Abstract: Abstract   Objective   Exploring biological effects of stathmin silencing on nasopharyngeal carcinoma 5-8F cell line. Methods    Cell proliferation and cell cycle were analyzed by MTT experiment and Flow Cytometry. Cell tumorigenicity was analyzed by nude mouse xenograft experiment. Results    The cell proliferation inhibition rate of stathmin-silenced 5-8F cells was significantly higher than that of negative and blank control group cells (p<0.01). The cell cycle of stathmin-silenced 5-8F cells was significantly arrested in phase G2 compared with that of negative and blank control group(P<0.05). The transplanted tumor weight of stathmin-silenced 5-8F cells in nude mice was (0.32±0.21)g，significantly lower than that of negative control group (1.10±0.40)g and blank control group(1.52±0.30)g(P<0.05). Conclusion   Stathmin-silencing inhibits malignant phenotype of nasopharyngeal carcinoma 5-8F cell line.

Key words: nasopharyngeal neoplasms, Cell line, Tumor, gene silencing, flow cytometry, cell proliferation, neoplasm transplantation, stathmin