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15 December 2010, Volume 38 Issue 12 Previous Issue    Next Issue
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论著
实验研究
短篇论著
临床论丛
短篇与个案


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论著

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Study on the expression of Artemin in Esophageal carcinoma 2010, 38 (12):  1025-1027 .  Abstract ( 1140 )   PDF (495KB) ( 1873 )   Objective To investigate the expression of artemin in esophageal carcinoma. Method By Immunohistochemistry and real-time PCR,used to detect the expression of artemin in 126 esophageal carcinoma and carcinoma adjacent tissue, 21 normal esophageal tissue.RNA interference technology was used to suppress the expression of artemin protein in TE11 esophageal carcinoma cell line,we observed the effect of apoptosis . Results The positive rate of artemin in esophageal carcinoma was significantly higher than those in carcinoma adjacent tissue and normal tissue. In addition ,the small interferon RNA expression vector can effectively inhibit the expression of artemin in TE11 cell line,and then promoted the apoptosis of TE11 cell line. Conclusion The expression of artemin was relate to the biological behavior of esophageal carcinoma. Related Articles | Metrics

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Relationship between TLR3 expression in placenta and intrauterine infection of hepatitis B virus 2010, 38 (12):  1028-1031 .  Abstract ( 1489 )   PDF (761KB) ( 1948 )   Abstract Objective：To study on the distribution of toll-like receptor 3 (TLR3) in human placenta and their role in the HBV intrauterine infection. Methods：Twenty two full-term placental tissues of HBsAg carriers and 6 ones of HBsAg sero-negative pregnant women were collected. The HBsAg and TLR3 in the placental tissues were investigated by StreptAnidin-Biotin peroxidase Complex (SABC) immunohistochemical staining. Neonatal HBsAg and HBeAg was tested by Chemiluminescence. HBsAg in maternal sera were detected by ELISA. Results：The HBsAg positive rate in the full-time placental tissues from HBsAg carrier pregnant women was 77.27% (17/22). The positive signals of HBsAg could locate in the cytoplasms of decidual cells, trophoblastic cells, villous meseachymal cells and villous capillary endothelial cells. The positive signals of TLR3 major locate in the cytoplasm of trophoblastic cells and villous meseachymal cells in the HBsAg-positive placental tissues. TLR3 could not be found in the placenta of HBsAg sero-negative pregnant women. Conclusion: Preliminary considering, there may be only weak response of TLR3 to HBV stimulation. Related Articles | Metrics

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Expression of TRβ1 gene mRNA in breast cancer and its clinical significance 2010, 38 (12):  1032-1034 .  Abstract ( 1631 )   PDF (801KB) ( 1922 )   Abstract Objective:. To study the expressions of TRβ1 mRNA and clinical significance in breast cancer.Methods：The expressions of TRβ1mRNA in 40 cases of breast cancer and 10 cases of normal tissue were detected and analysed by using reverse-transcription PCR (RT-PCR). Results：The TRβ1 gene was expressed in all normal breast tissue. By contrast, reduced expression of TRβ1was found in 34 of the 40 breast cancer tissue samples (85%). The expression levels of TRβ1 were significantly lower in cancer group than those of normal group (P < 0.05).The expressions of TRβ1mRNA were significantly related with the depth of invasion, lymph-node metastasis and TNM stage(P < 0.05). but not related with age (P ＞ 0.05)，. Conclusion： TRβ1 play an important role in invasion and metastasis of the breast cancer. Detection of TRβ1 may be a new target for cancer diagnosis to assess the severity of breast cancer. Related Articles | Metrics

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Expressions of PTEN and p38MAPKs in Benign and Malignant Prostatic Lesions 2010, 38 (12):  1035-1037 .  Abstract ( 1074 )   PDF (844KB) ( 1931 )   Objective: To study the different expression and clinical significance of PTEN and p38MAPK in benign and malignant prostatic lesions. Methods: SABC immunohistochemical analysis was used to determine the expression levels of PTEN and p38 in 20 cases of benign prostatic hyperplasia(BPH),20 cases of prostatic intraepithelial neoplasia(PIN) and 46 cases of prostatic carcinoma (PC). Results: Expression of PTEN was the highest in BPH and significantly higher in PIN compared to PC (P<0.01). Expression of p38 was the highest in PC and significantly higher in PIN compared to BPH(P<0.05).PTEN protein expression in prostatic carcinoma was negatively correlated with tumor stage, and histological grade(r=-0.352,-0.653,P<0.05)；while p38 expression was positively correlated with the above features(r=0.298,0.596,P<0.05).There was a negative correlation between PTEN and p38 expression(r=-0.602,P<0.01)．Conclusions: Low PTEN protein expression and elevated activation of p38 are involved in the malignant transformation of prostatic epithelium and may play a great role in the genesis and development of prostatic tumor. Related Articles | Metrics

