Abstract:

[Abstract] Objective  To explore the inhibitory effect of fructus corni polysaccharides on human cervical cancer cells and expressions of Survivin and Bcl-2 in HeLa cells. Methods  MTT assay was used to determine the cell proliferation. Flow cytometry and immunohistochemistry SP methods were used to detect the apoptosis and protein expressions of Survivin and Bcl-2 in HeLa cells. Results  There was a significant inhibitory effect of fructus corni polysaccharides on the proliferation in HeLa cells. The inhibitory degree was correlated with different concentrations and different action times of fructus cornipolysaccharides. The apoptosis rates of HeLa cells were 10.1%, 21.6% and 28.9% for fructus corni polysaccharides concentrations of 6.25 g/L, 12.5 g/L and 25 g/L. The expressions of Survivin and Bcl-2 in HeLa cells were significantly lower in fructus corni polysaccharides treatment group than those of control group. Conclusion  Fructus corni polysaccharides showed significant inhibitory effects on the proliferation in HeLa cells, which also decreased Survivin and Bcl-2 expressions and induced the cell apoptosis.

Key words: Fructus Corni poLysaccharides, Cervical Cancer, Hela, Survivin, Bcl-2, inhibitory, Apoptosis