLa cells. Conclusion Six1 expression is associated with cervical cancer, which may be a potential biomarker for invasion and metastasis of cervical cancer. Related Articles | Metrics

Experimental Study

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The protective effect of glutamine pretreatment on intestinal ischemia-reperfusion injury and eNOS/NO levels in rats#br# #br# NIU Qiong， WANG Aili， WANG Wei， HU Yingbin， LIU Chengxia 2015, 43 (3):  252-255.  doi: 10.11958/j.issn.0253-9896.2015.03.008 Abstract ( 799 )   PDF (469KB) ( 3986 )   Objective To investigate the protective effect of glutamine(Gln) pretreatment on intestinal ischemia-reperfusion (I/R) injury and endothelial nitric oxide synthase (eNOS) / nitric oxide (NO) signaling pathway in rat model. Methods Thirty male Wistar rats were randomly divided into three groups(n=10 for each group): sham group, I/R group and Gln group. Animals were pretreated with 1 g/ （kg·d）Gln by orogastric route for 7 days in Gln group, and normal saline was given to the other two groups in the same dose. Intestinal I/R was induced by 30 min occlusion of the superior mesenteric artery followed by 24 h of reperfusion. After the operation, the intestinal histopathological changes, the plasma endotoxin level, serum D-lactic acid, eNOS, inducible NOS(iNOS)activity and NO levels were detected by ultraviolet spectrophotometer. The mRNA expressions of myocardial eNOS and iNOS were detected by real-time fluorescence quantitative PCR (RT-PCR). Results After reperfusion, in IR group, extensive epithelial sloughing and mucosal ulceration of villous tips were observed, whereas these findings did not occur in Gln group and sham group. Compared with IR group, the serum NO, eNOS levels and eNOS mRNA expression of intestinal tissue were elevated in Gln group (P < 0.01), but the plasma endotoxin level, serum D-lactic acid, serum iNOS and intestinal iNOS mRNA expression decreased in IR group(P < 0.05). Conclusion Glutamine pretreatment has protective effects on intestinal ischemia-reperfusion injury in vivo. The mechanism may be related to the inhibition of iNOS expression and the increased expression of eNOS, thereby increasing NO activity. Related Articles | Metrics

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The effects of vardenafil on high altitude pulmonary hypertension in rats ZHOU Xiaoling, PAN Lei, MA Tingting, GUO Rui, WANG Yong 2015, 43 (3):  256-258.  doi: 10.11958/j.issn.0253-9896.2015.03.009 Abstract ( 890 )   PDF (918KB) ( 3943 )   Objective To investigate the effect of vardenafil on high altitude pulmonary hypertension in rats, and the possible mechanism thereof. Methods Thirty rats were randomly divided into three groups: control group with normal-pressure and normal-oxygen (group C), pulmonary hypertension group with low-pressure and low-oxygen (group P), and the group treated by vardenafil in low-pressure and low-oxygen condition (group V). The rats of group P and group V were exposed to low-pressure and low-oxygen condition in an auto-modulating hypobaric and hypoxic cabin to simulate 5 000 m high altitude environment (air pressure 50 kPa, oxygen concentration 10%) for 8 hours daily. Vardenafil (1 mg/kg) was given by gastrogavage to rats in group V once daily for 4 weeks, while the isodose distilled water was given by gastrogavage to rats in group C and group P. The mean pulmonary arterial pressure and right ventricular mass index were measured respectively after 4-week treatment. Morphologic changes of peripheral pulmonary artery were detected by light microscope. The serum levels of nitric oxide (NO) and endothelin-1 (ET-1) were detected as well. Results The pulmonary arterial pressure and right ventricular mass index were significantly higher in group P than those of group C and group V (P < 0.05). The ratio of vascular medial wall thickness to external diameter (WT%) and the ratio of pulmonary artery wall area to tube area (WA%) were significantly increased in group P than those of group C and group V (P < 0.05). Furthermore, the serum level of NO was significantly lower in group P than that of group C and group V, but the serum level of ET-1 was significantly increased compared with that of group C and group V (P < 0.05). Conclusion Vardenafil can effectively reduce the pulmonary arterial pressure, and attenuate pulmonary vessels and right ventricle remodeling induced by high altitude pulmonary hypertension. Related Articles | Metrics

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The influence of carnosine in expression levels of bcl-2 and bax after focal cerebral ischemia in rats#br# ZHU Jie， MA Qian， WANG Xin， LIU Cuimei， WANG Aihong 2015, 43 (3):  259-262.  doi: 10.11958/j.issn.0253-9896.2015.03.010 Abstract ( 744 )   PDF (1277KB) ( 3841 )   Objective To explore the effect of carnosine in the expression of B cell lymphomal/leukemia-2 (bcl-2) and bcl-2-associated X protein (bax) after focal cerebral ischemia in rats. Methods Thirty male SD rats (SPF scale) were randomly divided into 3 groups: sham-operated group, model group and carnosine treated group (n=10 for each group). The middle cerebral artery occlusion model (MCAO) was induced in model group and carnosine treated group. Rats were received carnosine [1 000 mg/(kg·d), orally] in carnosine treated group, and the other rats were received the same volume of normal saline (NS) in shame-operated group and model group. The neurological deficit score was used to evaluate the neurological function at 24 h and 72 h after MCAO. Morphological changes were observed by HE staining. TCC staining was used to label infarct volume, and immunohistochemistry was used to detect the expression of bcl- 2 and bax. Results Compared with model group, the score of neurological function and infarct volume were significantly declined in carnosine treated group at 72 h after injury (P<0.05 or P<0.01). The changes of ischemic impairment were lighter in carnosine treated group than that of model group. Compared with sham-operated group, the expression levels of bcl- 2 and the ratio of bcl-2/bax were decreased while the expression of bax was increased in model group (P<0.05). Compared with model group, carnosine could significantly increase the expression of bcl-2 and the ratio of bcl-2/bax, and reduce the expression of bax (P<0.01 or P<0.05). Conclusion Carnosine can enhance bcl-2 expression, decrease bax expression and increase the ratio of bcl-2/bax, which is likely to be one of the mechanisms of neuroprotection. Related Articles | Metrics

