Effect and mechanism of early exercise intervention on cerebral nerve myelin in rats with cerebral ischemia

doi:10.11958/20231795

Abstract

Abstract:

Objective To investigate the effect and potential mechanism of early exercise intervention on cerebral myelin in cerebral ischemia rats. Methods A total of 18 SD rats were randomly divided into the sham group, the middle cerebral artery occlusion resting group (MCAO-SED) and the middle cerebral artery occlusion exercise group (MCAO-EX), with 6 rats in each group. Except the sham group, the middle cerebral artery occlusion model was prepared by modified Longa line embolization method in other groups. After modeling, rats in the MCAO-EX group were placed on a treadmill for exercise intervention for 28 days. Neurological function was assessed by modified neural function deficit score (mNSS). Infarct volume was detected by MRI scanning (T2), myelin basic protein (MBP) expression was detected by immunofluorescence. Myelin sheath structure was detected by transmission electron microscopy. Western blot assay was used to detect endoplasmic reticulum stress-related protein expression. Apoptosis was detected by TUNEL staining. Results After 28 days of intervention, compared with the MCAO-SED group, the nerve function recovered well in the MCAO-EX group, infarct volume decreased, myelin integrity increased, MBP fluorescence intensity expression increased and MBP expression level increased. The expression levels of ATF6, p-IRE1, p-PERK and cleaved caspase 3 were significantly decreased, and apoptosis was reduced. Conclusion Early exercise can inhibit endoplasmic reticulum stress-induced apoptosis, promote cerebral myelin repair, reduce infarct size and improve nerve function.

Key words: brain ischemia, myelin sheath, endoplasmic reticulum stress, apoptosis, early exercise intervention

CLC Number:

R743.33

Figures/Tables 9

References 21

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组别	第1天			第3天		第7天
SHAM组	1.66±0.81			0.17±0.41		0.00±0.00
MCAO-SED组	12.33±1.86^a			9.50±2.26^a		8.33±1.21^a
MCAO-EX组	12.17±1.60			8.50±1.38		6.33±1.03^b
组别		第14天	第21天		第28天
SHAM组		0.00±0.00	0.00±0.00		0.00±0.00
MCAO-SED组		6.17±1.33^a	5.17±0.98^a		4.67±0.82^a
MCAO-EX组		4.33±1.03^b	3.67±0.82^b		3.33±0.52^b

组别	第1天			第3天		第7天
SHAM组	1.66±0.81			0.17±0.41		0.00±0.00
MCAO-SED组	12.33±1.86^a			9.50±2.26^a		8.33±1.21^a
MCAO-EX组	12.17±1.60			8.50±1.38		6.33±1.03^b
组别		第14天	第21天		第28天
SHAM组		0.00±0.00	0.00±0.00		0.00±0.00
MCAO-SED组		6.17±1.33^a	5.17±0.98^a		4.67±0.82^a
MCAO-EX组		4.33±1.03^b	3.67±0.82^b		3.33±0.52^b

组别	n	干预前	干预第28天
SHAM组	3	0.00±0.00	0.00±0.00
MCAO-SED组	3	41.23±0.87^a	30.36±1.40^a
MCAO-EX组	3	41.42±1.13	22.04±0.21^b
F		0.053	102.521^＊＊

组别	n	干预前	干预第28天
SHAM组	3	0.00±0.00	0.00±0.00
MCAO-SED组	3	41.23±0.87^a	30.36±1.40^a
MCAO-EX组	3	41.42±1.13	22.04±0.21^b
F		0.053	102.521^＊＊

组别	ATF6	IRE1	p-IRE1	PERK	p-PERK	cleaved caspase 3	MBP
SHAM组	1.00±0.25	1.00±0.23	1.00±0.33	1.00±0.17	1.00±0.11	1.00±0.09	1.00±0.09
MCAO-SED组	2.16±0.33^a	1.05±0.13	1.96±0.20^a	0.95±0.19	1.92±0.27^a	2.86±0.88^a	0.50±0.08^a
MCAO-EX组	1.45±0.02^b	1.05±0.17	1.45±0.27^b	1.02±0.13	1.15±0.28^b	1.60±0.45^b	0.75±0.19^b
F	24.841^＊＊	0.091	12.710^＊＊	0.163	17.6743^＊＊	50.112^＊＊	13.867^＊＊