Abstract:

Abstract: Objective To observe the effects of Hedan tablet on cytokines and oxidation factors in APOE-/- mouse, and
to explore its effect on atherosclerosis and to explore its behind mechanism. Methods APOE-/- mice (n=50) were randomly
divided into control group, model group, low dose Hedan tablet treatment group, high dose Hedan tablet treatment group and
simvastatin treatment group. Mice in control group were given normal feed while mice in other groups were fed with high cho⁃
lesterol diet. Hedan or Simvastatin was administrated intra-gastrically while normal saline was given to model group in the
same route. After 12 weeks, mice were sacrificed to observe the mRNA level of tumor necrosis factor-α(TNF-α mRNA) in
aorta by RT-PCR. Mean while, serum levels of interleukin-1 (IL-1), interleukin-10 (IL-10), malonaldehyde (MDA) and su⁃
peroxide dismutase (SOD) were determined in different groups. Results Compared with control group, TNF-α mRNA tran⁃
scription level as well as serum levels of IL-1 and MDA significantly increase while serum levels of IL- 10 and SOD de⁃
creased remarkably in model group, (P < 0.01). Compared with model group, mRNA levels of TNF-α as well as serum levels
of IL-1 and MDA were significantly decreased while serum levels of IL-10, SOD were greatly increased in low dose and high
dose Hedan tablet treatment groups as well as in simvastatin treatment group (P < 0.01). Conclusion Hedan tablet inhibit
the formation of atherosclerosis through its anti-oxidation role and anti-inflammation role.

Key words: atherosclerosis, apolipoproteins E, models, animal, Hedan tablet, atherosclerosis, APOE-/- mouse, inflam?
mation factor, oxidation stress factor