Study on the effect and mechanism of paclitaxel on pyroptosis in mice with experimental autoimmune encephalomyelitis

doi:10.11958/20221146

Abstract

CLC Number:

R744.51

Figures/Tables 6

References 24

[1]	RODRÍGUEZ MURÚA S, FAREZ M F, QUINTANA F J. The immune response in multiple sclerosis[J]. Annu Rev Pathol, 2022, 17:121-139. doi:10.1146/annurev-pathol-052920-040318.
[2]	GHOLAMZAD M, EBTEKAR M, ARDESTANI M S, et al. A comprehensive review on the treatment approaches of multiple sclerosis:Currently and in the future[J]. Inflamm Res, 2019, 68(1):25-38. doi:10.1007/s00011-018-1185-0.
[3]	AFSHAR B, KHALIFEHZADEH-ESFAHANI Z, SEYFIZADEH N, et al. The role of immune regulatory molecules in multiple sclerosis[J]. J Neuroimmunol, 2019, 337:577061. doi:10.1016/j.jneuroim.2019.577061.
[4]	YAMOUT B I, ALROUGHANI R. Multiple sclerosis[J]. Semin Neurol, 2018, 38(2):212-225. doi:10.1055/s-0038-1649502.
[5]	SMITH P. Animal models of multiple sclerosis[J]. Curr Protoc, 2021, 1(6):e185. doi:10.1002/cpz1.185.
[6]	SHARMA B R, KANNEGANTI T D. NLRP3 inflammasome in cancer and metabolic diseases[J]. Nat Immunol, 2021, 22(5):550-559. doi:10.1038/s41590-021-00886-5.
[7]	WANG L, HAUENSTEIN A V. The NLRP3 inflammasome:Mechanism of action,role in disease and therapies[J]. Mol Aspects Med, 2020, 76:100889. doi:10.1016/j.mam.2020.100889.
[8]	MCKENZIE B A, FERNANDES J P, DOAN M, et al. Activation of the executioner caspases-3 and -7 promotes microglial pyroptosis in models of multiple sclerosis[J]. J Neuroinflammation, 2020, 17(1):253. doi:10.1186/s12974-020-01902-5.
[9]	ALQAHTANI F Y, ALEANIZY F S, EL TAHIR E, et al. Paclitaxel[J]. Profiles Drug Subst Excip Relat Methodol, 2019, 44:205-238. doi:10.1016/bs.podrm.2018.11.001.
[10]	LANGE B M, CONNER C F. Taxanes and taxoids of the genus Taxus-A comprehensive inventory of chemical diversity[J]. Phytochemistry, 2021, 190:112829. doi:10.1016/j.phytochem.2021.112829.
[11]	LI L, JIANG M, QI L, et al. Pyroptosis,a new bridge to tumor immunity[J]. Cancer Sci, 2021, 112(10):3979-3994. doi:10.1111/cas.15059.
[12]	陈庆昌. 多功能纳米复合材料负载MDR-siRNA逆转肿瘤细胞多药耐药性及多金属氧酸盐抑制Aβ聚集的研究[D]. 广州: 暨南大学, 2015.
	CHEN Q C. Reverse multidrug resistance of tumor cells by MDR-siRNA loaded by multifunctional nanocomposite and inhibition of Aβ aggregation by polyoxometalate[D]. Guangzhou: Jinan University, 2015.
[13]	SHENG Z, ZENG J, HUANG W, et al. Comparison of therapeutic efficacy and mechanism of paclitaxel alone or in combination with methotrexate in a collagen-induced arthritis rat model[J]. Z Rheumatol, 2022, 81(2):164-173. doi:10.1007/s00393-020-00940-x.
[14]	WANG X, LIU X, LI Y, et al. Sensitivity to antitubulin chemotherapeutics is potentiated by a photoactivable nanoliposome[J]. Biomaterials, 2017, 141:50-62. doi:10.1016/j.biomaterials.2017.06.034.
[15]	ZENG Q Z, YANG F, LI C G, et al. Paclitaxel enhances the innate immunity by promoting NLRP3 inflammasome activation in macrophages[J]. Front Immunol, 2019, 10:72. doi:10.3389/fimmu.2019.00072.
[16]	GRAF J, ALBRECHT P, GOEBELS N, et al. Ocrelizumab for treatment of multiple sclerosis[J]. Nervenarzt, 2020, 91(8):722-734. doi:10.1007/s00115-020-00937-6.
[17]	VARYTĖ G, ARLAUSKIENė A, RAMAŠAUSKAITĖ D. Pregnancy and multiple sclerosis:An update[J]. Curr Opin Obstet Gynecol, 2021, 33(5):378-383. doi:10.1097/GCO.0000000000000731.
[18]	SYED Y Y. Alemtuzumab:A review in relapsing remitting multiple sclerosis[J]. Drugs, 2021, 81(1):157-168. doi:10.1007/s40265-020-01437-2.
[19]	DISABATO D J, QUAN N, GODBOUT J P. Neuroinflammation:The devil is in the details[J]. J Neurochem, 2016, 139(Suppl 2):136-153. doi:10.1111/jnc.13607.
[20]	ROTHHAMMER V, QUINTANA F J. Control of autoimmune CNS inflammation by astrocytes[J]. Semin Immunopathol, 2015, 37(6):625-638. doi:10.1007/s00281-015-0515-3.
[21]	LIU B, GU Y, PEI S, et al. Interleukin-1 receptor associated kinase(IRAK)-M -mediated type 2 microglia polarization ameliorates the severity of experimental autoimmune encephalomyelitis(EAE)[J]. J Autoimmun, 2019, 102:77-88. doi:10.1016/j.jaut.2019.04.020.
[22]	娄雅琳. Zhx2激活巨噬细胞Nlrp3炎性体诱导焦亡参与EAE的作用及机制研究[D]. 济南: 山东大学, 2021.
	LOU Y L. Study on the role and mechanism of Zhx2-activated macrophage Nlrp3 inflammasome to induce pyroptosis in EAE[D]. Jinan: Shandong University, 2021. doi:10.27272/d.cnki.gshdu.2021.004558.
[23]	ZHEN Y, ZHANG H. NLRP3 Inflammasome and inflammatory bowel disease[J]. Front Immunol, 2019, 10:276. doi:10.3389/fimmu.2019.00276.
[24]	MCKENZIE B A, MAMIK M K, SAITO L B, et al. Caspase-1 inhibition prevents glial inflammasome activation and pyroptosis in models of multiple sclerosis[J]. Proc Natl Acad Sci U S A, 2018, 115(26):E6065-E6074. doi:10.1073/pnas.1722041115.

