Abstract: Abstract Objective :To study the effect of dbcAMP on the Proliferation of SW579 Cell Line.Methods: SW579 cells were treated with dbcAMP (0.5,1.0,2.0 mmol/L) at 24 h. The growth activity of cells was detected by MTT; The activity of MPF through the ELISA; the protein express of the phosphorylation level of cdc2-Tyr15 by Western Blot; the change of cell cycle will be detected by FCM . Results :MTT showed that the growth activity of SW579 cells was reduced ; ELISA showed that dbcAMP could decrease the activity of MPF. The activity of MPF was (115.5±7.53) ng/L in group without dbcAMP ; While the activity of MPF was (22.87±2.57) ng/L in group with dbcAMP . Western blot showed that the content of cdc2-Tyr15 increased. After the treatment with 1.0 mmol/L dbcAMP for 24 h, the percentage of SW579 cell in G0/G1 phase was (62.50±1.22)%, in S phase was (10.15±0.38)%, in G2/M phase was (26.39±0.76)%; otherwhile in the control group, the percentage of SW579 cell in G0/G1 phase was (60.83±1.61)%, in S phase was (29.19±0.97)%, in G2/M phase was (8.75±0.50)%. Conclusion :dbcAMP could significantly inhabit the SW579 cells proliferation and arrest cell cycle in G2/M phase. The mechanism on inhibiting the growth of thyroid cancer maybe related to the decreasing activity of MPF.

Key words: dbcAMP, thyroid squamous cell carcinoma, cell proliferation