Abstract:

[Abstract] Objective To study the IE and pp67 mRNA expressions in infants infected human cytomegalovirus
(HCMV) after ganciclovir treatment. Methods  The values of total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were detected in patients with HCMV before treatment, 2 weeks and 4 weeks after treatment. The HCMV-DNA was detected by quantitative PCR. The expression levels of IE mRNA and pp67 mRNA were detected by real-time fluorescent quantitative PCR. Results HCMV DNA stopped copying and the infected in fants were cured following the traditional criterion, however, RNA expression was still tested in part of these infants. Conclusion It should be identified for mRNA expression when HCMV-DNA copies were hardly identified in these infants to prevent from relapse.

Key words: cytomegalovirus, infant, Ganciclovir, IE mRNA, pp67 mRNA, virus identification