Abstract: Objective To study the therapeutic effect of Liangxue Xiaoyin granule on psoriasis transgenic mice (K14-VEGF) and its effect on the miR-155/cytokine signal transduction inhibitor 1 (SOCS1) axis. Methods　Eight untreated Swiss outbred line (FVB/NJ) mice were randomly selected as the control group. In addition, 24 SPF K14-VEGF transgenic mice were according to random number table method divided into model group, Liangxue Xiaoyin granule group and methotrexate group, 8 in each group. The control group and model group were intragastrically administered normal saline, Liangxue Xiaoyin granule group was intragastrically administered at a dose of 60 g/kg, methotrexate group was intragastrically administered at a dose of 2 mg/kg, once a day for 10 days. The severity index (PASI) score of psoriasis was observed, and the morphological changes of cells were observed by HE staining. The expression levels of serum TNF-α, IL-23, IL-6 and IL-1 were detected by enzyme-linked immunosorbent assay (ELISA). The real-time fluorescence quantitative analysis (qPCR) was used to detect the expression of miR-155, and the expression of SOCS1 protein was determined by Western blot (WB) assay. Results　Compared with the control group, the model group showed spinous layer thickening and superficial dermal lymphocyte infiltration. Compared with the model group, the pathological severity of mice decreased significantly, and the cell morphology recovered in the Liangxue Xiaoyin granule group and the methotrexate group. Compared with the control group, the PASI score, the expression levels of TNF-α, IL-23, IL-6, IL-1 and miR-155 were significantly higher, the expression level of SOCS1 was decreased significantly in the model group (P < 0.05). Compared with model group, the PASI score, the expression levels of TNF-α, IL-23, IL-6, IL-1 and miR-155 were decreased significantly in Liangxue Xiaoyin granule group and methotrexate group (P < 0.05), and the expression level of SOCS1 was significantly higher (P < 0.05). There was no significant difference between Liangxue Xiaoyin granule group and methotrexate group (P＞0.05). Conclusion　Liangxue Xiaoyin granule may alleviate the inflammatory reaction and alleviate the symptoms of psoriasis transgenic mice by inhibiting the activation of the miR-155/SOCS1 axis.

Key words: psoriasis, microRNAs, suppressor of cytokine signaling 1 protein, tumor necrosis factor-alpha, interleukins, Liangxue Xiaoyin granule, miR-155