Abstract:

[Abstract]   Objective  To study sperm mRNA transcript expression of CRISP2gene in the semen of patients with
different types of spermatogenetic failure. Methods  A total of150male infertility patients were divided into normal group,asthenozoospermia group and oligozoospermia group（n=50for each group）. Total RNA was extracted from sperm cells. Re-al-time quantitative reverse transcription polymerase chain reaction (qRT- PCR) method was used to detect CRISP2gene mRNA levels in three groups.   Results  There was a significantly higher expression of CRISP2in normal group (10.281±2.173) than that of asthenozoospermia group (2.092±0.969, P<0.05). There was no significant difference in the expression of CRISP2between normal group and oligozoospermia group (9.420±2.794, P>0.05). The relative expression of CRISP2was significantly lower in asthenozoospermia group than that of oligozoospermia group (P<0.05).   Conclusion  The CRISP2 expression was significantly different in patients with different types of spermatogenic failure. The reduced expression of CRISP2may lead to the decreased sperm motility.

Key words: Spermatogenesis, RNA, RNA, messenger, gene expression, cysteine-rich secretory protein2