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Apoptosis-Inducing effects of ribavirin on K562 leukemia cells 2010, 38 (12):  1038-1040 .  Abstract ( 1394 )   PDF (875KB) ( 1895 )   Objective To determine the apoptosis-Inducing effects of ribavirin on K562 leukemia cells. Methods Apoptosis-Inducing effects of a neurotransmitter ribavirin were evaluated by proliferation, morphology and detection of apoptosis apex through flow cytometry. We evaluated the effects of ribavirin on K562 cells proliferation by cellular enumeration inMTT assay and clony formation assay, the morphology by Wright-Gimesa(W-G) staining, Results ribavirin inhibited the proliferation of K562 cells, induced the apoptosis apex, and showed apoptosis morphology after drug treatment. Conclusion Ribavirin possessed apoptosis-inducing effects on K562 cells. Related Articles | Metrics

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The Clinical Research of Super Early Stage Transsyvian Transinsular Approach on Hypertensive- Cerebral Hemorrhage in Basal Ganglia Regions 2010, 38 (12):  1041-1043 .  Abstract ( 1903 )   PDF (959KB) ( 2190 )   Abstract Objective：To explore a new surgical method to treat hypertensive basal ganglia hemorrhage (HBGH) and its curative effect．Methods：A total of 21 patients with hypertensive cerebral hemorrhage in the fundus node at a super early stage (≤7h) were enrolled in this study．Microsurgery trough the lateral fissure and insula via pterional keyhole approach was employed to treat the patients．The CT reexamination within 2 days post-operation among all patients and they were evaluated by activities of daily living (ADL) after the operation 3-12 months．Results：The CT showed that hematoma clearance rate>90% was achieved in 18 cases，80~90% in 3 cases．All but one of the patients survived after the operation．The patients were evaluated by activities of daily living (ADL) after the operation． ADLⅠ was found in 4 cases，ADL Ⅱin 12 cases，ADL Ⅲ in 3 cases，ADL Ⅳ in 1 case and ADL Ⅴin 0 case． One case died． Conclusion：Super Early Stage Transsylvian Transinsular Approach on Hypertensive- Cerebral Hemorrhage in Basal Ganglia Regions had the advantages of short operating time，short pathway，minimal invasion and better patient recovery．It may improve in combination with comprehensive therapy． Related Articles | Metrics

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Dynamic viewing of Streptococcus mutans LuxS mutant in Biofilm formation 2010, 38 (12):  1044-1046 .  Abstract ( 1581 )   PDF (1013KB) ( 1953 )   [Abstract] Objective To study the difference of biofilm formation at different times between Streptococcus mutans Ingbritt C international standards strain and LuxS mutant strain by dynamic viewing of converted fluorescence microscope. Methods The biofilm models of both Streptococcus mutans standards strain and LuxS mutant strain were built on the slides carriers in 6,18,24,48h respectively. The biofilms of the two strains and the relative mobility between the bacteria were observed using converted fluorescence microscope after dyeing by means of acridine orange(AO). Results The biofilms in the microtiter plates could be observed dynamically by converted fluorescence microscope, There are variety in morphology of the two stains; the biofilms began to form with the bacteria gathering densely at 6h.Those of 18h and 24h were the best formation, they can adhere to slides carrier very firmly and can not be washed out; at the time of 48h,the adhesive capacity of the standards strain was lower than LuxS mutant strain, the whole biofilm of the standards strain was prone to slide. Conclusion The biofilms of Streptococcus mutans standard s strain and LuxS mutant strain were three-dimensioned and variety in morphology, in addition, at different point-in-times, they displayed a marked difference in the form of biofilm , reciprocity of bacteria and adhesive power. Related Articles | Metrics