基因名称	引物序列（5'→3'）	产物大小（bp）
GAPDH	上游：TGAAGGGTGGAGCCAAAAG	227
GAPDH	下游：AGTCTTCTGGGTGGCAGTGAT	227
NLRP3	上游：TCTCAAGTCTAAGCACCAACCG	165
NLRP3	下游：CGAAGCAGCATTGATGGGAC	165
caspase-1	上游：TCAACTCAGTGAGTATAGGGACAAT	292
caspase-1	下游：GATGAAGGATGTCCTCCTTTAGAAT	292

基因名称	引物序列（5'→3'）	产物大小（bp）
GAPDH	上游：TGAAGGGTGGAGCCAAAAG	227
GAPDH	下游：AGTCTTCTGGGTGGCAGTGAT	227
NLRP3	上游：TCTCAAGTCTAAGCACCAACCG	165
NLRP3	下游：CGAAGCAGCATTGATGGGAC	165
caspase-1	上游：TCAACTCAGTGAGTATAGGGACAAT	292
caspase-1	下游：GATGAAGGATGTCCTCCTTTAGAAT	292

组别	潜伏期（d）	高峰期（d）	神经功能障碍评分
EAE模型组	8.00±2.05	14.60±2.07	4.20±0.79
PTX低剂量组	10.80±1.62^a	16.80±1.93^a	2.80±1.14^a
PTX中剂量组	12.90±1.97^ab	19.00±2.62^ab	2.70±1.16^a
PTX高剂量组	14.90±2.42^abc	21.20±2.10^abc	1.90±0.74^a
F	21.020^＊＊	16.728^＊＊	9.614^＊＊

组别	潜伏期（d）	高峰期（d）	神经功能障碍评分
EAE模型组	8.00±2.05	14.60±2.07	4.20±0.79
PTX低剂量组	10.80±1.62^a	16.80±1.93^a	2.80±1.14^a
PTX中剂量组	12.90±1.97^ab	19.00±2.62^ab	2.70±1.16^a
PTX高剂量组	14.90±2.42^abc	21.20±2.10^abc	1.90±0.74^a
F	21.020^＊＊	16.728^＊＊	9.614^＊＊

组别	IL-18	IL-1β
正常对照组	0.28±0.06	17.85±3.47
EAE模型组	0.57±0.09^a	30.00±4.64^a
PTX低剂量组	0.45±0.10^b	24.83±3.71^b
PTX中剂量组	0.36±0.09^bc	20.87±4.02^bc
PTX高剂量组	0.28±0.05^bcd	17.34±2.12^bcd
F	23.214^＊＊	20.551^＊＊

Study on the effect and mechanism of paclitaxel on pyroptosis in mice with experimental autoimmune encephalomyelitis

Cite this article

RichHTML

PDF (PC)

Knowledge

Abstract

share this article

Figures/Tables 6

References 24

Related Articles 15

Recommended Articles

Metrics

Comments

组别	NLRP3	caspase-1
正常对照组	1.00±0.00	1.00±0.00
EAE模型组	3.90±0.59^a	4.57±0.40^a
PTX低剂量组	3.01±0.48^b	3.71±0.43^b
PTX中剂量组	2.53±0.33^b	2.99±0.21^bc
PTX高剂量组	2.09±0.23^bc	2.23±0.59^bcd
F	23.517^＊＊	38.285^＊＊

