Abstract:

[Abstract]   Objective   To study the function of the nano-molecular polyamide-amine (PAMAM) as microRNA(miR)
carrier targeting gastric adenocarcinoma, and the foundation of developing an efficient delivery of small molecule drugs targeting gastric cancer thereof.  Methods   Thefolic acid (FA) /PAMAM comoles compound was prepared by dialysis method. After transfection of miR-7or liposomes into SGC-7901cell line, fluorescence microscope was used to detect the gene transfect efficiency. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the miR-7level. The immunocytochemistry assay was used to test the protein expressions of epidermal growth factor receptor (EGFR), protein kinase B (PKB) and proliferating cell nuclearantigen (PCNA). The transwell system was utilized to explore the migration ability of tumor cells.  Results   Compared with liposme, FA/PAMAN complex compound can significantly improve the level of miR-7 in SGC-7901cells，reduce the protein levels of EGFR, PKB and PCNA in SGC-7901cells, and also reduce the percentage of cancer cell migration (P＜0.05).   Conclusion   PAMAM can effectively transfect miR into gastric cancer cells, which is expected to become an efficient delivery of small molecule drugs.

Key words: gastric adenocarcinoma, liposomes, microRNA, nanomaterials, polyamide-amine