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The function of helper T lymphocytes in ankylosing spondylitis 2010, 38 (12):  1047-1049 .  Abstract ( 1304 )   PDF (1140KB) ( 2120 )   [Abstract] Objective To study the role of the imbalance of Th1/Th2 and Th1/Th2 cytokines in the pathogenesis of AS.Methods 30 subjects patients with AS and and 20 subjects normal controls had been chosen. Enzyme linked immunosorbent assay (ELISA) was used to measure the levels of TNF-α and IL-10 in the serum and synovial fluid. Three-color flow cytometric analysis was employed for surface antigens (CD3, CD4) of Th cells and intracellular cytokines (IFN-γ/IL-4) of Th1/Th2 in the peripheral blood of patients with AS and normal controls.Result The TNF-α and IL-10 serum levels in patients with AS were all significantly higher than in normal controls(P<0.05); The synovial fluid level of TNF-α was significantly higher than the serum level (P<0.05), The synovial fluid level of IL-10 was not significantly different from the serum level (P>0.05); The percentage of Th1 and Th2 cells in the peripheral blood of patients with AS were not significantly different from normal controls (P>0.05),The percentage of Th1 cells in the peripheral blood of patients with AS was significantly higher than normal controls, The rate of Th1/Th2 was significantly higher than in normal controls (P<0.05)；The serumlevel of TNF-α correlated significiently with ESR, CRP. There wasn’t significant correlation between the serum level of IL-10、the percentage of Th1 cells (or Th2 cells) 、 the rate of Th1/Th2 and ESR, CRP (P>0.05). Conclusion There is an imbalance in Th1/Th2 in the peripheral blood of patients with AS, and towards Th1. Related Articles | Metrics

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The Expression and Significance of Aromatase Cytochrome P450 in Endometrium with Endometriosis 2010, 38 (12):  1050-1052 .  Abstract ( 1194 )   PDF (1192KB) ( 2111 )   Objective: To investigate the expression and clinical significance of P450arom in patients endometriosis. Methods: Immunohistochemistry was used to detect the expression of P450arom in the endometrium. All data were statistically analyzed. Results: The the expression level of P450arom was significantly higher in patients with endometriosis than the control group( P =0.0006 ).Its sensibility was 80.95%, the specifility was 82.50% in the diagnosis of endometriosis. Conclusion: The higher-expression of P450arom in endometrium was related to the development of endometriosis. P450arom may help evaluate endometriosic development. Related Articles | Metrics

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Clinical study of acute myocardia infarction with paroxysmal atrial fibrillation 2010, 38 (12):  1053-1055 .  Abstract ( 1179 )   PDF (1194KB) ( 1930 )   （Abstract）Objective: To observe the clinical causes on the recurrences of atrial fibrillation (AF) of patients with acute myocardial infarction(AMI) and paroxysmal atrial fibrillation (AF). Methods: 120 cases of patients with AMI and AF are divided, on whether or not recurrence of AF happened in the one-year follow-up, into two groups: the recurrence group and the normal sinus rhythm (NSR) group. Such data as general information, medical history and treatment of patients of the two groups are compared. At the end of the one-year follow-up, examinations of the patients are conducted on C-reactive protein (CRP), low-density lipoprotein (LDL), left atrial diameter (LAD) and left ventricular ejection fraction (LVEF), and their cardiac function are examined and classified. Results: The averaged age of recurrence group were apparently higher than the NSR group (P<0.01). There were more cases with high blood pressure, anterior myocardial infarction (P<0.01or P<0.05) and lower cardiac function (P<0.01), reveal a lower LVEF (P<0.01) and a larger LAD (P<0.01). On the other hand, the NSR group have lower CRP and LDL (P<0.01) level. Observations on the different treatments showed that patients with successful intravenous thrombolysis and those with treatment of statins have significantly few cases of AF recurrence compared with patients without the application of intravenous thrombolysis and failure to reperfusion. Conclusion: Precipitating factors for the recurrence of AF include senior age, high blood pressure, anterior wall infarction, left atrial enlargement and deterioration of cardiac function. Therefore, early revascularization and treatment with statins are effective measures against the recurrence of AF. Related Articles | Metrics

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The Research On T lymphocyte Function In Type 2 Diabetes Mellitus Patients With Black Patches On Pre-tibia 2010, 38 (12):  1056-1058 .  Abstract ( 1557 )   PDF (1203KB) ( 2192 )   【abstract】Objective To investigate T lymphocyte function and prevalence of microangiopathy in Type 2 diabetes mellitus patients with black patches on pre-tibia. Methods Percentage of CD3+ to total lymphocytes was measured through flow cytometry, so were the percentages of CD4+ and CD8+ to T lymphocytes. Results (1) There was no significance between black-patch group and non-black-patch group in the percentage of CD3+ to total lymphocytes（P＞0.05）; The percentage of CD8+ to T lymphocytes in the black-patch group was significantly lower than that in the non-black-patch group （P＜0.01）; Both of the the percentage of CD4+ and the ratio of CD4+/CD8+ in the black-patch group were significantly higher than those in the non-black-patche group（P＜0.01）.(2) Incidence of chronic complications, especially microangiopathy, in the black-patche group was significantly higher than that in the non-black-patch group （P＜0.05）Conclusion Black patches on pre-tibia could be a dermatic sign of immune disorder and microangiopathy in type 2 diabetes mellitus. Black patches and other microangiopathy in type 2 diabetes mellitus could be related to disorder of cyto-immune function. Related Articles | Metrics