[1]	LIN Feng, CHEN Lingxiong, LIU Yu, ZHANG Xuming, YIN Zhida, LIN Tanhui, LIU Zunrong. Construction of long term restenosis prediction model for patients with severe subpatellar artery lesions in type 2 diabetes treated with paclitaxel coated balloon [J]. Tianjin Medical Journal, 2024, 52(8): 830-834.
[2]	CHU Jiyan, TIAN Jing, FU Diyu, GUO Lin, SUN Rui, LI Ping. Expression and significance of AIM2 inflammasome in patients with acute gouty arthritis [J]. Tianjin Medical Journal, 2024, 52(5): 518-522.
[3]	WANG Yue, QUAN Xingmiao, WANG Yu, SONG Chunxia, SHAO Yue, XU Liwei. Influence of Yiqi Shengqing recipe on neuron pyroptosis in ischemic stroke rats by regulating HIF-1α/NLRP3 signal pathway [J]. Tianjin Medical Journal, 2024, 52(4): 350-355.
[4]	XIANG Jinjie, LYU Maoxin, WANG Mengyue, ZHANG Kun, LI Hao. Molecular mechanisms of Ca²⁺-induced pyroptosis and adhesion changes of HK-2 cells in the formation of calcium-containing kidney stones [J]. Tianjin Medical Journal, 2024, 52(3): 250-255.
[5]	ZHANG Dingqian, GAO Kefeng, XIONG Luyao, BAI Xue, NIE Dengke. Clinical efficacy of carrellizumab combined with albumin-bound paclitaxel in patients with locally advanced esophageal cancer [J]. Tianjin Medical Journal, 2024, 52(12): 1256-1261.
[6]	OUYANG Jie, ZHAO Haiqian, KONG Yun, NIU Qin, CHEN Ying, SI Yongyu. The effect of electroacupuncture on paclitaxel-induced neuropathic pain in rats [J]. Tianjin Medical Journal, 2024, 52(11): 1141-1145.
[7]	LIU Guoqi, LI Chengcheng, LIU Shengju, ZHU Liying. Emodin regulates histone acetylation to promote pyroptosis and apoptosis of HpG2 hepatoma cells [J]. Tianjin Medical Journal, 2024, 52(1): 56-60.
[8]	WANG Xiaohong, QIAN Jing, WANG Jiawei, QI Xiaoming, MENG Yun, WANG Ping, CHENG Ruizhi, ZHOU Guoxiong. The study on the mechanism of salidroside in the adjuvant treatment of patients with moderately severe acute pancreatitis [J]. Tianjin Medical Journal, 2023, 51(7): 762-765.
[9]	SUN Liyan, LIU Zeru, SU Yongsheng, AI Hongliang. Effect of asperuloside on pyroptosis of lung tissue in septic rats by regulating NLRP3/Caspase-1/GSDMD signaling pathway [J]. Tianjin Medical Journal, 2023, 51(6): 607-612.
[10]	HU Yingshan, WANG Jingjing, GAO Hongmei. Research progress in the pathogenesis of sepsis-related intestinal dysfunction [J]. Tianjin Medical Journal, 2023, 51(3): 333-336.
[11]	LIU Shujuan, LIU Mengying, SU Wuyun, DOU Jia, WANG Wei. Investigation on the determination of tumor-associated macrophages inducing the drug resistance of albumin-bound paclitaxel in triple-negative breast cancer cells by activating IGF-1R signaling pathway [J]. Tianjin Medical Journal, 2023, 51(11): 1158-1163.
[12]	LIU Chang, FAN Caihong, LIU Jia, LIU Yashan, ZHANG Shiqi, LIU Shiming, SHEN Yanna. Naa20 alleviated myocardial injury in sepsis by inhibiting AIM2 expression [J]. Tianjin Medical Journal, 2023, 51(10): 1065-1070.
[13]	ZHAO Zilong, ZHANG Meiyun, GAO Hui, DU Wei, BA Yaer, LYU Yihua△. The clinical efficacy and safety of anlotinib and albumin-bound paclitaxel as the third-line therapy in patients with non-small cell lung cancer cancer [J]. Tianjin Medical Journal, 2022, 50(5): 539-543.
[14]	LI Juan, YUAN Xiang-fei, ZHAO Yu. Relationship between NLRP3 and inflammatory molecules expression in intestinal mucosa cells of mice with FPIES [J]. Tianjin Medical Journal, 2021, 49(8): 829-832.
[15]	LIU Yu, , WANG Zhao-yang, LI Shi-peng, HU Sha-sha, , YANG Shuang, CAI Jin-zhen, ZHANG Guo-liang△ . Interferon regulatory factor-1 participates in hepatic ischemia reperfusion injury in mice by regulating pyroptosis [J]. Tianjin Medical Journal, 2021, 49(5): 465-471.