Abstract:

[Abstract]   Objective   To study the influence of qiqishen granules on the cellular and humoral immune functions in
model mice.  Methods   Six-week-old mice were divided into control group and qiqishen granule (high, medium and lowdose) groups. The suspension of chicken red blood cells was injected into the mouse abdominal cavity. The influence of qiqishen granules on the phagocytic function of the macrophages in mouse abdominal cavity was observed. The sheep red blood cells (SRBC) were prepared. The blood corpuscle coagulation was observed, and the serum hemolysin was detected. The activity of the mouse natural killer (NK) cells were detected by the interaction between the target cell (YAC-1) and spleen cell (the response cell). The influence of qiqishen granules on the cellular immunity was detected by the lymphocyte transforming assay. The influence of qiqishen granules on organ/body weight ratio was measured by calculating the thymus/body and spleen/body ratio.    Results    Compared with the control，qiqishen granules significantly improved phagocytic function of the macrophages in mouse abdominal cavity. The humoral immune function was also improved in mice. The activity of NK cell was enhanced by qiqishen granules, which also enhanced the lymphocyte transforming induced by the concanavalin (Con) A. There was no significant influence in the organ/body weight ratio.   Conclusion   The qiqishen granules could increase the immunomodulating effect, indicating it would have a good using prospect．

Key words: qiqishen granules, immunity, cellular, humoral immunity, phagocytosis, hemolysin proteins, killer cells, 天然