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Modification of Heart and Its Effect Factors in Patients with Type2 Diabetes Mellitus Accompanying Hypertension 2010, 38 (12):  1059-1061 .  Abstract ( 1822 )   PDF (1219KB) ( 1887 )   Abstract： objective : To Explore the effect factors of left ventricular hypertrophy and left ventricular diastolic dysfunction in patients with type2 diabetes mellitus accompanying hypertension. Methods: one hundred and fifty-two patients with type2 diabetes mellitus accompanying hypertension (A group), seventy-nine essential hypertension patients ( B group) and thirty-eight healthy individual (C group) were submitted to 24-h Ambulatory Blood Pressure Monitoring (ABPM) and their echocardiography with Doppler、HbA1c and BMI were tested. some index changes were compared among groups including BMI 、HbA1c 、age 、sex 、 diurnal systolic blood pressure (DSBP)、 nocturnal systolic blood pressure(NSBP) 、LVMI 、LVH 、IVRT and E/A rate etc. Results: 1、HbA1c in A group is significantly higher than that in other two groups; BMI、sex and age did not differ among A. B. C groups. 2、Both NSBP and NDBP in A group and B group were higher than those in C group, NSBP in A group is higher than that in B group. 3、LVMI in A group exceed that in B group and C group ,the incidence of LVH in A group beyond that in B group and C group(20.4% vs 12.7% vs 2.6%, P<0.05,respectly),IVRT both A group and B group is higher than that in C group, E/A rate in A group is lower than that both B group and C group. Conclusion : LVMI increased and incidence of LVH risen in patients with hypertension and accompanying left ventricular diastolic dysfunction, moreover, those modification were worsen by diabetes mellitus, hyperglycemia and NSBP levels decrease deficiency should be responsible for them. Related Articles | Metrics

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The relationship of Plasma omentin level And Metabolic syndrome 2010, 38 (12):  1062-1064 .  Abstract ( 1482 )   PDF (1223KB) ( 2085 )   Abstract Objective: We explored the relationships between 2 diabetes mellitus with Metabolic syndrome and plasma omentin level. Methods: Fasting plasma omentin level of T2DM (forty nine patients with Metabolic syndrome ,forty six patients without Metabolic syndrome) were assayed by enzyme-linked immunosorbent asssy(ELISA), and thirty six controls, The relationship of plasma omentin level with blood glucose,blood lipids, FINs, WHR,BMI,HOMA-IR,SBP,DBP were also analyzed. Results：The plasma omentin level was significantly lower than controls（P<0.01）. Plasma omentin level in T2DM with MS was significantly lower than without MS（P<0.01）.correlation analysis showed that the plasma omentin level was negatively in correlation withWC,TG,HOMA-IR ,Fins,2hPG,FBG,SBP,DBP（r=-0.465，-0.696，-0.648，-0.691，-0.635，-0.380，-0.663，-0.522 P<0.01）, and was positively in correlation with HDL（r= 0.279 P<0.01）.Stepwise regression indicated that BMI,2hPG and WC were independent correlation fators that influenced the level of omentin . Conclusion :Omentin was associated with obesity and insulin resistance in patients with MS. It contributes to the process of MS. Related Articles | Metrics

实验研究

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The effects of insulin on the expression of VEGFR in RF/6A cells 2010, 38 (12):  1065-1067 .  Abstract ( 1094 )   PDF (1230KB) ( 1880 )   Objective: To study the effect of insulin on angiogenesis in RF/6A retinal vascular endothelial cells and elucidate the underlying mechanism. Methods : RF/6A cells were treated with 01nmol/L、 1nmol/L and 100 nmol/L of insulin respectively and MTS, cells migration experiment and lumen formation experiment were used to test cell proliferation, migration and lumen formation respectively,.RT-PCR and Western bot were used to measure the expression of vascular endothelial growth factor receptors VEGFR and phosphorylated receptors . Results : Insulin promoted RF /6A cell proliferation, migration and lumen formation (all P<0.01). Insulin also increased the both the expression of VEGFR2 mRNA and its phosphorylation (P< 0.01or P<0.05). There was no significant difference in the expression of VEGFR1 mRNA among the groups (P> 0.05). Conclusion Insulin might promote RF/6A angiogenesis via a VEGFR2 mediated mechanism that does not involve VEGFR1. Related Articles | Metrics