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Changes of motilin concentrations in plasma and upper gastrointestinal tract in rat model of acute incisional pain ZHAO Jun1， XU Lei2△， ZHANG Yu3， FENG Quansheng2 2015, 43 (3):  263-266.  doi: 10.11958/j.issn.0253-9896.2015.03.011 Abstract ( 647 )   PDF (306KB) ( 3889 )   Objective To observe changes of motilin(MTL) levels in gastric body, duodenum and plasma in rat model of acute incisional pain．Methods A total of 156 healthy male adult SD rats， weighing 180-220 g， were randomized into two groups: control group (group C， n=78) and incisional pain group (group P， n=78)， Rats in P group received incision on the right plantaris. Values of paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at dif⁃ ferent time points of 24 hours before operation (T0) and 1 hour (T1)， 6 hours (T2)， 24 hours(T3)， 48 hours (T4) and 72 hours (T5) after operation were measured in six rats chosen randomly from each group. Twelve rats were chosen from each group at T0-5, and sacrificed. The MTL levels in plasma, the mucosal tissues of gastric body and duodenum were detected by ELISA. Results Compared with group C, PWMT and PWTL were significantly decreased at T1-4 in group P. The MTL levels were sig⁃ nificantly decreased in plasma and gastric body (P＜0.05)．The MTL level was significantly increased at T1-4 in duodenum (P＜0.05)， and no significant changes were found at T0 and T5 in P group(P > 0.05). The plasma MTL levels were positively correlated with PWMT and PWTL (r=0.952， r=0.879， respectively， P＜0.01) in P group. The MTL levels in gastric body were positively correlated with PWMT and PWTL（r=0.970， r=0.931， respectively， P＜0.01) in P group. The MTL levels were neg⁃ atively correlated with PWMT and PWTL(r=- 0.991， r=- 0.975， respectively， P＜0.01) in duodenum in P group. Conclusion The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum．model of acute incisional pain The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum． Related Articles | Metrics

Clinical Study

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The expression of miR-451 and its clinical significance in gastric cancer CHEN Tao， XIE Liming， TANG Hailin 2015, 43 (3):  267-269.  doi: 10.11958/j.issn.0253-9896.2015.03.012 Abstract ( 889 )   PDF (1024KB) ( 4139 )   Objective To investigate the expression of miR-451 and its clinical significance in gastric cancer. Methods The miR-451 expression was detected in 92 samples of gastric cancer and 47 sapmles of normal stomach mucosa. The correlations of miR-451 expression with clinicopathologic characteristics, overall survival and disease-free survival were an⁃ alyzed in patients with gastric cancer. Results The overexpression rate of miR-451 was 38.04% in gastric cancer, which was significantly lower than 76.60% in adjacent normal tissues (χ2=18.503， P＜0.001). The overexpression rate of miR-451 was significantly lower in Ⅲ-Ⅳ stages of gastric cancer than that in Ⅰ-Ⅱ stages (27.3% vs 54.1%, P=0.009). The expres⁃ sion of miR-451 was also lower in patients with lymph node metastasis than that in patients without lymph node metastasis (27.1% vs 57.6%, P=0.004). However, there were no significant differences in miR-451 expressions between different age, gender, histological grade and T stage groups (P > 0.05). The overall survival rate was significantly higher in patients with high expression of miR-451 than that in patients with low expression of miR-451(69.2% vs 39.1%, P=0.003). Conclusion miR- 451 is associated with the malignant transformation, progression, and prognosis of gastric cancer, and which can be used as a potential target of therapy and a biomarker of diagnosis and prognosis of gastric cancer in the future. Related Articles | Metrics

