Abstract: Abstract Objective:To establish clinically relevant individuation nude mouse models of human non-small cell lung cancer,which can be stably passaged,so as to lay a foundation for the future individualized therapy study. Methods: Tumor implanting group: BALB/C nude mouse subcutaneously injected in the back with diameter 2mm tumor derived from human operative specimens were killed when the tumor mass grown to 800mm3.This is the first generation.Then,to establish the second generation, BALB/C nude mouse were injected in the back with diameter 2mm tumor derived from first generation tumor mass.the same method redone to gain the third generation tumor. Cell lines implanting group: BALB/C nude mouse subcutaneously injected in the back with 1×106 human lung cancer cell lines A549 or H1795 were killed when the tumor mass grown to 500mm3.The tissue of primary human lung cancer 、first 、second 、third generation implanting tumor and the cell lines implanting group tumor were formalin-fixed、cut sections、HE stained,and then compared with the morphology by light microscope.Results: The tumor cells of the tumor implanting group not only retains the atypia of cancer cells, but also keep original arrangement structure; The cell lines implanting group tumor cells lose their arrangement structure, keeping only the atypia of the cancer cells. Conclusion: Using different surgical excision of lung cancer established human lung cancer tumor nude mice-model which have individual characteristics,this model can be useful for the future individualized therapy study in vivo.

Key words: Models, Animal, Xenograft Model, Carcinoma, Non-Small-Cell Lung