Abstract: Objective:To investigate the effect of TNF–αin the pathophysiological course of obesity-inflammation-insulin resistance(IR)-type 2 diabetic mellitus(T2DM).Methods: 3T3-L1 preadipocytes were cultured and induced into mature adipocytes.Observed the expressions of ADPN mRNA,PPAR-γ mRNA in the differential course of the adipocytes. Observed the expressions of ADPN mRNA,PPAR-γ mRNA, TNF–αmRNA, IL-6 mRNA and IL-1β mRNA in mature adipocytes which were treated with high concentration （20ng/mL）of the recombinant TNF–αunder diverse phase.Results:The levels of ADPN mRNA,PPAR-γ mRNA expressions were upregulated gradually within the differential course of 3T3-L1 adipocytes.The results of the genes’ expressions in adipocytes treated by TNF–α（20ng/mL）were as following: The expressions of ADPN mRNA and PPAR-γ mRNA decreased with time-dependent manner.The expression of TNF–αmRNA and IL-6 mRNA increased significantly. The expression of IL-1β mRNA didn’t show change in statistics.Conclusions: The inflammation hypothesis on etiologic mechanism of type 2 DM was studied at molecular level in this work, TNF–αplays an important role in the course of obesity-inflammation-IR-T2DM.

Key words: tumor necrosis factor-α , 3T3-L1 adipocytes , peroxisome proliferator-activated receptor gamma , adiponectin , interleukin-6 , interleukin-1β, insulin resistance, 肥胖症