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The expression of COX-2 and Survivin in ameloblastoma and its clinical significance LI Le1, ZHANG Xingle1, WANG Peng1, LIU Yu2 2015, 43 (3):  270-273.  doi: 10.11958/j.issn.0253-9896.2015.03.013 Abstract ( 696 )   PDF (606KB) ( 4035 )   Objective To investigate the expression of cyclooxygenase-2（COX-2） and Survivin in ameloblastoma (AB) tissues. Methods A total of 60 AB samples (primary AB 40 cases, recurrent AB 20 cases) and 60 normal oral mucosas were collected from the Affiliated Hospital of Chengde Medical College. The AB samples included 12 acanthomatous types, 18 follicular patterns, 18 plexiform patterns, 6 basal cell ameloblastomas and 6 desmoplastic ameloblastomas. The expression levels of COX-2 and Survivin were detected by immunohistochemistry and Western blot methods in AB and normal oral mucosa. The expressions of COX-2 and Survivin were compared between different types and tissues of AB. Results The positive rate of COX-2 was significantly higher in AB (82.7%) than that in normal oral mucosa (10.0%). The positive rate of Survivin was significantly higher in AB (83.3%) than that in normal oral mucosa tissues (16.7%). The expression of COX-2 (0.781±0.142) was higher in AB than that of normal oral mucosa tissues (0.179±0.056). The expression of Survivin (0.447± 0.139) was significantly higher in AB than those in normal oral mucosa tissues (0.072±0.017). There was a positive correlation between the expression of COX-2 and Survivin (r=0.778， P＜0.05). There were no significant differences in the positive expressions of COX-2 and Survivin between different types of AB (P>0.05). Conclusion COX-2 and Survivin were overexpressed in AB, which may be involved in the occurrence and development of AB. Related Articles | Metrics

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Researches on inflammatory factor and blood coagulation in patients with laparoscopic gastric cancer radical surgery LU Li, XIE Min, XIONG Zhiguo 2015, 43 (3):  274-277.  doi: 10.11958/j.issn.0253-9896.2015.03.014 Abstract ( 875 )   PDF (280KB) ( 3924 )   Objective To investigate the change of inflammatory factor and blood coagulation in patients with laparo⁃ scopic gastric cancer radical surgery. Methods Data of seventy-seven stomach cancer patients hospitalized in our hospital and received the surgical treatment from June 2012 to June 2014 were retrospectively analyzed. Thirty-eight of these pa⁃ tients underwent laparoscopic treatment (laparoscopic group) and 39 cases received laparotomy treatment (laparotomy group). The values of interleukin-6 (IL-6), IL-8 and tumor necrosis factor alpha (TNF-α) were analyzed at the time points of preop⁃ eration and 6 h, 24 h after the surgery in two groups. The levels of activated partial thromboplastin time (APTT), prothrombin time (PT), international standardization ration (INR), fibrinogen (FIB) and D dimer (D-D) were also analyzed at the same time points in two groups. The postoperative thrombosis and tumor recurrence rate were also analyzed in two groups of pa⁃ tients. Results There were no significant differences in the gender, age, the size of tumor, differentiation degree and TNM staging between two groups of patients. The serum levels of IL-6, IL-8 and TNF-α were significantly increased after surgery in two groups (P ＜ 0.01), and which were significantly higher in laparotomy group than those of laparoscopic group (P＜ 0.01). The levels of APTT, PT and INR were significantly lower after surgery in two groups of patients, and which were signifi⁃ cantly lower in laparoscopic group than those of laparotomy group (P＜0.05). The levels of FIB and D-D were significantly increased after operation in two groups of patients (P＜0.05), which were significantly higher in laparoscopic group than those of laparotomy group (P＜0.05). The postoperative recurrence of cancer was less in laparoscopic group than that of lapa⁃ rotomy group. But, the postoperative thrombosis was more in laparoscopic group than that of laparotomy group. Conclusion There was less release of inflammatory mediators and less effect on the body’ s immune function in patients with laparoscopic gastric cancer radical surgery. But, the surgery has a large influence in blood coagulation, we should pay attention to it in the clinical treatment. Related Articles | Metrics

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The influence of CsA in CD40/CD40 ligand expression of T lymphocytes in patients with coronary artery disease LIU Huan1， PENG Changsheng1， HU Yuecheng1， LI Ximing2， CONG Hongliang2△ 2015, 43 (3):  278-281.  doi: 10.11958/j.issn.0253-9896.2015.03.015 Abstract ( 694 )   PDF (390KB) ( 3785 )   Objective To investigate the effect of CD40/CD40 ligand on the genesis and development of coronary artery disease (CAD)， and the inhibitory effect of cyclosporine A (CsA) on CD40/CD40 ligand. Methods A total of 71 patients were divided into four groups: acute myocardial infarction group (AMI, n=19), unstable angina pectoris group (UAP, n=18), stable angina pectoris group (SAP, n=17) and normal control group (N, n=17). Flow cytometry was used to detect the expression of CD40 and CD40L in peripheral blood mononuclear cells (PBMCs) of four groups. The group in which CD40 and CD40L were produced at the highest level was chosen, and a series concentrations of CsA（H1:0 mg/L, H2:0.01 mg/ L, H3: 0.1 mg/L, H4:1 mg/L） were used to treat the cells. Then the expressions of CD40 and CD40L were measured by flow cytometry. Results Compared with N group， the expression of CD40 was significant higher in other groups (P < 0.05). The expression of CD40 was significant higher in UAP group and AMI group than that of SAP group. There was no significant difference in the expression of CD40 between UAP group and AMI group (P > 0.05). The expression of CD40L was elevated and fol⁃ lowed by different severity of CAD. There was significant difference in the expression of CD40L between groups (P < 0.05) . AMI group showed the highest expression of CD40 and CD40L. After being treated with CsA, the expression of CD40 was higher in H1 group than that of H3 group and H4 group (P < 0.05). The expression of CD40L was significantly higher in H1 group than that of other three groups (P < 0.05). Conclusion CD40 and CD40L may be involved in the development of CAD. Moreover, it might be restrained by CsA via regulation of CD40/CD40L. Related Articles | Metrics