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Immunoregulation Effects of Total Flavones of Epimedium on Immunosuppression Mice 2010, 38 (12):  1068-1071 .  Abstract ( 1438 )   PDF (1273KB) ( 2077 )   Abstract Objective:To investigate the immune regulatory function of total flavones of Epimedium on immunosuppression mice. Methods:The immunosuppression model was established by using cyclophosphamide(CY). The body weight and spleen weight were analysed. The absolute number of total splenocytes and bone marrow cells were determined by haemacytometer.The absolute number of CD4+T cells ,CD8+ T cells,B cells and NK cells in spleen was detected by flow cytometer. Results:Total Flavones of Epimedium significantly antagonist the loss in immune organ and body weight induced by cyclophosphamide, markedly restore the number of total splenocytes and bone marrow cells , improved the number of CD4+T cells ,CD8+ T cells,B+ cells and NK cells in spleen . Conclusion:Total Flavones of Epimedium could improve the immune function in immunodepressed mice induced by CY. Related Articles | Metrics

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Expression of P53 and VEGF in serum and xenograft of SKOV3 tumor-bearing nude mice model of adverse psychological stress and their significances 2010, 38 (12):  1072-1075 .  Abstract ( 1401 )   PDF (1320KB) ( 2006 )   purpose： To investigate the effects of adverse psychological stress on p53 and VEGF protein levels in serum and xenograft of SKOV3 tumor-bearing nude mice. Methods: The adverse psychological stress model of nude mice bearing ovarian cancer were established, the levels of P53, VEGF in serum were detected by ELISA in normal control, simple stress, simple tumor and tumor-bearing stress group, immunohistochemistry and Western Blot were performed to detect the expression of P53, VEGF protein for xenografts in the tumor group and tumor-bearing stress group. Results : The levels of P53 and VEGF in serum of stress groups were higher than those in corresponding group that not subjected to stress, the differences were significant (P <0.05). Western Blot showed that the average density value ratio of P53 and VEGF in tumor-bearing stress group were 2.163 ± 0.072 and 2.523 ± 0.088, respectively, while those in simple tumor group were 1.116 ± 0.068 and 1.477 ± 0.083, respectively, the differences were significant (P <0.01).The results of immunohistochemistry showed that P53 protein in tumor-bearing stress group (n = 32) and tumor group (n = 24) positive expression rates were 87.5% and 62.5%, while VEGF protein were 93.8% and 70.8%, the differences were significant (P<0.05). Conclusion: adverse psychological stress may accelerate the progress of SKOV3 tumor, the expression of P53 and VEGF may be concerned in the mechanism; P53 and VEGF are expected to become the molecular targets for psycho-oncology study. Related Articles | Metrics

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Effects of Tirofiban on Inflammation Factors in Pigs with Myocardial Infarction Reperfusion 2010, 38 (12):  1076-1079 .  Abstract ( 1299 )   PDF (1774KB) ( 1939 )   Objective：To investigate the effect of tirofiban on inflammation f actors in pigs with myocardial infarction reperfusion . Methods：19 Chinese mini pigs were randomized into control group （n=10）and tirofiban-treating group（n=9）. Acute myocardial infarction was induced by method of PTCA. All the successful models accepted DE-MSCT examinations at 1 hour, 24 hours, 48 hours, and 72 hours after reperfusion. All pigs was taken blood at seven different points: 30min before AMI, 30min after AMI, 10min, 2h, 24h, 48h, 72h after reperfusion. The serum levels of IL-6, IL-10, HIF-1αwere measured by enzyme linked immunosorbent assay(ELISA). Result ：（1）The infarct myocardium volume percent was significantly reduced in tirofiban-treating group compared with control group at 24h, 48 and 72h after reperfusion; while the no-reflow volume percent was significantly reduced in tirofiban-treating group compared with control group from 1h to 72h after reperfusion. （2）In tirofiban-treating group, the levels of serum IL-6 were significantly lower than those in control group(p<0.05)，while the levels of serum IL-10 were higher than those in control group(p<0.05).（3） There were no statistical differences between the two groups(p>0.05).Conclusion：Inflammation reaction occurs in ischemic myocardium after AMI and reperfusion ; tirofiban is effective in anti-inflammation, reducing the progress of myocardial necrosis. Related Articles | Metrics

