A study of information transmission in HPC-PFC network during working memory encoding induced by propofol anesthesia

doi:10.11958/20230307

Abstract

Abstract:

Objective To figure out whether propofol anesthesia impairs information transmission from hippocampal to prefrontal cortex (HPC-PFC) neural pathways during the coding phase of working memory in rats. Methods Among 12 rats, 6 SD rats (3 months old) were selected for research analysis, and 16-channel microelectrode arrays were implanted into the medial prefrontal cortex (mPFC) and the ventral hippocampus (vHPC) of rats, respectively. The Cerebus information acquisition system was used to record the working memory coding stage tasks of each rat before and 12 and 24 hours after anesthesia with 150 mg/kg propofol. The multichannel local field potential (LFPs) signals of mPFC and vHPC, two responsible brain regions, were used to establish the vHPC-mFPC network. The directional transfer function (DTF) and the information flow of vHPC-mPFC neural pathway in vHPC and mPFC networks were calculated respectively, and the information transmission of vHPC-mPFC neural pathway before and after anesthesia was quantitatively characterized. Results During working memory encoding, information flow from vHPC to mPFC was significantly reduced 12 h after propofol anesthesia comparison with before propofol anesthesia, but it recovered at 24 h after propofol injection. There were no significant differences in the average vHPC and mPFC network connection strength and vHPC-mPFC information flow of rats between before anesthesia and 24 h after anesthesia. Conclusion These results indicated that working memory impairment induced by propofol may result from disrupting information transmission in vHPC-mPFC network during working memory encoding.

Key words: propofol, hippocampus, cerebral cortex, frontal lobe, electroencephalography, working memory encoding

CLC Number:

R318

Figures/Tables 6

Fig.1 Y maze for rat behavioral experiment

Fig.2 Schematic diagram of multielectrode arrays implanted in mPFC and vHPC

Fig.3 Histological result of rat brain

Fig.4 Average network connection strength of vHPC and mPFC

Fig.5 Comparison of DTF before anesthesia and 12 h, 24 h after anesthesia. ＊＊P<0.01。

Fig.6 Comparison of information flow before anesthesia and at 12 h, 24 h after anesthesia. ＊＊P<0.01。

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