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The expression and significance of MDSCs in peripheral blood in patients with peripheral T cell lymphoma LI Yanli, Bu Qing 2015, 43 (3):  282-284.  doi: 10.11958/j.issn.0253-9896.2015.03.016 Abstract ( 945 )   PDF (292KB) ( 3896 )   Objective To study the relationship between the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood and prognosis in patients with peripheral T-cell lymphoma (PTCL). Methods Twenty-three patients with PTCL were selected in this study and 14 healthy persons were used as the normal control. The proportion of MDSCs/ mononu⁃ clear in peripheral blood was detected by flow cytometry. The correlation between the number of MDSCs and the clinical character and prognosis in patients with PTCL was analyzed. Results The proportion of MDSCs in peripheral blood was sig⁃ nificantly higher in patients with PTCL than that of healthy subjects [(4.59±1.41)% vs (1.36±0.59)%, P < 0.01]. The number of MDSCs in peripheral blood was significantly decreased after chemotherapy compared with that before treatment [(1.29 ± 2.95)%, P < 0.05)]. There were significant differences in MDSCs expressions in patients between different extranodal involve⁃ ment, bone marrow involvement, clinical stage and IPI score (P < 0.05). Conclusion The higher expression of MDSCs may be related to the progression in patients with PTCL. Related Articles | Metrics

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Effects of loading dose of atorvastatin on inflammatory cytokines in acute myocardial infarction patients undergoing emergency percutaneous coronary intervention SHAN Rui1, WU Shangqin2, CHENG Aijuan2△ 2015, 43 (3):  285-287.  doi: 10.11958/j.issn.0253-9896.2015.03.017 Abstract ( 839 )   PDF (334KB) ( 4035 )   Objective To investigate the effects of high loading dose of atorvastatin on lipoprotein-associated phospho⁃ lipase A2 (Lp-PLA2) and inflammatory cytokines in patients with acute myocardial infarction (AMI), who underwent emergen⁃ cy percutaneous coronary intervention (PCI). Methods A total of 65 cases with AMI who underwent emergency PCI be⁃ tween October 2011 and August 2013 were randomly divided into two groups: control group (n=32, atorvastatin 20 mg/24 h) and high dose atorvastatin group (n=33, atorvastatin 40 mg/24 h). Two groups of patients were given the same basic treat⁃ ment. Blood samples were obtained before treatment and 72 h after PCI in two groups. Levels of Lp-PLA2, interleukin-6 (IL- 6), tumor necrosis factor- α (TNF-α), alanine transaminase (ALT) and aspartate transaminase (AST) were detected in two groups. The adverse drug reactions were observed. Results There were no significant differences in Lp-PLA2, IL-6, TNF- α, ALT and AST levels between two groups (P＞0.05). After PCI, the levels of Lp-PLA2, IL-6 and TNF-α were significantly increased compared with those of baseline in two groups, and they were more notable in control group than those of high dose atorvastatin group (P＜0.05). The levels of ALT and AST were also significantly higher after operation compared with those of basic levels (P＜0.05), while there were no significant differences in ALT and AST after PCI between two groups (P＞0.05). Conclusion The high loading dose of atorvastatin in AMI patients underwent emergency PCI can de⁃ crease the inflammation and stabilize the plaques in acute stage, and which is safe. Related Articles | Metrics

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The predictive value of deceleration capacity of heart rate for sudden cardiac death after acute myocardial infarction LI Yang, QIN Qin△, KOU Lu, CHEN Gang, LU Yaru 2015, 43 (3):  288-291.  doi: 10.11958/j.issn.0253-9896.2015.03.018 Abstract ( 592 )   PDF (324KB) ( 4051 )   Objective To investigate the predictive value of the non-invasive indicator deceleration capacity of heart rate (DC) in the sudden cardiac death (SCD) after acute myocardial infarction. Methods A total of 417 myocardial infarc⁃ tion patients with ST-segment elevation in sinus rhythm were enrolled in this study. DC was assessed from data of 24-hour ECG Holter. Regular follow-ups were carried out within 12 months. The SCD events were recorded and compared with patients without SCD. Results During 12 months of follow-up, 20 patients were died due to SCD (4.8%). Compared with sur⁃ vival group, patients showed significantly lower left ventricular ejection fraction (LVEF, 0.393 ± 0.065 vs 0.528 ± 0.042, P＜0.05) and DC [(2.85±1.66) ms vs (5.49±1.71) ms， P＜0.05]in SCD group. Multivariate Cox proportional hazard regression analysis showed that lower LVEF(＜0.35)［RR: 2.167(1.384- 4.661), P=0.013］and DC (DC＜4.5 ms)［RR: 3.706(2.709- 5.374)， P=0.020］ were risk factors for the occurrence of SCD. The prediction sensitivity by the decreased LVEF and DC was 52.1% and 76.4% respectively, and the specificity was 84.5% and 86.1% respectively. Conclusion The decreased value of DC after acute myocardial infarction can predict the SCD events. Related Articles | Metrics