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Analysis of the expression of Hes1 in adult mouse hippocampus after traumatic brain injury 2010, 38 (12):  1080-1082 .  Abstract ( 1083 )   PDF (1338KB) ( 2010 )   Objective:To observe and analyze the changes of the expression level of Hes1 in adult mouse hippocampus after traumatic brain injury(TBI). Methods:The C57BL/6 mice were randomly divided into sham operation group(NC,n=33) and traumatic brain injury group(TBI,n=33). Here,we established the experimental TBI model by means of lateral fluid percussion injury(FPI).Then，mice were killed by cervical dislocation 6h,1d,3d and 7d after operation. The expression of Hes1 gene in adult mouse hippocampus was detected by real time PCR and Western blot ,fluorescence immunohistochemistry staining technique at mRNA and protein level.GAPDH is as the internal reference in real time PCR and Western blot. Results:Hes1 was expressed in all hippocampus of TBI group mice. Compared with the NC group（1.03±0.19，1.02±0.14，1.04±0.16，1.06±0.20）, the expression of Hes1/GAPDH mRNA in traumatic lateral hippocampus 6 h , 1d, 3d and 7d after TBI were respectively 6h(1.37±0.14),1d(0.65±0.17),3d(0.46±0.14)和7d(0.82±0.10); the expression of Hes1 protein in traumatic lateral hippocampus on 3d after TBI was (1.25±0.21) contrast to NC groups’ (0.39±0.17).The differences is statistically significant(P<0.05). Fluorescence immunohistochemistry staining shows that Hes1 protein expression was wide in the hippocampus cells of NC group mice, compared with which, TBI group mice’ 3 days after injury was significantly decreased. Conclusion: Hes1 maybe involved in the neurogenesis process and plays an important role in subsequent repair. Related Articles | Metrics

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Thalidomide influence the growth and angiogenesis patterns of melanoma 2010, 38 (12):  1083-1086 .  Abstract ( 1859 )   PDF (1905KB) ( 1916 )   OBJECTIVE: The aim of this research was to examine the influence of thalidomide on the growth and angiogenesis of melanoma. These results would be hoped to elicit a novel approach to the clinical treatment of melanoma. METHODS: Thirty-five C57 mice of the melanoma transplanted model was lavage by thalidomide and 0.5% CMC. The vasculogenic mimicry, mosaic vessel, endothelial dependent vessel and necrosis ratio of tumor were measured by H&E staining. The expression level of MMP-2, MMP-9, VEGF, NF-κB and PCNA were examined by immunohistochemistry. RESULTS: The tumor volume of experiment group was statistically significantly decreased compared with the control group（P<0.05）. The vasculogenic mimicry, mosaic vessel and endothelial dependent vessel count in experiment group were statistically significantly decreased compared with the control group（P<0.05）. The necrosis ratio of tumor was increased compared with the control group（P<0.05）. The expression level of MMP-2, MMP-9, VEGF, NF-κB and PCNA of experiment group tumor tissues were statistically significantly decreased compared with the control group（P<0.05）. CONCLUSIONS: Thalidomide could inhibit the vasculogenic mimicry, mosaic vessel and endothelial dependent vessel information, induce necrosis to inhibit tumor growth. Related Articles | Metrics

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The intervention and mechanism of PNS on rat pulmonary fibrosis 2010, 38 (12):  1087-1089 .  Abstract ( 1389 )   PDF (1400KB) ( 2083 )   【Abstract】 Objective To observe The inhibitory effect of PNS on rat pulmonary fibrosis and to discuss the mechanism of this action.Method Divide 80 only rats randomly into the model group,PNS rental ,methylprednisolone group and the combined treatment group. Adopting bleomycin prepare pulmonary fibrosis in rats, 24 hours later ,give them medication every day,then kill those rats respectively at the 7th、14th、21th、28th day and observe its pathological changes by detecting the expression levels of ICAM-1, TGF-β1, MMP-9 in lung tissue.Results The PNS rental，methylprednisolone group and the combined treatment groups are no serious Alveolitis and pulmonary fibrosis.The expression levels of ICAM-1, TGF-B1, MMP-9 of PNS rental, methylprednisolone group, and the combined treatment group are significantly decrease compared with the model group（p<0.01）. The combined treatment group’s expression levels of TGF-β1 are lower than the two single medicine treatment group(p<0.05).Conclusion the PNS can effectly restrain pulmonary fibrosis process induced by bleomycin,and using corticosteroids together can reduce hormone dosage and side effects, while enhancing the treatment effect. Related Articles | Metrics

