Tianjin Medical Journal

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Effects of Programmed Cell Death 4 Gene on Expression of Death Associated Protein 5 and Apoptosis in Colon Cancer Cells

YI Bo 1,LI Qiyun 1,RAO Huamin 2,SHAO Jianghua 1   

  1. 1. Department of Abdominal Surgery，Jiangxi Province Tumor Hospital
    2. Department of Abdominal Surgery.Jiangxi Province Tumor Hospital
  • Received:2012-07-11 Revised:2013-01-10 Published:2013-06-15 Online:2013-06-15
  • Contact: YI Bo

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Abstract: [Abstract]   Objective   To investigate the effect of exogenous programmed cell death4(PDCD4) gene on the expres-
sion of death associated protein5(DAP5) and apoptosis of human colorectal cancer cell LOVO, and involved mechanisms thereof.   Methods   Recombinant eukaryotic plasmid pFLAG/PDCD4was constructed and transfected into human colorectal cancer cell LOVO. Cells stably expressing PDCD4were established by G418selection (500mg/L). The levels of PDCD4protein and mRNA were analyzed by RT-PCR and Western blotting. The apoptotic cells were measured by flow cytometry, and protein expression of DAP5was detected by Western blotting.  Results LOVO-pFLAG/PDCD4cell line was successfully es-tablished by G418selection. Compared to non-transfection and mock-transfection group, the levels of PDCD4mRNA and protein were significantly increased, the cell apoptosis ratio was enhanced and the expression of DAP5protein was increased in transfection group (P<0.01).   Conclusion PDCD4could induce apoptosis of human colorectal cancer LOVO cells, which mechanism might be involved in up-regulating DAP5protein.

Key words: protein kinases, colorectal neoplasms, carcinoma, cell cycles, Plasmids, transfention, recombination, 遗传