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Comparison study of risk factors between premenopausal and postmenopausal female patients with coronary heart disease SU Bin1, QIN Qin2△, LI Yang2 2015, 43 (3):  292-295.  doi: 10.11958/j.issn.0253-9896.2015.03.019 Abstract ( 824 )   PDF (369KB) ( 3881 )   Objective To explore changes of risk factors between premenopausal and postmenopausal female patients with coronary heart disease (CHD). Methods A total of 283 female patients were divided into CHD group (n=154) and con⁃ trol group (n=129) according to whether they suffered from CHD or not. Patients were further stratified into two groups accord⁃ ing to menopausal situation: premenopausal group (n=78, including premenopausal CHD group of 34 cases and premenopaus⁃ al control group of 44 cases) and postmenopausal group (n=205, including postmenopausal CHD group of 120 cases and post⁃ menopausal control group of 85 cases). Clinical data of age, body mass index (BMI), medical history and menses situation were recorded. The levels of cholesterol (CHO), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipo⁃ protein cholesterol (LDL-C), very low density lipoprotein cholesterol (vLDL-C), apolipoprotein AI (ApoAI), apolipoprotein B (apoB), lipoprotein a (Lpa), homocysteine (HCY) and high-sensitive C-reactive protein (hsCRP) were also detected. Results There were significantly higher levels of age, CHO, TG, LDL-C, vLDL-C, Lpa, ApoB, hsCRP, and the ratio of hypertension history, diabetic history, coronary heart disease family history in CHD group than those of control group. The level of HDLc was significantly lower in CHD group than that of control group. The ratio of hypertension history, diabetic history and levels of CHO, TG, LDL-C, vLDL-C, Lpa, ApoB, hsCRP were significantly higher in both premenopausal and postmenopausal CHD groups than those of their control groups. The level of HDL-C was significantly lower in postmenopausal CHD group than that of postmenopausal control group (P＜0.05). Hypertension history, diabetic history and LDL-C were the independent risk fac⁃ tors of premenopausal CHD. Hypertension history, diabetic history and CHO were the independent risk factors of postmeno⁃ pausal CHD, and HDL-C was the protecting factor of CHD. The ratio of hypertension history, the level of CHO and LDL-C were significantly higher in postmenopausal CHD group than those of premonopausal CHD group. Conclusion The abnormal blood pressure level and dyslipidemia are more prevalent in postmenopausal female patients with CHD than premeno⁃ pausal female patients. We should control these risk factors to improve the prognosis. Related Articles | Metrics

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Study of correlation between serum potassium concentration and the severity of coronary artery disease#br# NIU Xuening, LI Guangping△ 2015, 43 (3):  296-299.  doi: 10.11958/j.issn.0253-9896.2015.03.020 Abstract ( 528 )   PDF (403KB) ( 4011 )   Objective To determine the association between serum potassium level and the severity of coronary artery atherosclerosis. Methods A total of 246 patients underwent coronary artery angiography were included into this study, and were divided into four groups according to the involved main coronary artery: control group (0 diseased vessel, n=81), one diseased vessel group (n=43), double diseased vessel group (n=46) and three diseased vessel group (n=76). Patients were also divided into low potassium group (K +<4.0 mmol/L, n=99) and high potassium group (K +≥4.0 mmol/L, n=147) according to the levels of serum potassium. The severity of coronary stenosis was quantitated by Gensini score system. The clinical data were compared between groups. The relationship between Gensini score system and clinical information was analyzed. The multiple regression method was used to analyse the risk factors of coronary heart disease (CHD). Results (1) There were higher percentages of elders, male, smoking, diabetes mellitus and hypertension patients in three diseased vessel group than those of control group. The serum potassium level was significantly lower in three diseased vessel group (3.97 mmol/L±0.37 mmol/L) than that of control group (4.11 mmol/L±0.33 mmol/L) and one diseased vessel group (4.13 mmol/L±0.41 mmol/L). There were higher percentages of elders, male and hypertension patients in double diseased vessel group than those of control group. The percentages of male and hypertension patients were higher in one diseased vessel group than those of control group. The Gensini scores were three diseased vessel group > double diseased vessel group > one diseased vessel group > control group (P < 0.05). (2) There was a significantly higher Gensini score in low potassium group [36(8, 94)] than that of high potassium group [16（0， 56)]. (3) There was significant negative correlation between serum potassium level and Gensini score (r=-0.206, P=0.001). (4) It was found that age, male, the low level of serum potassium, diabetes mellitus and hypertension were independent risk factors of CHD. Conclusion The serum potassium level is negatively correlated with the severity of CHD. The low level of serum potassium is an independent risk factor of CHD. Related Articles | Metrics

