天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (8): 864-867.doi: 10.11958/j.issn.0253-9896.2015.08.009

• 实验研究 • 上一篇    下一篇

去肾交感神经术对心肌梗死后犬心脏氧化应激和交感神经重构的影响

宋立君，卢成志，李超，张瑾，王丽，马艺杰   

  1. 1天津市第一中心医院消化科（邮编300192），2心内科；3天津医科大学第一中心临床学院
  • 收稿日期:2014-12-17 修回日期:2015-03-11 出版日期:2015-08-15 发布日期:2015-08-15
  • 通讯作者: 卢成志 E-mail:songlijun3337@163.com
  • 作者简介:宋立君(1985)，男，硕士，主要从事高血压病基础和临床研究
  • 基金资助:
    天津市应用基础与前沿技术研究计划（14JCYBJC26100）；天津市卫生局科技基金（2014KY11）

Effect of renal denervation on cardiac oxidative stress and sympathetic nerve remodeling after myocardial infarction in canine

SONG Lijun, LU Chengzhi, LI Chao, ZHANG Jin, WANG Li, MA Yijie   

  1. 1 Department of Gastroenterology of Tianjin First Center Hospital, Tianjin 300192, China; 2 Department of Cardiology of Tianjin First Center Hospital; 3 First Center of Clinical College, Tianjin Medical University
  • Received:2014-12-17 Revised:2015-03-11 Published:2015-08-15 Online:2015-08-15

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摘要： 目的观察去肾交感神经术（RDN）对犬心肌梗死（MI）后心脏氧化应激和交感神经重构的影响。方法将18 只健康犬随机分为3 组：假手术组6 只，只行冠脉造影检查；心肌梗死组（MI 组）6 只，建立MI 模型后1 周行肾动脉造影检查；心肌梗死+RDN 治疗组（MI+RDN 组）6 只，建立MI 模型后1 周行RDN 治疗。明胶海绵栓塞法制作 MI 模型后4 周，通过超声心动图测量各组犬左室舒张末期容积（LVEDV）、左室收缩末期容积（LVESV）、左室射血分数（LVEF）；免疫组化法检测梗死周边区酪氨酸羟化酶（TH）阳性神经纤维分布和密度；检测心肌氧化应激指标：超氧化物歧化酶（SOD）、丙二醛（MDA）含量。结果与假手术组相比，MI 组和MI+RDN 组LVEF 和SOD 降低（P＜ 0.05），LVEDV、LVESV、MDA 和TH 阳性神经纤维相对表达量均增加（P＜0.05）。TH 阳性神经纤维相对表达量与 SOD 呈负相关（rs=-0.818，P＜0.05），与MDA 呈正相关（rs=0.900，P＜0.05）。MI+RDN 组较MI 组LVEF 和SOD 升高（P＜0.05），而LVEDV、LVESV、MDA 和TH 阳性神经纤维相对表达量均降低（P＜0.05）。结论RDN 可以降低心脏氧化应激水平、抑制MI 后心脏交感神经重构、改善心功能。

关键词: 去肾交感神经术, 心肌梗死, 交感神经重构, 氧化应激, 动物, 实验

Abstract: Objective To observe the effect of renal denervation (RDN) on the cardiac oxidative stress and sympathetic nerve remodeling after myocardial infarction (MI) in canine. Methods Canine (n=18) were randomly divided into three groups: Sham operation group (SHAM group, n=6), MI group (n=6), MI+RDN group (n=6). Anterior myocardial infarction was gained by gelatin sponge embolization of the left anterior descending artery. At four weeks post-MI, left ventricular enddiastolic volume (LVEDV), left ventricular end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF) were examined using echocardiography. Immunohistochemical assay was used to analyze the distribution and density of tyrosine hydroxylase (TH) stained nerve fibers at peri-infarct zone. Myocardial superoxide dismutase (SOD) activity and malondialde⁃ hyde (MDA) were also assessed. Results Compared with dogs in SHAM group, LVEF and SOD expression were decreased in MI group and MI+RDN group (P＜0.05), but Left ventricular end diastolic pressure (LVEDP), LVEDV, LVESV, MDA and rate of TH positive staining nerve fibers were increased (P＜0.05). There was a negative correlation between the rate of TH positive staining nerve fibers and SOD level (rs=-0.818，P＜0.05) and a positive correlation between rate of TH positive stain⁃ ing nerve fibers with MDA level (rs=0.900，P＜0.05). By contract, compared with MI group, LVEF and SOD in MI +RDN group were increased (P＜0.05), while LVEDV, LVESV, MDA and rate of TH positive staining nerve fibers were significant⁃ ly lowered (P＜0.05). Conclusion RDN is effective to decrease the level of cardiac oxidative stress and improve cardiac sympathetic nerve remodeling and heart function after myocardial infarction in canine.

Key words: renal denervation, myocardial infarction, sympathetic nerve remodeling, oxidative stress, animals, laboratory