Effect of intra-striatal injection of α-Synuclein PFF on pathological changes of olfactory bulb in macaca fascicularis

doi:10.11958/20221892

Abstract

Abstract:

Objective To investigate whether pathological α-Synuclein (α-Syn) can be generated after striatal injection of α-Syn preformed fibrils (PFF), and then spread from striatum to olfactory bulb, thereby causing neuronal damage in olfactory bulb. Methods Three healthy female macaca fascicularis injected with α-Syn PFF into the bilateral striatum were used as the experimental group. 300 μg α-Syn PFF (7 g/L) was injected into each side of the striatal putaminal nucleus with 60 μg in head, 60 μg in body and 30 μg in tail. The other two healthy female macaca fascicularis were injected with PBS as the control group. Two years after the injection, macaca fascicularis were killed. Pathological changes were detected by Nissl staining, and immunohistochemistry was performed to examine the expression levels of phosphorylated α-Synuclein (pS129), tyrosine hydroxylase (TH) and doublecortin (DCX) in olfactory bulb. Results Compared to the control group, pS129 positive aggregates were found in olfactory bulb of the experimental group. Besides, the number of TH-positive and DCX-positive neurons were significantly decreased in the experimental group [TH:(27.00±11.22) vs. (65.80±36.54); DCX: (67.60±17.23) vs. (88.30±19.89)]. Conclusion Pathological α-Syn can spread from striatum to olfactory bulb and induce the loss of dopaminergic neurons, inhibiting the generation of newborn neurons in olfactory bulb of macaca fascicularis.

Key words: Parkinson disease, alpha-synuclein, olfactory bulb, macaca fascicularis, immunohistochemistry

CLC Number:

R742.5

Figures/Tables 4

References 18

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