Abstract: Abstract Objective: To investigate the levels of estradiol and TNF alpha in the serum of the endometriosis patients with pelvic pain and their correlation. Methods: The levels of estradiol and TNF alpha in the serum of 113 patients with Ems pelvic pain (mild pain group of 37 cases, moderate pain group 41 cases; 35 cases of severe pain group) and 30 women without Ems (control group) were measured with ELISA. Results: No significant difference was found in the levels of estradiol and TNF alpha in the patienrs with mild pain and the normal group(P > 0.05);But in patients with moderate and severe pain,the leves of estradiol and TNF alpha were significantly higher than that of mild pain, and severe pain is higher than that of moderate pain, differences were statistically significant (P < 0.05); In mild pain, estradiol has no correlation with TNF alpha level (P > 0.05), while estradiol in moderate and severe pain is a positive correlation with the expression level of TNF alpha (all P <0.05).Conclusion: The leves of estradiol and TNF alpha in patients with moderate and severe pelvic pain of Ems were significantly higher than normal and mild pain, and the expression was positively correlated, suggests that relatively high concentrations of estradiol can induced macrophage to release TNF alpha , thus play a role in chronic pelvic pain of Ems .

Key words: endometriosis, pelvic pain, estradiol, Tumor necrosis factorα, serum, enzyme linked immunosorbent assay