Abstract:

[Abstract] Objective  To investigate the effect of LY294002 on proliferation of cultured human glomerular mesangial cells, and to study on the mechanism underlying this proliferation. Methods  Different concentrations of LY294002 (0.02，0.2，2，20，100 mg/L) were administrated to the cultured human glomerular mesangial cells. The effects of mesangial cell proliferation were measured using CCK-8 colorimetric assay. Under appropriate concentrations, proliferation of cultured mesangial cells were measured using CCK-8 at 0.5, 1, 24, 48 hours of drug administration time. Results  LY294002（0.02 mg/L）didn’t obviously inhibited the growth of mesangial cells（P＞0.05）, the inhibition rate was 5.05%. The effect of LY294002 on the cells decreased with rising concentration (0.2 to 20 mg/L) in a dose-dependent manner (P<0.05). Mesangial cells would stop growing with the increasing concentration. LY294002 didn’t obviously inhibited the growth of mesangial cells at 2 mg/L during 0 to 1 h（P＞0.05）, but inhibited proliferation gradually from 1 to 48 h (P < 0.05). Conclusion  Within the certain range of concentration and time, the LY294002 inhibits the proliferation of human glomerular mesangial cells, and PI3K/Akt/mTOR signaling pathway may regulate the proliferation of human mesangial cell.

Key words: 1-phosphatidylinositol 3-kinase, mesangial cells, PI3-Kinase-Akt-mTOR pathway, cell proliferation, LY294002