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The effect of different concentrations of alcoholon the expression of TGFβ-1 and bFGF in rats’lung tissue 2010, 38 (12):  1090-1092 .  Abstract ( 1735 )   PDF (1346KB) ( 1937 )   Objective:To observe the effects of different concentrations of alcohol intake and the expression of TGF-β1 and bFGF on pulmonary in rats, and to explore the relationship between pulmonary fibrosis and possible mechanisms. Methods: Clean Wistar male rats (n=32) were randomly divided into control group (C, n=8), free drinking water; alcohol group (A, n=24), according to different concentrations were divided into 3% alcohol group (A3, n=8), 10% alcohol group(A10,n=8), 20% alcohol group(A20,n=8). A certain concentration of liquor was drunk freely by the rats of alcohol group, with no other liquid feed. Animals of each group were put to death 16 weeks after anesthesia. The transforming growth factor β1 and the basic fibroblast Growth factor in the rat lung tissue were measured through immunohistochemistry. By Masson staining, the interstitial lung collagen deposition in the case was observed. Results: Immunohistochemical results showed: (1) the positive cells of TGF-β1: The difference between control and alcohol group is significant (P < 0.05). To compare the C with the A20, 10 groups, the differences are statistically significant (P < 0.05). But to compare with the A3 group, the difference is not significant (P > 0.05). (2)The positive cells of bFGF: The differences between control and alcohol group is significant (P < 0.05), and the differences between the alcohol group of different concentration are also significant (P < 0.05). Masson results showed the deposition of collagen in the alveolar interval increased more significantly in alcohol group than the control group, especially in the A10 and A20. Conclusions: Our study proves that the expression of TGF-β1 and bFGF increase in the alveolar type Ⅱ epithelial cells and macrophages as the increase in the concentration of alcohol. A certain concentration of alcohol would cause lung collagen deposition. Our study also suggests that alcohol may be one of the reasons for leading to pulmonary fibrosis. Related Articles | Metrics

短篇论著

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Logistic Regression Analysis Findings of the Urinary Calculus Caused by Melamine - Contaminated Milk Powder 2010, 38 (12):  1093-1094 .  Abstract ( 1681 )   PDF (946KB) ( 1953 )   【Abstract】Objective Through epidemiological Investigation of the urinary calculus caused by melamine-contaminated milk powder, to explore the risk factors of the disease and provide a reliable basis for preventing the disease’s incidence.Methods A 1:2 matched case control study was conducted to investigate 26 cases and 52 the control, the cases were urinary calculus result from melamine-contaminated milk powder, the control has’t have urinary calculus caused by melamine-contaminated milk powder, matching factor with sex, and the different of age less than or equal to one year. The data were analyzed for Single and Multiple factor conditional logistic regression. Results The results of OR : the history of urinary lithiasis with the first degree relative(12.321) , infancy fed with melamine-contaminated milk powder (6.792), take calcium (3.454), drink water(0.359).Conclusion The results of multiple regression model showed that the risk factors of this disease were the history of urinary lithiasis with the first degree relative, infancy fed with melamine - contaminated milk powder and take calcium,the protective factor was to drink water. Related Articles | Metrics

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Analysis of Clinical Features in 41 Cases with Late-Onset Systemic Lupus Erythematosus WANG Ling1, 2 2010, 38 (12):  1094-1095 .  Abstract ( 1411 )   PDF (968KB) ( 2035 )   Objective：To examine the clinical features of patients with late oneset systemic lupus erythematosus (LOSLE). Methods：41 cases with LOSLE between 2007 and 2009 were reviewed and analyzed ，and compaired with 81 patients who developed SLE below 50 by matching their clinical manifestations, laboratory data and complications. Results：Compaired with the control group, the LOSLE group had a lower female to male ratio(p<0.01); but the incidences of anorexia、myalgia、anergy、weight descent、pulmonary involvement、secondary sjogren syndrome and positive RF、positive anti-SSA were significant higher (p<0.05); the incidences of lupus nephritis、photo sensitivity、anemia、leukopenia、platelet cytopenia、malar rash、positive anti-dsDNA and low complement were significant lower (p<0.05). Conclusion：compared with young and middle-aged SLE patients, the clinical manifestations of LOSLE patients’ are atypical, SLEDAI scores are lower, but complications more than the former. Related Articles | Metrics

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The influences of arch width and smile esthetics by extraction and nonextraction 2 2010, 38 (12):  1096-1097 .  Abstract ( 1561 )   PDF (1003KB) ( 2376 )   ABSTRACT Objective:To compare the changes of arch width as well as smile esthetics in patients treated by extraction and nonextraction procedures. Methods: Dental casts of 30 patients treated with extraction and 30 patients without extraction of four first premolars were randomly selected. Arch widths were measured from the cusp tips of the canines, premolars, and molars. Posttreatment arch widths were also measured in the midline at a constant arch depth from the most labial surfaces of the incisors. Standardized frontal photographs of the face taken during smiling of extraction and nonextraction treated subjects were evaluated. Twenty orthodontic doctors judged the esthetics of the smiles. Results: Intercanine width increased less than one mm in both groups, and there was no difference between the two groups. The interpremolar and intermolar distance in both arches decreased significantly in theextraction sample, whereas the interpret molar and intermolar widths increased significantly in the nonextraction sample. When arch widths of both groups were measured from the most labial surfaces of the teeth at a constant depth, the average arch width of both arches was significantly wider in the extraction sample. The mean esthetic score and the number of teeth displayed during a smile did not differ between the groups. Conclusion: The results indicate that arch width is not decreased at a constant arch depth because of extraction treatment,and smile esthetics are the same in both groups of patients. Related Articles | Metrics

