Tianjin Medical Journal ›› 2023, Vol. 51 ›› Issue (9): 968-971.doi: 10.11958/20230001

• Experimental Research • Previous Articles     Next Articles

Effects of mild hypothermia treatment on coagulation function and inflammatory factors in a swine model after cardiac arrest and extracorporeal cardiopulmonary resuscitation

WANG Jinxiang1(), XU Guowu1, JIN Heng1, HE Bin2, CHAI Yanfen1,()   

  1. 1. Department of Emergency Medicine, Tianjin Medical University General Hospital,Tianjin 300052, China
    2. Department of Oncology, the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine
  • Received:2023-01-05 Revised:2023-04-18 Published:2023-09-15 Online:2023-09-13
  • Contact: △E-mail:chaiyanfen2012@126.com

Abstract:

Objective To investigate effects of mild hypothermia treatment on coagulation function and inflammatory factors in a swine model of extracorporeal cardiopulmonary resuscitation (ECPR) after cardiac arrest (CA). Methods Using random number table method, twelve Bama pigs were divided into the control group, the normal temperature group and the mild hypothermia group with four pigs in each group. CA model was established by right ventricular fibrillation in the normal temperature group and the mild hypothermia group, and then ECPR was performed by extracorporeal membrane oxygenation (ECMO). The target temperature of the normal temperature group was 37 ℃, and the mild hypothermia group was 34 ℃. Control group was only given conventional tube placement. Pigs were killed 24 hours later, and venous blood was taken to detect levels of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB) and D-dimer antigen (D-D). Expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme linked immunosorbent assay (ELISA). Results Compared with the control group, PT, APTT, TT, FIB, D-D and expression levels of TNF-α and IL-6 were significantly increased in the normal temperature group and the mild hypothermia group. Compared with the normal temperature group, the above indicators were decreased significantly in the mild hypothermia group (P<0.05). Conclusion Mild hypothermia treatment can significantly improve coagulation function and reduce the release of inflammatory factors in a swine model of ECPR after CA, so as to alleviate the occurrence of ECPR-related complications and inflammatory reactions.

Key words: mild hypothermia, cardiac arrest, extracorporeal cardiopulmonary resuscitation, coagulation function, inflammatory factor

CLC Number: