Abstract: Abstract Objective: To explore the effects of shenmai injection(SM) on lipid peroxidiation in the lung induced by intestinal ischemia/reperfusion(I/R). Methods: 24 SD rats were randomly divided into three groups:sham group,I/R group,treatment group. In sham group ,superior mesenteric artery was separated only;Models of intestinal I/R injury in I/R group and treatment group were estabished. In treatment group, SM(0.9mL/100g) was injected through tail vein before ischemia, before reperfusion and after reperfusion 30min respectively. The state of leukocyte adhesiveness/aggregation(LAA) in peripheral blood, lung wet/dry weight ratio,lung coefficient, Superoxide dismutase(SOD), malondialdehyde(MDA) , myeloperoxidase (MPO) in serum and lung tissue were measured, as well as the effects of SM were observed. The changes of lung histology were examined by light microscope. Results: (1)In I/R group ,LAA in peripheral blood, lung wet/dry weight ratio,lung coefficient were significantly increased than that in sham group(P<0.01); the damage to lung tissue was increased in severity with light microscope. The above-mentioned indexes of treatment group were obviously lower in comparison with I/R group(P<0.01).(2)The activity of SOD in serum and lung tissue were significantly lower in I/R group than that in sham group (P<0.01); The content of MDA , the activity of MPO in serum and lung tissue were significantly increased in I/R group than that in sham group (P<0.01).The above-mentioned indexes of treatment group were obviously ameliorated in comparison with I/R group(P<0.01). Conclusion: Shenmai injection can protect the lung injury induced by intestinal ischemia/reperfusion(I/R) ,which may be mediated by inhibiting lipid peroxidation and aggregation of neutrophils.

Key words: reperfusion injury, acute lung injury, lipid peroxidation, neutrophil, shenmai injection