Abstract: 【Abstract】 Objective To investigate the relationship between gene polymorphisms of leptin receptor and the serum level of lipids、LEPR、hypertension. Methods 170 subjects with EH and 77 controls without EH were involved in this study. serum LEPR level was measured by ELISA. The LEPR A109G and LEPR A223G genotypes were determined by polymerase chain reaction （PCR）and restriction fragment length polymorphism （RFLP）. Results A significant differences in genotypes and allele frequencies of A109G、A223G polymorphisms were detected between EH subjects and controls. The plasma levels of TC and TG in subjects with A allele of the A109G were higher than subjects with G allele（p<0.05）.The level of lipids was not affected by A223G polymorphism. LEPR level in the subjects with A allele was lower than the subjects with G allele. Conclusions LEPR gene mutation in 109 site and 223 site of exon 4 and 6 may be related with the EH. There was an obvious relationship within LEPR A109G mutation and high TC and TG level. The mutation A allele of 223 site shows lower levels of LEPR .

Key words: PCR—RFLP, polymorphism, EH, LEPR