Moderately elevating blood potassium concentration can improve mitochondrial function of brain tissue in cardiopulmonary resuscitation rats

doi:10.11958/20221699

Abstract

Abstract:

Objective To explore whether moderately hyperkalemia can improve mitochondrial function of brain tissue during cardiopulmonary resuscitation (CPR) in rats. Methods Eighty rats were randomly divided into five groups: the sham (SH) group, the normal saline (NS) group, the low dosage of potassium chloride (LK) group, the mid-dosage of potassium chloride (MK) group and the high-dosage of potassium chloride (HK) group, with 16 rats in each group. Cardiac arrest (CA) /CPR model was established by transesophageal electrical stimulation to induce ventricular fibrillation. Rats were received equal volume of normal saline and the corresponding dose of potassium chloride via intravenous injection when CPR was initiated at 6 min after CA. The mean arterial pressure (MAP) and the blood potassium concentration were detected after return of spontaneous circulation(ROSC). The neurological deficit scores (NDS), the changes of cell morphology of hippocampal tissue, the content of adenosine-triphosphate, the activity of Na⁺/K⁺-ATP enzymes, the respiratory chain complex (Ⅰ/Ⅱ/Ⅲ/Ⅳ) of the hippocampal tissue at 24 h post ROSC were recorded and observed between different groups. Results Compared with the NS group, the blood potassium was significantly higher in the MK group and the HK group at 1 min and 10 min post ROSC (P<0.05). Compared with the NS group, the MAP after ROSC was not significantly affected, and the NDS was significantly increased at 24 h after ROSC in the MK group (P<0.05). Compared with the NS group, the MAP within 8 min after ROSC was significantly decreased (P<0.05), and the NDS was not significantly improved at 24 h post ROSC in the HK group. Compared with the NS group, the activity of hippocampal respiratory chain complex Ⅲ was increased in the LK group, and the content of ATP was increased (P<0.05). Compared with the NS group, the activity of respiratory chain complex (Ⅰ/Ⅱ/Ⅲ), the content of ATP and the activity of Na⁺/K⁺-ATP enzyme at 24 h post ROSC were increased in the MK group (P<0.05), and hippocampal cell morphology was improved 24 h after ROSC. Compared with the NS group, the activity of respiratory chain complex Ⅲ increased in the HK group (P<0.05). The MK group showed the most obvious improvement in each evaluation index. Conclusion Moderately elevating blood potassium at onset of CPR can improve mitochondrial function and alleviate cerebral ischemia -reperfusion injury in CPR.

Key words: potassium chloride, cardiopulmonary resuscitation, reperfusion injury, mitochondria, electron transport, complex

CLC Number:

R345

Figures/Tables 8

References 13

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组别	体质量/g	心率/（次/min）	MAP/mmHg	电刺激时间/s
SH组	222.13±9.51	390.88±16.20	93.19±7.17
NS组	225.13±9.05	393.81±22.98	92.44±7.81	61.75±2.38
LK组	223.38±7.77	384.94±14.22	93.31±6.34	61.44±2.25
MK组	222.75±6.05	389.56±16.86	90.75±5.61	61.88±3.05
HK组	224.31±8.93	394.25±17.94	94.13±7.32	61.13±2.06
F	0.330	0.711	0.546	0.298

组别	体质量/g	心率/（次/min）	MAP/mmHg	电刺激时间/s
SH组	222.13±9.51	390.88±16.20	93.19±7.17
NS组	225.13±9.05	393.81±22.98	92.44±7.81	61.75±2.38
LK组	223.38±7.77	384.94±14.22	93.31±6.34	61.44±2.25
MK组	222.75±6.05	389.56±16.86	90.75±5.61	61.88±3.05
HK组	224.31±8.93	394.25±17.94	94.13±7.32	61.13±2.06
F	0.330	0.711	0.546	0.298

组别	ROSC 只数	ROSC 时间/s	ROSC后24 h 生存只数	ROSC 后24 h NDS/分
SH组	0		16	80.00±0.00
NS组	10	92.70±14.28	9	72.11±1.45^a
LK组	12	83.00±10.97	12	73.58±1.68
MK组	15	82.53±12.87	15	76.40±1.35^bc
HK组	10	98.60±15.47^cd	9	72.67±2.23^d
F		3.927^＊		68.534^＊＊

组别	ROSC 只数	ROSC 时间/s	ROSC后24 h 生存只数	ROSC 后24 h NDS/分
SH组	0		16	80.00±0.00
NS组	10	92.70±14.28	9	72.11±1.45^a
LK组	12	83.00±10.97	12	73.58±1.68
MK组	15	82.53±12.87	15	76.40±1.35^bc
HK组	10	98.60±15.47^cd	9	72.67±2.23^d
F		3.927^＊		68.534^＊＊

组别	n	2 min	4 min	6 min	8 min	10 min	30 min	60 min
SH组	16	87.88±3.84	86.81±3.94	87.06±4.33	87.25±3.73	87.00±4.05	87.56±3.37	87.81±3.80
NS组	9	72.90±2.51^a	74.60±3.03^a	75.70±1.83^a	77.00±2.10^a	77.40±2.95^a	78.50±3.24^a	78.40±3.60^a
LK组	12	78.33±5.21^b	78.83±5.37	79.42±5.52	80.50±4.78	80.58±4.62	80.75±5.03	80.75±5.85
MK组	15	73.60±2.97	73.93±2.52	74.93±2.74	75.93±3.61	77.13±3.87	78.27±4.53	78.40±4.82
HK组	9	58.90±4.46^bcd	61.00±4.57^bcd	62.80±4.76^bcd	66.00±6.68^bcd	71.30±8.54	73.80±7.70^c	75.70±6.89
F		89.356^＊＊	68.531^＊＊	57.534^＊＊	39.473^＊＊	17.440^＊＊	14.489^＊＊	11.805^＊＊