Tianjin Medical Journal

Tianjin Med J ›› 2015, Vol. 43 ›› Issue (5): 484-487.doi: 10.11958/j.issn.0253-9896.2015.05.010

Previous Articles     Next Articles

Apelin-13 protects the cerebral ischemia-reperfusion injury in rats

DONG Xin1, LU Suqing2, LIAO Huiying1, OUYANG Xinping3, LI Guoshu4, ZHOU Jie5△#br# #br#   

  1. 1 Department of Internal Neurology, The First Affiliated Hospital, University of South China, Hunan 421001; 2 Department of Nuclear Medicine, The Affiliated Hospital, Guilin Medical College; 3 Department of Physiology i.e. Institute of Neuroscience, Medical College, University of South China; 4 Department of Emergency, Hengyang Central Hospital; 5 The 169th Hospital of the Chinese People's Liberation Army
  • Received:2014-10-28 Revised:2014-12-02 Published:2015-05-15 Online:2015-05-25
  • Contact: ZHOU Jie E-mail:zhoujie7927@126.com E-mail:dongxin7945@126.com

PDF (PC)

3858

Abstract: Abstract: Objective To observe the protective effect of Apelin- 13 on the cerebral ischemia- reperfusion injury (CIRI), and to explore the possible mechanism in rat model. Methods Fifty male SD rats were randomly divided into five groups: sham group, CIRI model group and Apelin-13 (0.1, 1.0 and 10.0 μg/kg) treatment groups. The model of CIRI was es⁃ tablished by filament. After 2 h ischemia, the focal middle cerebral artery was followed by 72 h reperfusion. Apelin-13 was administrated by intracerebroventricular injection 30 minutes before reperfusion. The score of neural function was estimated in different time points. The 2,3,5- triphenyl tetrazolium chloride (TTC) dye was used to calculate the volume and percent⁃ age of cerebral infarction. The endoplasmic reticulum stress (ERS) protein markers including glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in cerebral cortex were measured by Western blot assay. Results Compared with the sham group, the score of neural function was significantly increased, the infarct rate was reached(47.63 ± 5.81)% and the protein expressions of GRP78 and CHOP were significantly up-regulated in CIRI mod⁃ el group (P<0.05). There were no significant differences in these data between the CIRI model group and 0.1 μg/kg Apelin- 13 treatment group (P>0.05). Compared with the CIRI group, the neural function defect was significantly improved, the mus⁃ cle strength was significantly enhanced and the infarct rate was significantly decreased, and the protein expressions of GRP78 and CHOP were significantly down-regulated in the 1.0 and 10.0 μg/kg Apelin-13 treatment groups (P<0.05). Con⁃ clusion Apelin-13 protects the cerebral ischemia-reperfusion injury in rat model, which may be related with the inhibition of endoplasmic reticulum stress.

Key words: Apelin-13, Cerebral ischemia-reperfusion injury, Nerve function, Cerebral infarction, Endoplasmic reticulum stress, Glucose-regulated protein 78, CCAAT/enhancerbinding protein homologous protein