Abstract:

[Abstract]   Objective   To investigate the protective effects and mechanisms of rosiglitazone on lung injury in severe
acute pancreatitis (SAP).  Methods   Seventy- two SD rats were randomized into three groups: sham operation (SO) group,SAP group and rosiglitazone-pretreated group. The model of SAP was induced by retrograde injection of5% sodium taurocholate into the bili-pancreatic duct in SD rats. Rats of rosiglitazone-pretreated group were given10mg/kg rosiglitazone in?traperitoneally 30min before inducing SAP. The amylase plasma levels, levels of TNF-αandp（O2）, the myeloperoxidase (MPO) and the wet/dry ratio of lung tissue were measured. The expression of NF-κB in pulmonary tissues was assayed by immunohistochemistry. The expressions of TNF-αmRNA and intercellular adhesion molecule1(ICAM-1) mRNA in pulmonary tissues were detected by reverse transcript PCR (RT-PCR). The histopathological changes of pulmonary tissues were evaluated.  Results   Compared with SO group, the plasma levels of amylase and TNF-α, the intrapulmonary MPO were significantly increased in SAP group (P＜0.05). The expression levels of NF-κB, TNF-αmRNA and ICAM-1mRNA in lung tissue were also significantly increased in SAP group (P＜0.01). There were significantly lower levels of the above indicator in rosiglitazone-pretreated group than those of SAP group (P＜0.05). Compared with SO group, the lung wet/dryratio was significantly higher at6h and12h in SAP group (P＜0.05). The values of lung wet/dry ratio were significantly lower at 6h and 12h in rosiglitazone-pretreated group than those of SAP group (P＜0.05). The lung tissue damage was significantly reduced. The level ofp（O2）was significantly decreased in SAP group than that of SO group (P＜0.05). The level of p（O2）was significantly increased at6h and12h in rosiglitazone pretreated group than that of SAP group (P＜0.05).   Conclusion   Rosigli?tazone might have protective effects on SAP-related lung injury by inhibiting NF-κB and decreasing the production of TNF-αand ICAM-1in the lung tissues

Key words: pancreatitis, acute necrotizing, respiratory distress syndrome, adult, NF-kB, disease models, animal, 大鼠