Tianjin Medical Journal

Tianjin Med J ›› 2016, Vol. 44 ›› Issue (4): 449-452.doi: 10.11958/20150146

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The effects of combination of paeonol and PNS on TGF-β/Smad pathway in ventricular remodeling after myocardial infarction in rats

NIE Dan1 , SUN Hongdan1 , SHI Zhaoping2 , ZHOU Xiaohui 1△   

  1. 1 Key Laboratory of Study and Exploiture for New Drugs of Traditional Chinese Medicine (TCM)， Institute of TCM Medical of Chengde Medical College， Chengde 067000， China； 2 Chengde Traditional Chinese Medicine Hospital
  • Received:2015-09-06 Revised:2015-11-10 Published:2016-04-15 Online:2016-05-20
  • Contact: △Corresponding Author E-mail： zxh5055@sina.com E-mail:18729542245@163.com

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Abstract: Abstract:Objective To study the effect of paeonol (PAE) and PNS on the expression of transforming growth factor (TGF)- beta 1/ Smad2/3 pathway in rats with acute myocardial infarction (AMI), and the possible molecular mechanism thereof. Methods Model of AMI was made using left anterior descending coronary branch ligation. According to the inter⁃ vention methods rats were divided into model group, PAE group (8 mg·kg- 1 ), PNS group (40 mg·kg- 1 ), PAE (4 mg·kg- 1 ) + PNS (20 mg·kg-1 ) low dose group, PAE (8 mg·kg-1 ) + PNS (40 mg·kg-1 ) high dose group and captopril positive control group (10 mg·kg-1 ). Rats without ligation were used as Sham operation group. Left ventricular systolic blood pressure (LVSP), left ventricular diastolic pressure (LVEDP) and the maximum rise and fall rate (/dtmax DP) were detected after 28-day treat⁃ ment. HE staining was used to observe changes of myocardial tissue. The protein expression levels of TGF-β1 and Smad2/3 were detected by Western blot assay. Results There were significant differences in parameters used for detecting treatment group and model group, formula group and single drug group, formula high dose group and formula low dose group (P < 0.01). The model group showed pathological changes. All treatment groups showed different degrees of pathological improve⁃ ment. There was the most significant improvement in formulae group and captopril group. Compared with the model group, TGF-β1 and Smad2/3 protein expressions were decreased in treatment group. The expression levels of TGF-β1 and Smad2/3 were significantly decreased in formula group than those of PAE group and PNS group, and lower levels in formula high dose group than those of formula low dose group (P < 0.05). Conclusion Paeonol and PNS can inhibit the expressions of TGF-β/ Smad 2/3 protein in rats with AMI, by blocking TGF-β / Smad pathway.

Key words: myocardial infarction, disease models, animal, ventricular remodeling, transforming growth factor beta, drug therapy, combination, Smad2/3, compound of Paeonol and PNS