Protective effect of asiatic acid on brain injury after experimental subarachnoidhemorrhage in rats

doi:10.11958/20231956

Abstract

Abstract:

Objective To explore the neuroprotective effect of asiatic acid (AA) on brain damage after experimental subarachnoid hemorrhage (SAH) in rats. Methods A total of 108 adult SD rats were divided into the sham1 group, the SAH+vehicle group and the SAH+AA group, with 36 rats in each group. The 42 rats were divided into the sham2 group, 3, 6, 12, 24, 48 and 72 h after SAH groups, with 6 rats in each group. Except the sham group, SAH model was established by unilateral external carotid artery puncture method in other groups. After modeling, the SAH+AA group was given AA solution (30 mg/kg) by gavage. Neurobehavioral changes were assessed by foot fault test and modified Garcia score. Western blot assay was used to detect the protein level of glutathione peroxidase 4 (GPX4) in brain tissue. ELISA was used to detect the concentrations of glutathione (GSH) and malondialdehyde (MDA). Fluoro Jade B (FJB) staining was used to detect the neuronal death. Results Compared with the sham1 group, the SAH+vehicle group showed a significant increase in the proportion of empty steps and a significant decrease in the modified Garcia score, a significant decrease in GPX4 protein levels, a significant increase in MDA concentration (P<0.05), a decrease in GSH concentration (P<0.01) and a significant increase in the number of dead neurons (P<0.05). Compared with the SAH+vehicle group, a significant decrease in the proportion of empty steps, a significant increase in the modified Garcia score, a significant increase in GPX4 protein level, a significant decrease in MDA concentration, a significant increase in GSH concentration (P<0.05) and a significant decrease in the number of dead neurons in the SAH+AA group (P<0.05). Conclusion AA may reduce brain injury after SAH in rats by inhibiting lipid peroxidation.

Key words: asiatic acid, lipid peroxidation, subarachnoid hemorrhage, brain damage

CLC Number:

R743.35

Figures/Tables 6

References 20

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组别	n	造模前	SAH后3 d	SAH后5 d	SAH后7 d	SAH后14 d
Sham1组	12	1.58±0.90	1.50±1.45	1.25±0.97	0.75±0.75	0.67±0.89
SAH+Vehicle组	12	1.50±1.31	18.42±1.56^a	15.33±1.44^a	12.83±1.59^a	8.67±1.61^a
SAH+AA组	12	1.67±0.98	8.75±1.91^b	6.25±1.42^b	4.91±1.88^b	3.00±0.85^b
F		0.071	316.300^＊＊	365.900^＊＊	204.800^＊＊	147.900^＊＊

组别	n	造模前	SAH后3 d	SAH后5 d	SAH后7 d	SAH后14 d
Sham1组	12	1.58±0.90	1.50±1.45	1.25±0.97	0.75±0.75	0.67±0.89
SAH+Vehicle组	12	1.50±1.31	18.42±1.56^a	15.33±1.44^a	12.83±1.59^a	8.67±1.61^a
SAH+AA组	12	1.67±0.98	8.75±1.91^b	6.25±1.42^b	4.91±1.88^b	3.00±0.85^b
F		0.071	316.300^＊＊	365.900^＊＊	204.800^＊＊	147.900^＊＊

组别	n	造模前	SAH后3 d	SAH后5 d	SAH后7 d
Sham1组	12	17.8±0.5	17.5±0.5	17.6±0.5	17.6±0.5
SAH+Vehicle组	12	17.5±0.5	12.3±1.4^a	13.3±1.6^a	14.1±1.6^a
SAH+AA组	12	17.6±0.5	14.9±0.7^b	15.8±0.7^b	16.6±0.8^b
F		0.786	90.460^＊＊	51.090^＊＊	35.020^＊＊

组别	n	造模前	SAH后3 d	SAH后5 d	SAH后7 d
Sham1组	12	17.8±0.5	17.5±0.5	17.6±0.5	17.6±0.5
SAH+Vehicle组	12	17.5±0.5	12.3±1.4^a	13.3±1.6^a	14.1±1.6^a
SAH+AA组	12	17.6±0.5	14.9±0.7^b	15.8±0.7^b	16.6±0.8^b
F		0.786	90.460^＊＊	51.090^＊＊	35.020^＊＊

组别	n	GPX4	MDA/（μmol/g）	GSH/（mg/g）
Sham1组	6	1.00±0.11	5.34±0.51	9.27±0.92
SAH+Vehicle组	6	0.57±0.06^a	10.77±1.53^a	6.93±1.06^a
SAH+AA组	6	0.71±0.09^b	7.10±0.46^b	8.26±0.92^b
F		37.870^＊＊	49.050^＊＊	10.260^＊＊