Abstract:

[Abstract] Objective To observe the relationship between expression changes ofγH2AX and the radiosensitivity of lung cancer cells in vitro.Methods The radiosensitivity of lung cancer cell lines A549and SBC-3was measured by clone forming assay. The DSBs damage of lung cancer cell lines A549and SBC-3was determined by Western blot assay.Re? sults The clone forming rates of lung cancer cell lines A549and SBC-3were gradually decreased with the increased radia? tion dose. γH2AX expression was related to the cell radiosensitivity1hour and6hours after radiated. Conclusion The phosphorylated histoneγH2AX is a powerful tool to monitor DNA DSBs and to predict the radiosensitivity in lung cancer ra? diotherapy.

Key words: γH2AX protein, lung cancer, radiosensitivity