Abstract: Abstract Objective:To invstigate the frequency distribution of VNTR-ZNF ACCCC inserted and deleted polymorphism in the promoter region of ATP-binding cassete Transporter 1(ABCA1) and the association with plasma level of high-density lipoprotein (HDL). Methods: A total of 265 cases were recruited from Tianjin district,including166 patients with coronary heart disease (CHD) and 99 healthy donors. The corresponding DNA fragments were amplified with PCR and the genotypes were separated by polyarcrylamide gel electrophoresis. Results:the allele frequences of inserted form and deleted form of VNTR-ZNF were 0.325,0.714,and the genotypic frequencies of VNTR-ZNF were 6.7% for the inserted form ,45.5% for the deleted form and 51.8% for the inserted/ deleted form,but the frequency distribution of allele and genotype didn’t significantly differ between CHD and contol groups and there were no significant differences of HDL level in three different genotypes .Conclusion: the frequency of deleted form of VNTR-ZNF in ABCA1 in Tianjin district was markedly higher than that in European district ,but the variation of ABCA1 VNTR-ZNF was not associated with plasma level of HDL.

Key words: ATP-binding cassete Transporter 1, high-density lipoprotein, polymorphism