Abstract:

[Abstract] Objective To investigate expression level of sphingosine kinase1(SPHK1) and nuclear factor-κB (NF- κB) in non-small cell lung cancer (NSCLC) and their relationships with invasion, metastasis and prognosis of NSCLC.Meth? ods Ninety-three NSCLC specimens and paraneoplastic normal lung tissue from conventional surgery were confirmed by histology. Expression of SPHK1and NF-κB were detected by Immunohistochemistry on paraffin sections. Primary antibody were Rabbit Anti-Human SPHK1and Rabbit Anti-Human NF-κB p65, which were incubated 1hour in water bath. The secondary antibody was HRP-Polymer anti Mouse IgG, which was incubated20minutes in water bath.Results SPHK1ex? pression was positive in96.8% (90/93) of NSCLC specimen which is higher than in paraneoplastic normal lung tissue in which the positive rate is18.3% (17/93) ; NF-κB expression was positive in89.2% (83/93) NSCLC which is higher than the in paraneoplastic normal lung tissue in which the positive rate is 12.9% (12/93). The expression of SPHK1and NF-κB in NSCLC was positively correlated (r=0.464, P<0.01). TThe expression levels of SPHK1and NF-κB p65in NSCLC patients with were positively related to TNM staging and lymph node metastasis. SPHK1expression and NF-κB p65expression lev? el were higher in the deads than in survivals. There was no statistical significance in different expression intensity of SPHK1 and NF-κB p65in patients with NSCLC who had differences in gender, age, tumor size, tumor location, histological type. Survival analysis showed that survival time of patients of NSCLC with high expression of SPHK1was shorter than those in the group with low SPHK1expression, and the difference was statistically significant (χ 2 =14.025, P<0.01). Conclusion In the process of NSCLC invasion and metastasis，SPHK1may play an important role through NF-κB, and it can predict prognosis of NSCLC patient. Moreover, it will become a potential target for NSCLC target.

Key words: non-small cell lung cancer , sphingosine kinase 1, nuclear factor κB, prognosis