Abstract: Construction of high level of stably expression of Prestin protein in vitro YU Li Department of Physiology, Fundamental School, Liao Ning Medical University, Jin Zhou Liao Ning 121001 Abstract Objective To construct stable expressions of prestin in CHO cell line in vitro. Methods CHO cells were transfected by prestin plasmids, which were selected by high dose antibiotic. Multi-colony cells which selected by antibiotic were subcultured and measured for expressing of prestin protein. Result It is successful that stably high expression of prestin protein in CHO cell line was constructed. Conclusion Multi-colony highly stably expression of prestin protein in cell line was constructed in which was transfected and selected by a simple, short-term, easy to operate method. The research is also lay a base for next studies on prestin protein.

Key words: prestin, CHO, transfection