Abstract: Objective To study the effect of curcumenol on the inflammatory factors in peritoneal fluid of rats with endometriosis (EMS), and to explore its therapeutic mechanism. Methods Adult female SD rats were used to prepare EMS model by autotransplantation. The EMS model rats were randomly divided into model group and curcumenol group (20 mg/ kg), and another sham operation group was set up, 10 rats in each group. Rats in each group were given the drug by intragastric administration once a day for four weeks. After the last administration, the rats were anesthetized, and samples of the abdominal fluid were collected. Pathological morphology of ectopic endometrium in rats was observed. The contents of monocyte chemotactic protein-1 (MCP-1), macrophage migration-inhibitory factors (MIF), tumor necrosis factor-alpha (TNF)-α, interleukin (IL)-1β and IL-6 in peritoneal fluid were detected. The TNF-α expression in endometrial tissue was detected by immunohistochemistry, and the fibroblast growth factor 2 (FGF-2) mRNA expression was detected by real-time PCR. Results Compared to the model group, the ectopic endometrium atrophy was found in curcumenol group, the contents of MCP-1, MIF, TNF - α, IL-1β, IL-6 in the peritoneal fluid of rats and the TNF - α expression, the FGF-2 mRNA expression in endometrial tissues were significantly decreased in curcumenol group (P＜0.05). Conclusion The curcumenol can inhibit the inflammatory reaction in the peritoneal microenvironment, which may be related to its treatment effect on EMS.

Key words: endometriosis, Curcumol;, chemotactic factors, macrophage migration-inhibitory factors, fibroblast growth factor 2;, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6