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TRAb change and prognosis of Graves disease in adolescents before and after 131I therapy 2010, 38 (12):  1099-1100 .  Abstract ( 1652 )   PDF (979KB) ( 2217 )   Purpose Retrospective analysis the serum thyroid stimulating hormone receptor antibody (TRAb) dynamic changes and its relationship with prognosis in adolescent Graves disease(GD) before and after 131I therapy. Methods 140 adolescent GD were treated with 131I. The patients were divided into TRAb-positive group（n=115） and negative group（n=25）before treatment in accordance with levels of TRAb. TRAb levels were measured after treatment 3,6,12,24,36 months in each group. Result Positive group the incidence of hypothyroidism was 10.4% and negative group was 28.0% (P<0.05).The levels of TRAb increased in turn in positive group from hypothyroidism,recurrence,remission,cure before treatment(P<0.01). TRAb of patients with cure reached a peak in 6 months and returned to normal 36 months. Remission reached a peak in 12 months and increased mildly 36 months. Recurrence showed change of M-type characteristics. Hypothyroidism increased markedly after treatment 12 to 24 months and 36 months was still higher than before treatment. The levels of TRAb increased in turn after treatment 36 months from remission, recurrence, hypothyroidism.There were significant differences compared with cure respectively.(P<0.01) Conclusion Dynamic monitoring TRAb levels contribute to clinical outcome and prognosis after 131I therapy adolescent GD .We should be cautious that therapy adolescent GD of TRAb negative with131I. Related Articles | Metrics

临床论丛

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The clinical application of cholecystolithotomy with gallbladder preserved by full laparoscopy combined with choledochoscopy 2010, 38 (12):  1102-1103 .  Abstract ( 1325 )   PDF (985KB) ( 2060 )   Abstract Objective：To explore the effect of cholecystolithotomy with gallbladder preserved by full laparoscopy combined with choledochoscopy for patients with gallstone. Methods：The data of 45 patients receiving cholecystolithotomy with gallbladder preserved by full laparoscopy combined with choledochoscopy from Jun 2008 to May 2009 were retrospectively analyzed. Results：All of these cases were performed successfully by full laparoscopy combined with choledochoscopy . The average operative time was 65±18.5 min.The average postoperative hospital stay was 3.5 days.The complete removal of the stones was proved by choledochoscope and the incision on the gallbladder was closed by sutures by full laparoscopy.No peritoneal drainage was needed. There was no postoperative bile leakage. Conclusion：Compared with other surgical methods with gallbladder preserved, cholecystolithotomy by full laparoscopy combined with choledochoscopy is an effective, safe and more micro-invasive method in treating gallstone while preserving the gallbladder function. Related Articles | Metrics

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Analysis of Pelvic Floor Reconstruction Operation in 28 Cases 2010, 38 (12):  1103-1104 .  Abstract ( 1550 )   PDF (987KB) ( 2023 )   【Abstract】 Objective: To analyze the effect and safety of pelvic floor reconstruction operation with pelvic organ prolapse. Methods: 28 cases with pelvic organ prolapse were operated with pelvic floor reconstruction operation, those who with stress urinary incontinence were operated with tension free vaginal tape. Analyze the effects and complications of the operation．Results: The average operation time was 109min，and the average hemorrhage was 156ml. The average hospitalization was 9.3 days. The average period of follow-up after operation was 1.2 years．The cases with pelvic organ and anterior or posterior vaginal wall prolapse cured basically, the complication were disappeared or improved obviously. After operation anterior vaginal wall I stage prolapse were occurred in one cases. Conclusion: The Pelvic Floor Reconstruction Operation is safe and feasible, which can improve the structures and functions of pelvic floor, and the short-time effect is well, so it is worth promoting in clinic. Related Articles | Metrics

短篇与个案

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The preventive photocoagulation to retinal degeneration before LASIK 2010, 38 (12):  1118-1118 .  Abstract ( 1176 )   PDF (503KB) ( 1979 )   【Abstract】 Objective To investigate methods and the effect of preventive retinal laser photocoagulation on patients with myopia. Methods 2174 eyes with retinal degeneration were found before LASIK and were treated by argon laser photocoagulation. Results Retinal degeneration, hole and retinal detachment were closed well by argon laser photocoagulation after two weeks. They were not developmented after the follow-up lasik. Conclusions Retinal degeneration should be treated by argon laser photocoagulation as soon as possible. Related Articles | Metrics