Abstract: Abstract: Objective A serum metabolic profiling in acute myocardial infarction (AMI) rat was established to screen
potential metabolic markers of AMI prognosis and complication. Methods Male Wistar rats（n=20）were divided randomly
into AMI 1 week group and control group. Anterior descending coronary was ligated in rats in AMI 1 week group to establish
AMI model. After one week, these rats were sacrificed and the blood was collected from heart. And metabolomics platform
was used to analyze serum metabolic profile. After PCA (principal component analysis) and OPLS-DA (Orthogonal partial
least squares - Discriminant Analysis) were established, SPSS 19.0 was used to analysis data to get the potential metabolic
markers. Results The PCA and OPLS-DA were established successfully and 27 metabolites present differently in levels
between AMI 1 week group and the control group. Among these metabolites, LysoPC (lysophosphatidylcholine) and LysoPE
(lysophosphatidyl ethanolamine) demonstrate significant differeance between these two groups. Conclusion The metabolic
disorder in AMI patients can be reflected from the serum metabolic profiling. And these significant metabolites provide sup⁃
port and reference for the prevention of AMI complication and its treatment.

Key words: myocardial infarction, rats, Wistar, chromatography, high pressure liquid, mass spectrometry, serum metabolomics