Abstract:

[Abstract]   Objective   To observe selective cyclooxygenase-2(COX-2) inhibitor celecoxib on the cell migration,
MMP-9and VEGF expression of human gastric cancer SGC-7901.   Methods   In vitro culture of SGC-7901cells, the cells were divided into blank control group and celecoxib treatment group (different concentrations of celecoxib25, 50and 100μmol/L). SGC-7901cells were treated with celecoxib for24h. The effect of celecoxib on cell migration of SGC-7901 was detected by the damage repair experiment. RT-PCR assay was used to detect the effect of celecoxib on MMP-9mRNA and VEGF mRNA expressions in SGC-7901cells.  Results   The migration distance of SGC-7901cells was decreased with the increased concentration of celecoxib. There were significant differences between medium-dose and high-dose celecoxib groups and the control group. RT-PCR assay showed that MMP-9mRNA and VEGF mRNA expressions in gastric cancer SGC-7901were significantly decreased with the increased concentration of celecoxib, and there was a significant difference compared with those of control group  (P<0.05).  Conclusion   Celecoxib can inhibit migration of human gastric cancer SGC-7901cells, which may be related to the inhibition of COX-2downstream of MMP-9and VEGF expressions.

Key words: stomach neoplasms, prostaglandin-endoperoxide synthases, matrix metalloproteinase9, vascular endothelial growth factors, celecoxib