Abstract: Objective   To investigate the effects of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on the follicular survival and angiogenesis after ovarian tissue heterotopic transplantation by mouse model. Methods   Ovarian tissues isolated from 18-day-old ICR outbred mice were transplanted into subcutaneous part of nuchal region of adult female mice. They were divided into four groups: A) injected with 10μl DPBS as control; B) injected with 10μl of bFGF (50μg/ml); C) injected with 10μl of VEGF (25μg/ml); D) injected with 10μl of bFGF (50μg/ml) + VEGF (25μg/ml). We injected subcutaneously around the grafted ovarian tissue once a day for four days. The grafts were retrieved 7 days after transplantation. The number and morphology of follicles were observed by H.E. staining and vascular density by IHC staining of anti-CD31 antibody. Results   The number of primordial follicles in group B and group C (17.25 ± 2.55; 18.88 ± 2.42 respectively) was significantly higher than that in group A (13.25 ± 2.49) (P < 0.01), while there was no statistical difference between group B and group C; the number of primordial follicles in group D (22.50 ± 3.42) was higher than that in group B and group C (P < 0.05). Compared with the control group, the vascular density in group B and group C increased, significantly higher than group A (P <0.05); but there was no statistical difference between group B and group C; vascular density in group D were statistically higher than that in group B and group C respectively (P < 0.05). Conclusion   Intervention with VEGF and / or bFGF promoted the angiogenesis after ovary tissue transplantation, while reducing the loss of follicles. That benefits improving the quality of ovarian tissue survival after transplantation.

Key words: Ovary, transplantation, homologous, Vascular endothelial growth factors, Fibroblast growth factor-2, ovarian follicle, neovascularization, 病理性