Abstract: [Abstract] Objective To investigate Pulsatilla saponin D joint sorafenib on human hepatoma cell line invasion and metastasis of influence. Methods Human hepatocellular carcinoma cell BEL-7402 into Pulsatilla saponin D single-drug treatment group to compare three treatments sorafenib monotherapy treatment group and the two-drug combination treatment group, observation methods on liver cancer cell invasion and metastasis.Results detect the adhesion inhibition rate in each group, the role of 3h, sorafenib group was significantly higher than Pulsatilla saponin D group (P <0.05 or <0.01), the combined treatment group was significantly higher than the sorafenib group and Pulsatilla saponin D group ( P <0.01); the role of 5h, Pulsatilla saponin D group was significantly higher than the adhesion inhibition sorafenib group (P <0.01), and higher than Pulsatilla saponin D group action 3h (P <0.01), described the two drugs on liver cancer cell adhesion synergistic inhibition rate (Q = 2.33> 1.15); detecting migration inhibitory rate in each group, sorafenib monotherapy group and the two-drug combination group of hepatoma cell migration inhibition were significantly higher than Pulsatilla saponin D monotherapy group (P <0.01), and the inhibition rate of two-drug combination group was significantly higher than that of sorafenib monotherapy group (P <0.01), described the two-drug combination migration of hepatoma cell line has a synergistic effect (Q = 1.39> 1.15); Detection of invasion inhibition rate, sorafenib monotherapy group and the two-drug combination group were significantly higher than Pulsatilla saponin D group (P <0.01), described the two drugs on liver cancer cell invasion inhibition of antagonistic effect (Q = 0.68 <0.85). Each group of VEGF-C, MMP-9 expression: MMP-9 expression levels Pulsatilla saponin D monotherapy significantly decreased sorafenib group and two-drug combination group VEGF-C, MMP-9 expression was significantly decreased (P <0.05); compared with Pulsatilla saponin D monotherapy group, VEGF-C expression levels of the two-drug combination group was significantly decreased; two-drug combination group Baitouweng saponin D monotherapy VEGF-C level decreased significantly (P <0.05). Conclusion Pulsatilla saponin D, sorafenib monotherapy as well as combination of two drugs on liver cancer cells BEL-7402 cell line adhesion, migration, invasion has a certain extent, and both hepatoma cell adhesion, migration inhibitory synergistic .

Key words: Pulsatilla saponin D, sorafenib, hepatoma cells, Invasion, Metastasis