Tianjin Medical Journal

Tianjin Med J ›› 2015, Vol. 43 ›› Issue (12): 1356-1360.doi: 10.11958/j.issn.0253-9896.2015.12.004

• Cell and Molecular Biology • Previous Articles     Next Articles

Effects of ZHX3 gene silence on the expression of osteoblast-related factors in BMSCs

ZHANG Miaomiao1, BAO Cuifen2, WANG Yan3, MIN Heming4 , QIN Shujian1△   

  1. 1 Department of Human Anatomy and Histology and Embryology, 2 Key Lab of Molecular Cell Biology and New Drug Development, 3 Department of Neurology, First Affiliated Hospital of Jinzhou City; 4 Department of Cell Biology, Liaoning Medical University, Jinzhou 121000, China
  • Received:2015-08-05 Revised:2015-09-15 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail：mianyizuhua@aliyun.com E-mail:1538702627@qq.com

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Abstract: Objective To investigate the effects of zinc fingers and homeoboxes 3 (ZHX3) silence on expressions of smad3, smad4 and RUNX2 in bone marrow mesenchymal stem cells (BMSCs). Methods ZHX3 low expression vector (ZHX3 silent group) was constructed and was transfected to rat BMSCs. Empty vector was transfected into BMSCs and was used as vehicle control group, and wild type BMSCs was used as the control group. The cell transfection rate was measured under a fluorescence microscope, and then the successful transfection was identified. The immunocytochemistry and immu⁃ noblotting methods were used to detect the expression levels of smad3, smad4 and RUNX2. Results (1) Cells with BMSCs phenotype can be obtained by recovery culturing. (2) After transfection, the green fluorescent protein was found in ZHX3 si⁃ lence group and vehicle control group. Blank control group showed no significant fluorescence. The expression level of ZHX3 was significantly lower in ZHX3 silence group than that of vehicle control group. (3) Results of immunofluorescence asssay showed that the positive expressions of smad3 and smad4 were located in nucleus and cytoplasm, the positive expression of RUNX2 was mainly located in nucleus. Positive cells were observed in three groups. There was no significant difference in fluorescence intensity between the control group and the vehicle control group, but the fluorescence intensity was significant⁃ ly lower in ZHX3 gene silence group than that of two control groups. (4) There were no significant differences in expressions of smad3, smad4 and RUNX2 betweem control group and the vehicle control group, but they were significantly higher than those of ZHX3 silence group（P < 0.05）. Conclusion ZHX3 gene silence can delay vitro osteogenesis of BMSCs, which may play a role by the down-regulated expression levels of smad3, smad4 and RUNX2.

Key words: DNA-binding proteins, zinc fingers, genes, homeobox, transcription factors, geen silencing, mesenchymal stem cells, smad3, smad4, RUNX2, zinc fingers and homeoboxes 3