Abstract: Abstract: Objective To preliminarily study the anti-colon mechanism of the new compound OSU-03012 at cellular level. Methods The human colon cancer cell line HCT-116, which was intervened with 1 μmol /L, 3 μmol /L, 5 μmol /L and 10 μmol /L of OSU-03012, was used as subjects. HCT-116 cells treated with the same volume of dimethyl sulfoxide (DMSO) were taken as the control group. The inhibition rates of cells in each group after 24, 48 and 72 hours of intervention were detected by CCK-8 method, and the migration ability of cells was detected by scratching test and Transwell assay. The apoptosis of cells was detected by flow cytometry assay, and the expressions of Bcl-2 and Caspase-3 proteins were detected by immunofluorescence assay and Western blot assay. The expressions of Bcl-2 and Caspase-3 mRNA were detected by Real-time PCR. Results The cell inhibition rate and apoptosis rate gradually increased while the cell migration distance and cell migration number gradually decreased in the control group, 1 μmol/L group, 3 μmol/L group, 5 μmol/L group and 10 μmol / L group. There were statistical significance in the multiple comparison between the groups (all P＜0.05). The mRNA and protein expression of Bcl-2 were lower in 3 μmol/L group, 5 μmol/L group and 10 μmol/L group than those in the control group, while mRNA and protein expression of Caspase-3 were higher than those in the control group (both P＜ 0.05). Conclusion The new compound OSU-03012 can inhibit the proliferation and migration of colon cancer cells and promote the apoptosis of colon cancer cells in a dose-dependent manner.

Key words: colonic neoplasms, cell proliferation, cell movement, apoptosis, OSU-03012, cyclooxygenase-22