Applied Essay

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How to improve the pregnancy outcome by optimizing embryo cryopreservation protocol ZHANG Yue1,2, ZHANG Yunshan1△, XUE Fengxia2 2015, 43 (3):  300-303.  doi: 10.11958/j.issn.0253-9896.2015.03.021 Abstract ( 638 )   PDF (340KB) ( 4756 )   Objective To investigate how to optimize the protocol of embryo cryopreservation to improve the success of frozen-thawed embryo transfer (FET), reduce multiple pregnancy rate and increase the cumulative pregnancy rate from one oocyte retrieval process. Methods The clinical data of 1 166 FET cycles were retrospectively analyzed and separated into different groups： 445 for vitrification and 721 for slow-freezing. The vitrification group was divided into single embryo (28 cy⁃ cles), double embryos (71 cycles) and triple embryos (346 cycles). 0-1 optimal embryo was called O0-1 group (235 cycles), 2 optimal embryos were called O2 group (80 cycles), 3 optimal embryos were called O3 group (130 cycles). The difference preg⁃ nancy outcomes (implantation rate, clinical pregnancy, abortion rate and live- birth rate) were compared between groups. Results (1) There were significantly higher embryo survival rate（98.3% vs 73.1%), embryo recovery rate without damaging （83.3% vs 62.1%), implantation rate（36.8% vs 29.9%), clinical pregnancy（57.1% vs 44.0%) and live-birth rate（47.9% vs 34.5%) in vitrification group than those of slow freezing group（P＜0.05). (2) There were significantly higher clinical pregnan⁃ cy rate（62.7% vs 39.4% vs 32.1%), multiple pregnancy rate（37.6% vs 10.7% vs 0%） and live-birth rate（52.6% vs 31.0% vs 21.4%）in triple embryos group than those of the single and double embryos groups (P＜0.05). (3) There were significantly higher implantation rate（33.0% vs 27.1%), clinical pregnancy（65.2% vs 48.1%), multiple pregnancy rate（38.0% vs 20.4%) and live-birth rate（56.2% vs 39.2%) in O2-3 group than those of O0-1 group（P＜0.05). (4) There were significantly higher im⁃ plantation rate（36.8% vs 26.3%) and multiple pregnancy rate (46.7% vs 21.3%) in O3 group than those of O2 group (P＜0.05). There were no significant differences in clinical pregnancy（69.2% vs 58.8%), abortion rate（15.6% vs 10.6%), ectopic pregnancy rate（0 vs 0), live-birth rate（58.5% vs 52.5%) and preterm birth rate（26.3% vs 14.3%) between two groups (P＞0.05). Conclusion Vitrification technology can improve the clinical pregnancy and live-birth rate, and decrease multiple preg⁃ nancy rate. Two optimal embryos in one tube are supposed to be the preferred method for embryo cryopreservation. Related Articles | Metrics

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The predictive value of CHADS2 and CHA2DS2-VASc score in left atrial or left atrial appendage thrombus in patients with non-valvular atrial fibrillation MA Junwei， MA Xianghong△， CUI Lijun， LI Zhao 2015, 43 (3):  304-307.  doi: 10.11958/j.issn.0253-9896.2015.03.022 Abstract ( 854 )   PDF (382KB) ( 4251 )   Objective To investigate the predictive value of CHADS2 and CHA2DS2- VASc score in left atrial (LA) or left atrial appendage (LAA) thrombus in patients with non-valvular atrial fibrillation (AF). Methods A total of 164 consecu⁃ tive non-valvular AF patients confirmed by transesophageal echocardiography (TEE) were included in this study, and were divided into two groups, LA/LAA thrombus group and non LA/LAA thrombus group. The previous history was recorded in two groups of patients. Their CHADS2 and CHA2DS2-VASC scores were calculated based on the general data of two groups. The laboratory examinations and TEE indexes were compared between two gropes. Results Of all patients, the rate of LA/ LAA thrombus was 19.5%. The CHADS2 and CHA2DS2-VASC scores were significantly higher in LA/LAA thrombus group than those of non LA/LAA thrombus group (2.1±1.3 vs 1.0±0.9, 3.4±1.8 vs 1.9±1.4， P＜0.01). There were 13(11.9%) and 5 (8.8%) patients showed LA/LAA thrombus under low-moderate risk in the two score systems, respectively. Multivariate logis⁃ tic regression analysis showed that CHADS2 score ≥2 points(OR=3.735, 95%CI:1.508-9.251, P=0.004) and CHA2DS2-VASc score ≥2 points (OR=5.104, 95%CI:1.586- 16.425, P=0.006) were independent risk factors of LA/LAA thrombus. ROC curve showed that AUC of CHADS2 and CHA2DS2-VASc scores to predict LA/LAA thrombus were 0.731 (95%CI:0.630- 0.832, P＜0.001) and 0.742 (95%CI:0.640- 0.843, P＜0.001), respectively. Conclusion CHADS2 and CHA2DS2- VASC score ≥2 points are independent risk factors of LA/LAA thrombus in patients with non-valvular atrial fibrillation. The pre⁃ dictive values of both score systems are moderate. There are also LA/LAA thrombus in low-moderate risk patients when us⁃ ing the two score systems, and with the increased risk stratification, the rate of LA/LAA thrombus is increased. Related Articles | Metrics

