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15 November 2010, Volume 38 Issue 2 Previous Issue    Next Issue
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Construction of a multi-copy secretory exspresstion vector and hirudin expression in Pichia pastoris 2010, (2):  0-0 .  Abstract ( 832 )   Abstract Objective: To construct suitable vectors for the secretory expression of hirudin in Pichia pastoris. Methods: The α-facor- Hirudin gene was amplified from pPIC9- Hirudin by PCR and subcloned into PAO815. and then construct multicopy recombinant plasmid pAO815-(α-Hirudin)n .The recombinant was transformed into P. pastoris strain GS115 for induction expression and then the activity of secreted products were identified. Result: A new multicopy vector pAO815-(α-Hirudin)n was successfully constructed and was capable of secreting recombinant hirudin efficiently, confirmed respectively by PCR, SDS-PAGE. The products possess the activity of thrombin inhibitor. Conclusion: This result offers efficient P. pastoris strains for mass production of biologically active hirudin. Related Articles | Metrics

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Report of children influenza A (H1N1) cases in Tianjin 2010, (2):  0-0 .  Abstract ( 1262 )   Objective To summarize the clinical characteristics of seven children sporadic influenza A (H1N1) cases broken out in Tianjin in order to provide clinical experience and prognosis for the diagnosis and prevention of this disease. Methods The clinical data and protective measures of seven children influenza A (H1N1) inpatients confirmed by Tianjin Haihe Hospital from June 9, 2009 to September 21, 2009 were retrospectively analyzed ,and related literature was reviewed. Results There are six imported cases and one second-generation case. Fever and cough were the main symptom,Five were hyperpyrexia,three were with expectoration. Other symptoms include anergy, dizziness,headache,nasal discharge,sneeze and pharyngalgia. two children with leukocytosis decreases, two children complicating infection with leukocytosis increase,others are normal. five with normal chest X-ray, one with texture disorder, and one with bilateral multiple infiltrating image. Influenza A(H1 N1)virus confirmed was detected in the throat swab samples taken within 2 days from the seven children by real?time PCR and sequencing comparison,and were tested negative after eight to nine days in the course of this disease. Six patients were accepted Oseltamivir treatment for five days, one was only treated with anti-virus mixture.The average hospital stay was eight days.When they were discharged from hospital, all the symptoms have disappeared All the patients’ families, doctors and nurses were not infected in the case of non-directly exposed to the disease. Conclusion The seven children patients mainly showed symptoms of fever and cough,The average course is 9.1 days, and their prognosis were good. Therefore, influenza A (H1N1) is preventable and curable. Related Articles | Metrics

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Regulation of MCP-1 in osteoblasts by PTHrP in breast cancer cells 2010, (2):  0-0 .  Abstract ( 794 )   Abstract Objective: To identify regulation of MCP-1 in osteoblasts by PTHrP in breast cancer cells. Method: Osteoblast-like UMR106 cells were treated with 10-8 M PTHrP (1–34) for different time intervals and expression of MCP-1 was studied by using semiquantitative RT-PCR. Recombinant shRNA plsmid PTH1R/pRNAT was constructed, transfected into UMR106 cells, and stable PTH1R/UMR106 cells were established in which PTH1R expression was knocked-down. Thereafter, the conditioned medium (CM) of breast cancer MDA-MB231 was added to UMR-106 and PTH1R/UMR106 cultures respectively. The mRNA of MCP-1 treated by PTHrP and CM of breast cancer was examined by RT-PCR. Result: PTHrP induced expression of MCP-1 in UMR106 cells and the induction began at 3 h and reached maximum at 6 h. Treatment of UMR106 cells with CM of MDA-MB231 also significantly induced mRNA of MCP-1, and this effect was blocked by the knockdown of PTH1R in UMR106 cells. Conclusion: Breast cancers upregulated MCP-1 level in osteoblasts through expression of PTHrP. Related Articles | Metrics

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Aspirin resistance and off-pump coronary artery bypass grafting surgery Gao Fei, 2010, (2):  0-0 .  Abstract ( 1170 )   Objective:To assess aspirin efficacy in the early postoperative period. Methods:Sixty patients undergoing OPCAB surgery were enrolled in the study. Functional and biochemical responses to aspirin were evaluated by arachidonic acid (ARA)-induced platelet aggregation and urine 11-DH-TXB2 metabolite excretion. Previous aspirin treatment was terminated 5-7days before OPCAB and continued at the day with food intake after surgery. Samples were collected before and on days 1, 4 and 10 after aspirin intake(100mg/d). Results:Patients with aspirin resistance on days 1,4and 10 were 17,9 and 2, separately, and the level of platelet aggregation was stable on the 4th day after aspirin intake. Conclusion:Aspirin did not sufficiently inhibit platelet aggregation early after OPCAB. It is important to keep potential anti-coagulation therapy in the early postoperative period. Related Articles | Metrics