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Analysis of results of arthrodesis in 30 patients with severe hallux rigidus ZHAO Xiaotao, GAO Xiang△, ZHANG Dianying, YU Kai, DONG Shengli, LI Jianpeng 2015, 43 (3):  308-310.  doi: 10.11958/j.issn.0253-9896.2015.03.023 Abstract ( 700 )   PDF (844KB) ( 4069 )   Objective To observe the clinical effect of arthrodesis in treating severe hallux rigidus. Methods Totally 30 patients (30 feet) with severe hallux rigidus treated by arthrodesis from January in 2010 to January in 2013 were retrospec⁃ tively analyzed. AOFAS and VAS scoring system were employed to evaluate the clinical curative effect including acquiring the fusion rate, the time to fusion and the incidence rate of hallux valgus. Forefoot-plantar pressure values before and after operation were measured by Plantar pressure measurement system. Results The mean follow-up time was 18 months. AO⁃ FAS score was raised from (48.2±7.6) to (72.8±6.2) after operation (P＜0.01). VAS score was also increased from (1.7±0.7) to (8.3±1.1) after operation (P＜0.01). The average healing time of knitting was 12.1 weeks. None was non-union and devel⁃ oped to hallux valgus. The forefoot-plantar pressure returned to normal postoperatively from abnormal laterally distribution. Conclusion The surgical strategy of arthrodesis is proved to be effective in treating severe hallux rigidus, in terms of capa⁃ bility of relief from pain, recovery of forefoot-plantar pressure distribution, low incidence of complication. Related Articles | Metrics

Epidemiological Investigation

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Knowledge, attitudes and practices of combined oral contraceptives in Tianjin obstetrics and gynecology personnels HUO Yan, ZHANG Yujuan, WANG Jianmei△, YANG Jing, XIE Guohua 2015, 43 (3):  311-314.  doi: 10.11958/j.issn.0253-9896.2015.03.024 Abstract ( 716 )   PDF (338KB) ( 3979 )   Objective To understand the knowledge, attitude, practice (KAP) status and relative demand to combined oral contraceptives (COCs) in medical staffs of obstetric and gynecological department in Tianjin, providing reference basis for the effective intervention measures. Methods By using random, stratified and clustered sampling approach, we selected 382 medical staffs of obstetric and gynecological department in Tianjin. A self-administered Questionnaire on COCs KAP was employed to survey the general characteristics, awareness of COCs knowledge, attitudes to COCs, service capabilities of contraceptive counseling, prescribing behavior and request of continuing education. Results The respondents showed defects in COCs indications, non- contraceptive benefits, relationship with cancer and birth defects. The average knowledge score was higher in doctors than that of nurses. The higher the titles and qualifications, the higher the knowledge score. A to⁃ tal of 90.11% of respondents believed that strengthening the contraceptive guidance can help to reduce pregnancy. Strength⁃ ening the interpretation of misunderstanding (81.04% ) and enhancing the treatment and follow- up of adverse reactions (62.09%) can help to reduce stop taking. Only 25.82% of respondents could provide detailed COCs relative consulting servic⁃ es; 42.96% of obstetricians and gynecologists refused prescribing COCs for women over the age of 40 for the purpose of con⁃ traception. A total of 93.13% medical personnel showed demand on relative training. Conclusion It’ s necessary to perform continuing education to medical staffs via different intervention, so as to improve their cognitive level to COCs, ameliorate COCs relative attitudes and behavior, and help them providing better service to women of childbearing age. Related Articles | Metrics

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Correlation of carotid intima-media thickness and metabolic syndrome in Tianjin city policemen LIU Wen， LIU Xuan， YUE Song 2015, 43 (3):  315-318.  doi: 10.11958/j.issn.0253-9896.2015.03.025 Abstract ( 723 )   PDF (371KB) ( 3849 )   Objective To analyze the relationship between carotid intima-media thickness (CIMT) and metabolic syndrome (MS), and explore risk factors for CIMT incrassation in Tianjin policemen. Methods Fifty MS patients (MS group) and 50 healthy people (control group) in health examination of Tianjin policemen were enrolled in this study. The question⁃ naire survey(family history， past medical history， present history， living habits and occupational stress factors) were recorded in two groups of patients. The blood indicators (fasting plasma glucose, triglycerides, total cholesterol, high density lipopro⁃ tein cholesterol, low density lipoprotein cholesterol, homocysteine) were detected. The color Doppler was used to measure CIMT incrassation, and incrassation ratio was calculated. The relative indicators were compared between two groups. The logistic regression model was used to analyse the influencing factors of carotid artery intima-media thickness. Results CIMT and CIMT incrassation ratio (communis, internal and external carotid) were significantly higher in MS group than those of control group (P＜0.01). The risk factors for CIMT included family history of hypertension, chronic stress, smoking, family history of diabetes, high fat diet and high homocysteine levels, and dairy products were protective factors for CIMT incrassa⁃ tion. Conclusion The reason of carotid artery intima-media thickness is diversity. The healthy lifestyle and clinical intervention to retard CIMT are advocate, which are conducive to the control of atherosclerosis. Related Articles | Metrics

Diagnostic Techniques

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The diagnostic value of ultrasound elastography and acoustic radiation force impulse imaging in solitary solid thyroid nodule DU Tingting， LI Jun， XIA Dongqin， LU Guilin 2015, 43 (3):  319-322.  doi: 10.11958/j.issn.0253-9896.2015.03.026 Abstract ( 778 )   PDF (856KB) ( 3818 )   Objective To evaluate the diagnostic values of ultrasound elastography (UE) technology and acoustic radia⁃ tion force impulse (ARFI) technology in the differential diagnosis of benign and malignant single solid thyroid nodule showed by ultrasonography. Methods A total of 100 patients with solitary thyroid nodule diagnosed by the Affiliated Hospital, School of Medicine of Shihezi University in December 2013 to July 2014 were selected in this study. The routine ultrasound, UE examination and ARFI technology were used in patients before operation. All patients were performed operation for thy⁃ roid nodules and the diagnosis was confirmed by the pathologic results. A flexible classification method was used in UE. ARFI was used to detect shear wave velocity (SWV) of lesions. The diagnostic values of three methods were evaluated by the gold standard of pathologic results. ROC curves were plotted according to SWV values of benign and malignant thyroid nod⁃ ules. Results The area under the curve (ACU) was 0.960. The best cut-off value of SWV was 2.76 for diagnosis. The diag⁃ nostic sensitivities of malignant thyroid solid nodules were 82.61% (19/23), 82.61% (19/23) and 91.30% (21/23) for US, UE and ARFI, respectively. The diagnostic specificities were 87.01% (67/77), 88.31% (68/77) and 93.51% (72/77) respectively. And the positive predictive values (PPV) were 65.52% (19/29), 67.86% (19/28) and 80.77% (21/27) respectively； the negative predictive values (NPV) were 94.37% (67/71)， 94.44% (68/72) and 97.29% (71/73). Conclusion ARFI technology is superior to US and UE technology in predicting malignancy in solitary solid thyroid nodule, which is worth of clinical applica⁃ tion and promotion. Related Articles | Metrics

Review

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Research status on Ts-SV40-mediated temperature sensitive cells LIAO Yuping, LI Xiaohong, SUN Hongtao 2015, 43 (3):  323-326.  doi: 10.11958/j.issn.0253-9896.2015.03.027 Abstract ( 525 )   PDF (336KB) ( 4097 )   The in vitro passage number and proliferation of non-immortalized cells are limited, which restrictions cell therapy or in vitro studies. Cells transfected with temperature sensitive simian virus 40 large T antigen (ts-SV40LT) gene could show the greatest proliferation. The cells can be amplified with compensating the lack of limited number of cells under the permissive temperature. Non-permissive temperature can be used in studying the cell therapy or its other physiological characteristics. This research field involves peritoneal stromal cells, satellite cells of urinary tract, oral epithelial cells, adre⁃ nal medullary cells, bone marrow-derived endothelial cells, retinal progenitor cells, mesenchymal stem cells, hematopoietic stem cells, mast cells, podocytes and Kupffer cells. In this study, the current research on Ts-SV40-mediated temperaturesensitive cells was reviewed. Related Articles | Metrics

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Advances on the relationship between thrombin-activatable fibrinolysis inhibitor (TAFI) and recurrent pregnancy loss YI Xiaofang, TAN Chao 2015, 43 (3):  327-329.  doi: 10.11958/j.issn.0253-9896.2015.03.028 Abstract ( 657 )   PDF (284KB) ( 4681 )   Recurrent pregnancy loss (RPL) is a common complication of pregnancy, which affects 2% fertile women. A recent research has found that high level of thrombin-activatable fibrinolysis inhibitor (TAFI) can reduce the occurrence risk of early RPL. TAFI is one kind of carboxypeptidase, which can be activated as TAFIa. TAFIa can make the fibrinolysin lose its working site, which can interact with the fibrin to play a role in the regulation of fibrinolysis and the inhibition of throm⁃ bus formation. The damage of fibrinolytic system is one of the risk factors for the occurrence of RPL in pregnant women, which has become one of the hotspots in the medical profession. In this paper, recent literature on TAFI and its relationship with recurrent pregnancy loss has been reviewed, hoping for new ways and clues in clinical treatment and prevention of RPL. References | Related Articles | Metrics

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Role of haemophilus influenza factor in the process of biofilm formation GAO Xue1, SHANG Xiaoling2, ZHANG Yutuo1△ 2015, 43 (3):  330-332.  doi: 10.11958/j.issn.0253-9896.2015.03.029 Abstract ( 610 )   PDF (321KB) ( 3997 )   The Haemophilus influenzae (Hi) is a common opportunistic pathogen from human respiratory tract. The lower immunity can cause the disease. After the biofilm (BF) is formatted by Hi at the site of infection, the antibiotic sensitivity is declined, which often causes the chronic disease, inducing the difficulty in clinical treatment. In recent years, the pathogenicity of Hi BF and BF form related factors have gradually become the focus of clinical and basic research. This paper reviewed recent studies as following. Related Articles | Metrics

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Advances of vascular endothelial growth factor inhibitors in the treatment of psoriasis LI Junying， LI Hong 2015, 43 (3):  333-336.  doi: 10.11958/j.issn.0253-9896.2015.03.030 Abstract ( 730 )   PDF (301KB) ( 4059 )   Psoriasis is a common inflammatory autoimmune disease. Angiogenesis is known to be a key pathogenic fea⁃ ture of psoriasis. The elevation of vascular endothelial growth factor (VEGF) has been demonstrated in the skin and plasma of patients with psoriasis. A number of case reports have indicated that VEGF inhibitor is effective in patients with psoriasis. VEGF inhibitors are consisted of three categories: anti-VEGF monoclonal antibodies, VEGF receptor antagonists and tyro⁃ sine kinase inhibitors. This article reviewed the current clinical application and therapeutic potential of VEGF inhibitors in psoriasis . Related Articles | Metrics